Statement of Work.docx
DOCX document 32 KB Posted
- Attached to
- Gene Knockout Federal contract opportunity
- Solicitation number
- 12305B23Q0198
About this file
This statement of work outlines gene knockout services required by the US Department of Agriculture Agricultural Research Service. The contractor will be expected to knockout two soybean genes identified by the government as controlling rhizobium nodulation, verify the knockout, and provide T1 seeds from two soybean mutant lines. The period of performance is 11 months from receipt of gene information and funding. Deliverables will include plasmids, T1 seeds, glycerol stocks, and a project report. The goal is to validate genes that reduce the need for nitrogen fertilizer and improve soybean nodulation and nitrogen fixation efficiency.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Contract Terms and Conditions.docx | DOCX document | |
| Past Performance.docx | DOCX document | |
| Wage Determination.pdf |
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Text version
STATEMENT OF WORK (SERVICES) (SOW)
GENERAL INFORMATIONTitle of Project:
Characterization and Utilization of Genetic Diversity in Soybean and Common Bean and Management and Utilization of the National Rhizobium Genetic Resource Collection Statement of Need and Purpose:
The purpose of this request is to verify that the genes we identified are those that control the rhizobium nodulation on soybean roots.
Background Information and Objective:
Reducing the need for nitrogen fertilizer is essential for low-input sustainable agriculture and protecting waterways. Our lab will identify novel genes controlling rhizobium nodulation and determine the underlying mechanism controlling the trait. The new knowledge of rhizobium and host plant interactions will result in the improvement of soybean nodulation and enhanced nitrogen-fixation efficiency.
Through field testing, genetic mapping and sequence analyses, we identified candidate genes that control rhizobium nodulation on soybean, thus we need to knockout these genes in order to validate that these genes are responsible for the nodulation. This proposed work is relevant to the NP301 Action Plan, Component 1. Crop Genetic Improvement; Problem Statement 1A: Trait discovery, analysis, and superior breeding methods. they are also relevant to the NP301 Action Plan Component 2, Plant and microbial genetic resource and information management; Problem Statement 2B: Microbial Genetic Resource and Information Management.
Period of Performance:
The period of performance for this contract will be within 11 months after the gene information and the fund are transferred to the contractor.
SCOPE OF WORKGeneral Requirements:
Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work below:
Specific Requirements:
The contractor shall knockout two genes, verify gene’s knockout, and provide T1 seeds from two soybean mutant lines.
LEVEL OF EFFORT:
Senior researcher or technical researcher.
GOVERNMENT RESPONSIBILITIES
The government shall provide the gene sequences and variants for the two soybean genes to the contractor to enable the performance of the work detailed above.
DELIVERY OR DELIVERABLES
The contractor shall provide plasmids, T1 seeds, glycerol stocks and project report.
REPORTING REQUIREMENTS
The contractor shall submit via e-mail as PDF or a MS Word document (enter what is applicable) a progress report during the contract period.
OTHER CONSIDERATIONS
Data Rights:
If applies ADD
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