Statement of Work.docx

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DNA Sequencing for 30 C Mucosospermus Federal contract opportunity
Solicitation number
12405B21-1035632
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery

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Statement of work

We are looking to sequence 30 watermelon samples using Next Generation Sequencing (NGS) technology with Novaseq platforms (eg. Novaseq 6000). We will need at least 30X coverage of the genome (425 Mb) of sample which is 13 G of raw sequence data for each of the 30 watermelon samples from paired end reads (PE) of PE 150 or higher. This will include whole genome library preparation, short insert DNA (<500bp). Basic data quality control will also be needed, including filtering reads containing adapter or with low quality. We will provide high quality of the 30 watermelon DNA samples for this process. The watermelon genome is about 425 Mb and reference sequences are available. The DNA samples do not contain any known infectious agents or viruses.

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