Statement of Work Channel Catfish Single Cell RNA Sequencing.docx

Statement of work – single cell RNA sequencing of six channel catfish spleen samples

We have 3 whole spleen channel catfish tissues and 3 B-cell sorted spleen channel catfish samples. For each sample, sample processing, high-thruput sequencing, and bioinformatics service to be performed equivalent or near equivalent to Azenta Life Sciences custom 10X Genomics service as follows:

1. Nuclei isolation

2. Sample quality control measures

3. 10X Genomics Chromium 3’ gene expression RNA sequencing library preparation and 10X Genomics library sequencing using Illumina sequencers

a. For the 3 whole spleen samples, a minimum analysis of 6000 cells at 25,000 reads per cell is required

b. For the 3 B-cell sorted spleen samples, a minimum analysis of 3000 cells at 50,000 reads per cell is required

4. Bioinformatics service to include at minimum: interactive differential gene expression analysis through the 10X Genomics Cell Ranger and Loupe Cell Browser using the output data with the channel catfish genome sequence (IpCoco 2.1 or 2.0 version)

5. Deliverables will include a quality report, raw files in FASTQ format, and associated bioinformatics knowhow