Statement of Work 1011819.pdf
PDF 288 KB Posted
- Attached to
- Single Cell RNA Sequencing Service Federal contract opportunity
- Solicitation number
- 12305B20Q1011819
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Service Provisions FAC 2020 08 August 13 2020.pdf | ||
| 52.204-24 Representation.pdf |
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Text version
Minimum requirements for single cell RNAseq service on 4 cattle samples:
1. Frozen cattle cells (4 samples) will be delivered on dry ice for analysis
2. Cell preparation (cattle): thawing, washing and counting
3. Dead cell removal (DCR): retain live cells for analysis
4. Single-cell RNA library using 10X Genomics platform (targeting 10,000 cells per sample)
5. Sequencing (4 samples on 1 S4-lane for 2,400 M reads, ~50,000-66,000 reads per cell)
6. Data analysis:
a. Generate clean data and trim reads and raw data quality check
b. Demultiplexing
c. Quality control
d. Sequence alignment
e. Quantifying expression levels of each cell
f. Clustering of single cells to identify subpopulations using t-SNE and UMAP plots
g. Aggregate analysis: pooled samples for differential gene expression analysis under the given sub-population
7. Turnaround Time: 15 working days for library preparation 18 working days for sequencing 5 working days for data analysis
8. Raw data and analysis results will be delivered through FTP
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