Statement of Specifications for the Liquid Chromatography-Mass Spectrometer.docx

Statement of Specifications for the Liquid Chromatography-Mass Spectrometer (LC-MS)

· The full system (LC, MS, Software, etc.) is tested together as part of the manufacturing process. This testing is to improve the user experience by; single delivery consignment, short installation time, reduced system start up failures, application specific install test to reduce time to become operational, and ready-made acquisition and processing methods.

· The MS must include a single piece electrospray capillary, which connects the integrated divert valve to the ACQUITY RDa ion source. This helps to minimize post-column dispersion to maintain high efficiency of the UPLC separation. The single piece electrospray capillary plumbs in just like PEEK tubing into a UV or FLR detector.

· The MS must include a pre-optimized ionization source which requires no physical user-adjustment for consistent performance.

· The MS must continuously monitor the performance of the instrument to guarantee quality data. These checks must include sensitivity and mass accuracy.

· The system must automatically provide the users with targeted guidance to resolve specific issues from within the software, removing the need for a user to search for solutions to the issues.

· The MS must have an integrated leak sensor that is user replaceable.

· The analyst must be automatically prompted to run a calibration when required.

· The MS must automatically apply the correct calibration for the selected mode and mass range defined in the analytical method.

· The MS must be able to automatically perform a system set-up incorporating beam check, detector set-up, analyzer tuning, calibration and lockmass setup, following a single click by the analyst within the software.

· The MS must be benchtop in design, having a footprint no bigger than 75.3 (H) x 40.0 (W) x 76.4 (D) cm (H x W x D).

· The total system dwell volume, as measured at the 50% point in a step gradient, must be ≤ 100 μL.

· The pump must be a binary pump designed for ultra performance liquid chromatography, designed to blend 2 (two) solvents and to select between four solvents.

· The pump must have an integrated degasser, which operates up to the maximum flow rate, with an independent channel for each solvent. The degasser must support additional channels for the sample manager. The total solvent volume must be less than 480 µL.

· The pump flow must be settable from 0.001 - 2,000 μL/min, in 1 μL/min increments. The pump flow accuracy must be less than or equal to ± 1.0% at 0.5 to 2.0 mL/min using 100% Solvent A, and the pump flow precision must be ≤ 0.075% RSD or 0.01min SD, whichever is greater.

· The fluorescence detector must have a standard analytical flow cell with a volume of not more than 2.0 µL for optimal peak dispersion of ultra performance liquid chromatography separations.

· The fluorescence detector must have a means to optimize lamp performance automatically and without user intervention. This compensates for lamp degradation over time without the need to frequently replace the lamp.

· The fluorescence detector flow cells must be simple to replace in a cartridge form with finger-tight fittings.

· The fluorescence detector must have a wavelength range of 200 to 890 nm for the excitation wavelength and 210 to 900 nm for the emission wavelength.

· The fluorescence detector must have an optical bandwidth of 20 nm or less.

· The fluorescence detector must be able to collect 2D data, 3D data and exλ-emλ plots.