Statement of Need.pdf

STATEMENT OF NEED (SON)

1.0 TITLE

STEDYCON STED & Confocal Microscope

2.0 BACKGROUND

The Fluorescence Microscopy and Imaging Center (FMIC) is a core facility for use by employees of the National Institute of Environmental Health Sciences (NIEHS). The FMIC provides researchers access to a variety of techniques that push the envelope on 3D cellular imaging. Most of the techniques are centered on obtaining digitized images of fluorescent cells from a confocal microscope. This STED system will greatly impact NIEHS because currently the FMIC has limitations on the resolution of the 3D cellular images that can be acquired in the core.

TYPE OF ORDER

This is a:

• Firm Fixed-Price Purchase Order

• Severable

3.0 PRODUCT FEATURES/SALIENT CHARACTERISTICS

Fragmentation-based molecular annotations are the gold standard in high-confidence biomarker identification using mass spectrometry. The new imaging parallel reaction monitoring parallel accumulation serial fragmentation (iprm-PASEF) workflow integrates CCS-enabled MALDI Imaging of lipids with a targeted MS/MS imaging approach that allows spatial fragmentation and analysis of multiple targets in a single acquisition. The workflow includes a fully integrated pipeline within our software ecosystem to generate accurate identifications with spatial fidelity.

The following product features/characteristics are required for this requirement:

STED and confocal optics:

• Excitation laser lines: 405nm (cw), 488nm, 561nm, 640nm (all pulsed)

• STED laser: 775nm (pulsed)

• Superior STED resolution: <40nm, typically 30nm (lateral, depending on dyes and objectives used)

• Based on easySTED: all laser lines aligned by design; maintenance-free optical beam path

QUAD-beam scanner:

• Scan lens-free design for minimal wavefront distortions

• Field of view: up to 90ìm x 80ìm (with 100x objective, may be reduced for STED by lateral chromatic aberrations of the microscope optics)

• Up to 1.1 frames/s @ 512x512 pixels; line frequency up to 800Hz

• Quasi-simultaneous line-interleaved scanning

Detection:

Three STED and four confocal imaging channels

STATEMENT OF NEED (SON)

• Four single-photon-counting APD modules; extremely low dark counts to render the finest details and superior photon detection sensitivity (>62 % typical)

• Detection bands: 420-480nm, 505-550nm, 575-625nm, 650-700nm

• Frame-interleaved, Line-interleaved or pixel-interleaved (confocal only) detection

• DynamicPlus detection

• Highly accurate time-gating for confocal and STED

• Motorized pinhole with 12 pinholes (10μm to 200μm)

Software:

• Browser-based control software operational on any modern PC, Mac or tablet

• Simple definition of z-stacks, acquisition queues and time series, auto-save function

Signal I/O:

• Detector signals and laser-pulse sync outputs available on the back panel without signal attenuation

(e.g. for use with additional TCSPC hardware)

• Frame-, line-, and pixel-sync output signals and a trigger input for synchronization with external hardware

Dimensions:

• Scan Unit: 11cm x 20cm x 20cm (BxHxT)

• Controlling Unit: 22cm x 55cm x 60cm (BxHxT)

• Total weight < 40kg (head and controlling unit)

• Continuous Laser-based hardware autofocus

• Sample Piezo Z-scanner

• FLIM hardware and software

• Active vibration isolation system

• Laser safety pack

• Objective Lens Zeiss alpha plan-apochromat

• Multipostion Imaging Package

• Training

• Installation

3.1 DELIVERY / INSTALLATION

Erica Scappini

National Institutes of Health National Inst of Environmental Health Sciences

111 TW Alexander Dr

Research Triangle Park

Durham, North Carolina, 27709

Items shall be delivered within 10 weeks of purchase order award.

4.3 TRAINING

• One day training