SSN LB 75N95023Q00313.pdf

Sources Sought Notice

1. SOLICITATION NUMBER:

75N95023Q00313

2. TITLE:

Ti2-E microscope with TIRF adapter, software, and component parts

3. CLASSIFICATION CODE:

6640 - Laboratory Equipment and Supplies

4. NAICS CODE:

334516 - Analytical Laboratory Instrument Manufacturing

5. RESPONSE DATE:

May 19, 2023, at 3:00 pm EST

6. PRIMARY POINT OF CONTACT:

Llakim Dubroff, Contract Specialist Llakim.Dubroff@nih.gov Phone: (301) 443-6677

7. DESCRIPTION:

This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement.

This notice is issued to help determine the availability of qualified companies under the NAICS Code 334516 - Analytical Laboratory Instrument Manufacturing, technically capable of meeting the Government requirement, to determine the method of acquisition, and availability of domestic sources manufactured in the United States in sufficient and reasonably available commercial quantities and of a satisfactory quality. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice.

The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. Small businesses are encouraged to respond.

For equipment/supply requirements, responses must include the place of manufacturing (i.e.

address if supply/equipment is a domestic end product and include country of manufacture). For equipment/supply requirements, small businesses must also address the size status of the manufacturer under the applicable NAICS code (i.e. address Non-Manufacturer Rule).

mailto:Llakim.Dubroff@nih.gov mailto:Llakim.Dubroff@nih.gov

Statement of Need and Purpose:

The National Institute on Neurological Disorders and Stroke (NINDS) is a part of the National Institutes of Health (NIH) and conducts research into the causes, treatment, and prevention of neurological disorders and stroke. The NINDS Cell Biology and Biophysics unit (CBBU) performs measurements of microtubules and microtubule associated proteins and their kinetics and dynamics at the single molecule level. To discern the molecular level events, images of the microtubules are required in a label free manner while observing the interacting proteins with fluorescent tags. Therefore NINDS requires a brand name or equal Ti2-E microscope with TIRF adapter, TIRF objective, Elements Control Software, LED light source, XY Stage and a Fusion BT camera to replace a system that was damaged in a recent flood. The acquisition of this brand name or equal system and component parts are in necessary in order for the CBBU to perform IRM and simultaneous fluorescence measurement on microtubules and microtubule associated-protein (MAP)s.

Purchase Description: A Capable Offeror must demonstrate its ability to provide to the Government the below listed microscope body and components including software and install/integrate the same with an instruments-warranty. A Capable Offeror must also demonstrate its ability to provide for the below described microscope body and components including software, a mechanical and electric parts and labor warranty with a minimum duration of one year from the date of installation. The microscope body and components listed in the table below must be brand name or equal:

1. Ti2-E Microscope

Qty. Product no. Description

1 MEA54000 Ti2-E Inverted Motorized Microscope Stand, Motorized Light Distribution; Eye 100/Left 100/Right 100/Left 80/ Eye Prisms, Sideport FOV 22mm C-Mount or 25mm F-Mount, Manual Switching Intermediate Magnification Lens with Status Detection 1.0-1.5x (1.5x exchangeable to 2.0x), Manual In/Out and Manual Focus Bertrand Lens with Status Detection, Built-In Hub for Motorized Accessory Connection, Includes 2 ISO C-Mount Sideport Adapters, Main Cable, Cable Clips and Toolkit

1 MEF55037 Ti2-E Controller, Provides Power for Motorized Stand and Motorized Components, USB and LAN Connection, Two Connectors Accommodating Four I/O Channels Each

1 MXA22149 S-TI2-EXT External Trigger Cable 2 97050 USB 2.0 A-B 15ft Cable 1 79035 Power Cord 120V, Lead Free, UL Listed, Length 7.5 Feet 1 MEB55855 Ti2-T-BC Binocular Body Base Unit with Side Photo Port 1 MEB52520 TC-T-ER Ergo Binocular Tube 2 MAK10110 CFI 10X Eyepiece F.N. 22mm Diopter Adjustment-NC 1 MEP59394 Ti2-ND-P Perfect Focus System 4, 340nm-800nm, Six Place DIC

Motorized Nosepiece with Motorized Reflector, Measure Unit, 45mm IR Block Filter for Diascopic Illumination, Pinch Guard and Drip Hose

1 MEE59925 Ti2-D-PD Diascopic Illumination Pillar, Tilt-Back Design with LockingMechanism, Centerable Condenser carrier, Filter Holder for Two Fixed45mm Filters

1 MEE55705 Ti2-D-LHLED Transmitted Light LED Lamphouse, Built-In Fly Eye Lens forWide, Evem Illumination, 45mm Neutral Density ND32 Filter for Brightfield Observation

1 MBN21816 Filter 45mm, ND16A Multi-Coated Neutral Density 1 MBN11200 Filter 45mm, GIF Green Interference (546/60nm) 1 MEV50010 Ti2-D-SF Diacopic Illumination Slider, Two Fixed 45mm Filter Positions and Two Sliders for Four 45mm Filters 1 MEL51920 Ti2-C-TC-E Motorized Condenser Turret, Seven Position, Aperture

Diaphragm, ND32 Filter In Holder, Light Blocking Module, Labels 1 MEL56200 T-C-LWD Lens Unit for System Condenser Turret, N.A. 0.52, Working

Distance 30mm 1 MEH51510 TC-C-ML-N1D LWD Dry DIC Condenser Module 1 MEH51520 TC-C-ML-N2D LWD Dry DIC Condenser Module 1 MEN51980 Ti-A Analyzer Cube for Transmitted DIC 1 MEN51944 Ti2-C-DICP-I Intelligent DIC Polarizer 2 MEV51030 Ti2-F-FLT-E Motorized Epi Fluorescence Turret, Six Position, Compatible with 25mm and 32mm Fluor Cubes 1 MEE54740 TI2-LA-FL-2 Epi-Fluorescence Module for D-LEDI Epi-Fluorescense

LED Illumination System on Ti2 Microscope. Allows for 25mm FOV.

1 MXA22205 C-FIBA Adapter for Fiber Light. Connects New Epi-FL Attachment

Module with D-LEDI Epi-Fluorescence LED Illumination System Connection to Fiber Type Light Source with 5mm Diameter Fiber; Intensilight.

1 MED54555 Ti2-FP Fixing Plate 1 MXA22152 Ti2-D-LSK Laser Safety kit (Required for Laser Safety Compliance) 1 MXA22158 S-Ti2I-LU Interlock Cable between Ti2-E, Ti2-A and LU-4A 1 77023046 Interlock Junction Box 1 91170 50/50 Mirror for L-APP Iluuminator (25.5 x36 x2mm) 1 MEE58017 TI-LA-Exchangeable Mirror (100% Reflect) 1 MEE54840 TI2-LA-BM-E Motorized Main Branch 1 MEE54880 TI2-LA-BS Sub Branch 1 MXA22144 TI2-LT-2X 2X Tube Lens 1 999-41 Service Installation of 2X Tube Lens

2. Computer and Software

Qty. Product no. Description

1 77023034 NI-BB PLUS with analog controller triggered device hub for directhardware triggering of optomechanical devices. Includes NationalInstruments PCIe 6323 NIDAQ card and trigger cable set.

Requiresappropriate hardware drivers and triggered device control modules forNIS-Elements.

1 77019779 Advanced Biosystems Z8 Workstation # Minimum specification based on HPZ8 featuring dual Xeon 3.8GHz CPUs, 128GB RAM, NVIDIA RTX A4000 GPU,512GB SSD OS drive, 1TB SSD data drive, 8TB data storage, 2x 10GBEthernet ports, 10x USB 3.1 ports Type A, DVD-RW, premium keyboard andoptical mouse. Windows 10 64-bit Professional. HP 3/3/3 warranty.

1 77012929 BenQ PD3205U DesignVue 31.5" 16:9 4K HDR FreeSync IPS Monitor 1 77019692 PC Accessory Kit, includes Surge Protector, USB to Serial Adapter, A-

Male to A-Female USB2.0 Extension Cable, 5 Port PCI Express USB 2.0Adapter Card.

1 MQS31000 NIS-Elements Advanced Research Package for 6D Image Acquisition and Analysis Includes Camera and Nikon Motorized Microscope Drivers (except Nikon RFA) Optional Modules Include: 6D Acquisition, Jobs, Image Database, 2D/3D Deconvolution, EDF, 2D Object Tracking,3D Measurement, Ratio & FRET, Object Classifier, General Analysis, STORM and SIM

4 MQS31410 NIS-Elements: Advanced Research Software Upgrade Agreement

(AR-SUA)

1 MQS43130 NIS-ELEMENTS JOBS ACQUISITION MODULE FOR CUSTOM

ROUTINES. AVAILABLE FORC AND AR ONLY. MQS42560 6D

IMAGING REQUIRED.

1 MQS41100 NIS-Elements: Hardware Module: Motorized Stage Control - device control for ASI, Ludl, Prior, and Maerzhauser stages.

1 MQS41220 NIS-Elements: Hardware Module: Wavelength control - device control for Sutter, Prior and Unibliz illumination devices including shutters and filter wheels.

1 MQS41320 NIS-Elements: Hardware Module: Motorized Focus Control - device control for external motorized focus control. Includes Prior, Maerzhauser, Nikon RFA, and PI Piezo Z drive (note that piezo is via RS232 only).

1 MQS42560 NIS-Elements: Module: 6D Imaging - Acquisition Module for 6 Dimensional Imaging, Includes Time, Channel or Wavelength and Focus, Multipoint or Multi Stage Position. Note that Device Drivers are Required, and only Available in AR Included in C and HC packages.

1 MQS42780 Device Control for Externally Triggering a Device Through a Camera *MQS41320 is a Required Module for Triggered Piezo Z *MQS41930 is a Required Module for Triggered DG4/5 Available for C, HC, AR & BR only

1 MQS42930 NIS-ELEMENTS ILLUMINATION SEQUENCE FOR FLEXIBLE

TRIGGERED LASER/LEDEXPERIMENT SETUP. AVAILABLE FOR C,

HC AND AR ONLY.

3. Objective

Qty. Product no. Description

1 MRD01995 CFI SR HP Apo TIRF 100x objective for STORM/SIM NA 1.49 OIL, WD 0.12mm,F.O.V. 22mm

4. TIRF Components

Qty. Product no. Description

1 97327 C-TIRF Ultra High Signal-to-Noise 405/488/561/638nm Quad TIRF Filter Set, Quad 405/488/561/638nm Laser Cleanup, HC TIRF 405/488/561/638nm Quad Dichroic Mirror in Metal Cube, HC Quad Barrier Filter and three Supplementary BP 525/50nm, 600/50nm and 700/75nm HC Laser Block Barrier Filters 25mm for Emission Filter Wheel. (6 filters total)

1 MEE59220 TI2-LA-TIRF-E Motorized TIRF Module for L-APP System 1 MXA22147 S-TI2-DCL Daisy Cable Long, Connects Filter Turrets and Manual L-

Apps Main Branches to Hub for Intelligent Mirror Position 1 MEE59178 TI2-LU-LSC Laser Safety Cover 1 MEE54405 Ti2-P-FWB-E Motorized Barrier Filter Wheel for Sideport, 7 Positions for either 25mm or 32mm Filters, C-Mount Included (50ms to adjacent positions)

5. Epi Light Source

Qty. Product no. Description

1 77060137 SOLA v-nIR Light Engine Gen III. 380-760nm unfiltered light output,control via USB or control pod (optional). Active output powerstabilization. Includes 3mm core LLG 2m with PVC outer sleeve, USBcable, power supply, and power cord. 24 month warranty.

1 MQS41920 Controls Shutter and Illumination Intensity from EXFO and SOLA (RS232slow Control) Light Sources Note: Control for Sutter Lambda XL, but MQS41220 also needed

1 96370 C-FLL Large Field of View DAPI Filter Set, High-Signal-Noise, Semrock Brightline®, Excitation: 356/30nm (341-371nm), Emission:

447/60nm (417- 477nm), Dichroic Mirror: 409nm

1 96372 C-FLL Large Field of View GFP/FITC/Cy2 Filter Set, High-Signal- Noise, Semrock Brightline®, Excitation: 466/40nm (446-486nm), Emission: 525/50nm (500-550nm), Dichroic Mirror: 495nm

1 96374 C-FLL Large Field of View DSRed/TRITC/Cy3 Filter Set, High-Signal- Noise, Semrock Brightline®, Excitation: 554/23nm (542.5-565.5nm), Emission: 609/54nm (582-636nm), Dichroic Mirror: 573nm

6. XY Stage

Qty. Product no. Description

1 77011833 3-Axis Stage controller for user w/Closed Loop DC Servo Motor stages and Z-Axis drive. Control electronics included ASI's provenantibacklash algorithm to increase bidirectional accuracy.

1 77020103 40TPI pitch lead screw flat top stage for Nikon Ti2 Microscope 1 77020104 2.5nm resolution linear encoders for flat top stage

7. Fusion BT

Qty. Product no. Description

1 77054116 High Speed interface kit Firebird CoXpress board and cables for ORCAFusion. Includes CoXpress board with full height bracket and two CANARECoXpress cables; one red and one black.

Salient characteristics: Capable Vendors should demonstrate their ability to provide NINDS the following brand name or equal characteristics:

• A Ti2-E microsocpe imaging system

• An XY Stage with 40 TPI lead screw and bidirectional backlash correction for precise positioning.

• An objective lens with an NA of 1.49

• A camera capable of reaching 26 fps at full framerate

• A combined EPI/TIRF adapter unit for Ti2 microscope

• A white light LED light source capable of manual or remote control

• A CPU capable of streaming full camera chip at least 30 fps

• An installation of Elements control software for hardware interfacing

• All required cables for proper connection

Period of Performance: NINDS requires a 1-Year warranty on all parts electrical and mechanical. In the event of a failure during the warranty period vendor shall provide a factory certified and trained technician to perform repair at no cost to the government. This includes travel, parts and labor during the warranty period.

Capability Statement /Information Sought:

Companies that believe they possess the capabilities to provide the required products or services should submit documentation of their ability to meet each of the project requirements to the Contracting Officer. The capability statement must specifically address each of the project requirements separately. Additionally, the capability statement should include 1) the total number of employees, 2) the professional qualifications of personnel as it relates to the requirements outlined, 3) any contractor GSA Schedule contracts and/or other government-wide acquisition contracts (GWACs) by which all of the requirements may be met, if applicable, and

4) any other information considered relevant to this program. Capability statements must also include the Company Name, Unique Entity ID from SAM.gov, Physical Address, and Point of Contact Information. The response must include the respondents’ technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses.

Interested companies are required to identify their type of business, applicable North American Industry Classification System (NAICS) Code, and size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice. However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number.

Submission Instructions:

The response is limited to ten (10) page limit. The 10-page limit does not include the cover page, executive summary, or references, if requested.

All responses to this notice must be submitted electronically to the Contracting Officer Identified below. The response must be received on or before May 29, 2023, at 3:00 pm EST. Facsimile responses are NOT accepted.

Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s qualifications to perform the work.

Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in www.sam.gov. However, responses to this notice will not be considered adequate responses to a solicitation.

Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).”

Closing Statement: THIS SYNOPSIS IS NOT A REQUEST FOR COMPETITIVE PROPOSALS. However, interested parties may identify their interest and capability to respond to this notice.

A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. All responsible sources may submit a capability statement, proposal, or quotation which shall be considered by the agency. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement.

Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Unique Entity ID (UEI), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.

All responses must be received by the closing date and time of this announcement and must reference the solicitation number, 75N95023Q00313. Responses must be submitted electronically to Llakim Dubroff, Contract Specialist, at Llakim.Dubroff@nih.gov U.S. Mail and Fax responses will not be accepted.

Primary Point of Contact:

Llakim Dubroff, Contract Specialist mailto:Llakim.Dubroff@nih.gov