Specifications.pdf
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- Attached to
- RNA Sequencing Federal contract opportunity
- Solicitation number
- 12305B24Q0173
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This document contains the Specifications for a federal contract opportunity related to RNA sequencing services. The USDA, ARS, Floral and Nursery Plant Research Unit in Beltsville, MD requires RNA sequencing services, including RNA purification/clean-up, Illumina RNA library preparation, Illumina RNA sequencing, and RNA sequencing data analysis. The requirements include 252 samples, 2x150bp sequencing, 50x genome coverage, and 50 million paired-end reads per sample. The services must comply with Section 508 accessibility standards. The vendor must provide documentation of quality checks and complete analysis results within 90 days after receiving the purchase order. The federal contract opportunity has a Solicitation Number of 12305B24Q0173 and is a 100% small business set-aside under NAICS code 541380 with a $19.0 million size standard. Quotes are due by 8/26/2024 at 2:00 PM PST and will be evaluated based on price and technical capability to meet the requirements.
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| File | Type | Posted |
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| Contract Terms and Conditions.docx | DOCX document | |
| Past Performance.docx | DOCX document | |
| Wage Determination BARC 2015-4281 Rev. 30- 07222024.pdf |
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SPECIFICATION – Boxood RNA sequencing
July 2, 2024
I BACKGROUND
The USDA, ARS, Floral and Nursery Plant Research Unit in Beltsville, MD requires RNA sequencing services, to include: RNA QC, Illumina RNA library preparation with QC Illumina RNA sequencing, and data analysis.
This sequencing data will be used for ongoing research in developing disease resistance in boxwood varieties.
II. REQUIREMENTS.
• RNA purification/clean-up with QC and quality report. Quantity - 252 samples
• Illumina NovaSeq RNA Library preparation with QC and size selection. Quantity - 252 samples
• Illumina NovaSeq RNA sequencing, 2x150bp, 50x genome coverage per sample, 50 million paired-end reads per sample. Quantity - 252 samples
• RNA sequencing data analysis package including differential expression, trimming, mapping, alternative splicing, and gene ontology.
• FASTQ files delivered to ARS
• Total RNA on dry ice will be provided to the vendor.
• PolyA selection
• Annotated Buxus sinica ‘Wintergreen’ (see the 2022 paper by Chanderbali et al. in Nature Communications).
III. Section 508 Accessibility Compliance
It is mandatory that data generated through this Contract meet the applicable accessibility standards at 36 CFR 1194, unless an agency exception to this requirement exists. (36 CFR 1194 implements Section 508 of the Rehabilitation Act of 1973, as amended, and are viewable at: http://www.section508.gov/, then go to 508 Law.
IV. Delivery
The Contractor shall provide documentation of quality checks on RNA, library, FASTQ sequencing files, and complete analysis results to the USDA, ARS, USNA, Floral and Nursery Plant Research Unit, 10300 Baltimore Ave, Beltsville, MD 20705 within ninety (90) days after the receipt of the purchase order. Data
V. Contact: Fred Gouker – Fred.Gouker@usda.gov
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