Specifications.docx

Specifications:

Rawlins eDNA Inventory and Sampling – Backpack Sampler Introduction

This proposal is part of a multi-year project for the surveying and subsequent monitoring of aquatic and semi-aquatic wildlife located within the water systems of the Rawlins Field Office utilizing eDNA sampling. This project will improve our knowledge of local aquatic habitat and the species utilizing it, allowing for more effective habitat management. eDNA, or environmental DNA, is the DNA found as cellular material introduced into the environment by an organism, generally through skin cells, gametes or excrement. eDNA can persist in the environment for a significant period of time and can be detected, even in trace amounts, using specialized sampling equipment. Utilizing eDNA sampling technologies and subsequent analysis, RFO biologists will be able to detect a range of species and determine distribution and diversity across the vast number of perennial water systems located within the Field Office’s boundaries, including cryptic species not easily identified through traditional survey methods. This surveying technique will allow for a more efficient sampling of our aquatic habitat, allowing for a larger amount of areas to be surveyed in a single field season as compared to traditional methods.

This proposal is for the acquisition of the sampling equipment necessary to perform the above-described work.

Work Site Location

BLM-managed aquatic systems within the Rawlins Field Office jurisdiction. These systems may be lotic or lentic; perennial, ephemeral or intermittent.

Deliverables

The awarded shall be able to meet specifications outlined in the below specifications including battery type (lithium ion), backpack sampling equipment and self-preserving sample filters consistent with US Patents 11307118 and 11471837, these unique specifications allowing for intact data retrieval and global positioning records despite varying project timeframes and fluctuations in local conditions such as temperature, weather, remoteness and accessibility. The awarded should also be able to provide an alternative low-cost sampling unit (that still utilizes the above described self-preserving filters: US Patent 11471837).

Project Contacts

TBD

Delivery:

Rawlins BLM Field Office c/o John Martin 1300 North 3rd St.

Rawlins WY, 82301 307-328-4370 (office Ph#)

Period of Performance (i.e., expected delivery by): 11/15/2024 or earlier.

Equipment description(s), Quantities and Specification(s) for contract award:

Item 1: eDNA Sampler Backpack Combo (Lithium-Ion Battery) Quantity: 1 Description: Lithium-Ion battery powered eDNA Sampler backpack combo including a pack frame, remote control with charger, tubing, battery, and charger. Onboard GPS that will automatically geotag samples and onboard computer that completes data logs for every sample event in .csv files accessible via USB. System capable of mobile DNA filtration.

Specification:

Sensors

Pump

Communications

Batteries

Size and Weight

Width: 16.4 in. (41.65 cm) Depth: 15 in. (38.1 cm) Weight: 24 lb. (10.89 kg) without battery

Width: 2.5 in. (6.35 cm) Depth: 0.94 in. (2.39 cm) Weight: 0.4 lb. (181.4 g)

Item 2: eDNA Citizen Scientist Sampler (Lithium Ion Battery) – Specification/Included Quantity: 1 Description: Citizen Scientist Sampler with battery, charger, hose kit, and vacuum bottle. Sampling system needs to be compact, easy to ship, and has everything you need to quickly collect high-quality samples.

Sampler should include:

· eDNA Citizen Scientist Sampler

· Lithium Battery

· Battery Charger

· Hose Kit

· 2 Liter Vacuum Bottle

Item 3: eDNA Sampler Transect Pole Quantity: 1 Description: eDNA Sampler Trident pole should be designed for mobile eDNA sampling. Three filter clamps allow for triplicate eDNA sampling while moving along a transect. The pole should come with a mount for the eDNA Sampler remote control and breaks down into two parts for easy transportation.

Specifications:

Length: Extended

6.6 ft

Item 4: Extra Lithium Battery (for sampler) Quantity: 2 Description: 12V 11.1Ah lithium iron phosphate battery for eDNA Sampler.

Specifications:

Item 5: eDNA Self-Preserving Filter Packs (Pore Size 5µm) Quantity: 50 Description: Preloaded, openable inline filter housing w/ tubing extension for filter housing. Uses a biodegradable/hydrophilic resin to absorb moisture from the filter membrane, and automatically preserve the captured eDNA.

Specifications: