SOW Equip Milliplex FlexMAP 3D - betasam.docx

STATEMENT OF WORK (SOW)

Introduction and Overview Chief of Neurology Service at VA New Jersey Health Care System (VANJHCS) in VISN2 is requesting BRAND NAME purchase of a Millipore Milliplex FlexMAP 3D vendors of a multiplex immunoassay system utilizing the Luminex technology satisfying these product specifications below.

Background The purpose of this Statement of Work is to purchase a multiplex immunoassay system utilizing the Luminex technology. The Neurology Service at VANJHCS has been carrying out preclinical research in traumatic brain injury, neuroinflammatory disorders, and neurodegenerative disorders with a goal of eventually improve health of veterans with these disorders. To carry out experiments detect simultaneously a large number of proteins in a single biological sample to assess changes the brains in these disorders and treatment effects, one needs a Luminex xMap technology, a bead-based multiplexed immunoassay system on a microplate format.

Objectives The objective of this SOW is to purchase a multiplex immunoassay system utilizing the Luminex technology to be used for sensitive molecular and cellular neurobiology experiments under a firm fixed-price contract.

Period of Performance Vendor will deliver within (90) days after receipt of order.

Place of Performance / Place of Delivery VA New Jersey Health Care System Attn: Logistics Receiving Warehouse Building 1 B-Level 385 Tremont Ave East Orange NJ 07018

Product Specifications

· To assess a large number of analytes in a single biological sample, one requires Luminex technology.

· FlexMAP 3D is Luminex’s most advanced and versatile multiplex platform assessing up to 500 analytes through the combination of differentially dyed fluorescent microsphere sets.

· Higher Throughput: Dual sample fluidics paths and increased sample injection speed result in a faster sampling rate. The FLEXMAP 3D System can process 384 samples and generate up to 144,000 data points in just over an hour.

· High Plexing Capability: The FLEXMAP 3D is compatible with magnetic and non-magnetic microspheres and can discriminate up to 500 different microsphere sets. The platform offers a large menu of commercial kits and reagents to develop custom assays for a wide variety of applications including nucleic acid assays, immunoassays, receptor ligand assays, and enzymatic assays.

· Automation/LIS Compatibility: xPONENT® Software controls the FLEXMAP 3D System and offers interfacing options for Laboratory Information Systems (LIS) and front-end automation platforms. The system accommodates 96 and 384-well plate formats for enhanced sample flexibility and increased throughput.

· Reliability: The FLEXMAP 3D System has multiple features allowing walk away start-up, shut-down, and maintenance operations, and greatly enhances usability for High Throughput Screening (HTS) applications.

· Key Features: Multiplexing: Up to 500 analytes in a single sample. Dynamic Range (Typical): ≥4.5 logs. Read Time: 96-well plate in ~20 min (up to 144,000 tests/hour); 384-well plate in ~75 min (up to 153,600 tests/hour). Daily Start-Up/Shut-Down: ~30 min.

· MILLIPLEX MAP kits has the broadest selection of analytically validated protein analytes across a wide range using the Luminex technology.

· MILLIPLEX Belysa analysis software package provides user-friendly options to facilitate the analysis of the data acquired using FlexMAP 3D.

Security Not applicable. This multiplex immunoassay system will not interface with EOVA Intranet. Certification & Accreditation (C&A) requirements do not apply, and a Security Accreditation Package is not required.