Sources Sought Notice_Chromium Single Cell Sequencing Kits.pdf

SOURCES SOUGHT NOTICE

1. Solicitation Number: 75N95022Q00374SSN

2. Title: Chromium Single Cell Sequencing Kits

3. Classification Code: 6640 Laboratory equipment and supplies

4. NAICS Code: 334516 - Analytical Laboratory Instrument Manufacturing

5. Size Standard: 1,000 employees

6. Description:

This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations.

The purpose of this notice is to obtain information regarding the availability and capability of qualified business sources; whether they are small businesses or other than small businesses. For equipment/supply requirements, responses must include the place of manufacturing (i.e. state if supply/equipment is a domestic end product and include country of manufacture). For equipment/supply requirements, small businesses must also provide the size status of the manufacturer under the applicable NAICS code (i.e. address the Non-Manufacturer Rule).

Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. This notice is issued to help determine the availability of qualified vendors technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes.

No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only.

This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement.

This notice is issued to help determine the availability of qualified vendors technically capable of meeting the Government requirement, to determine the method of acquisition, and the availability of domestic products manufactured in the United States in sufficient and reasonably available commercial quantities and of a satisfactory quality.

It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes.

No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. Small businesses are encouraged to respond.

Purpose and Objectives: The National Institute on Neurological Disorders and Stroke (NINDS) is a part of the National Institutes of Health (NIH) and conducts research into the causes, treatment, and prevention of neurological disorders and stroke. The NINDS is conducting a large-scale, single cell, RNA study, and requires chromium single cell sequencing kits and a library construction kit for sequencing of single cells plus enriched immune cell profiles to explore the T cell receptor, B cell receptor, and transcriptional changes associated with HIV disease status.

Project requirements: A Capable Offeror must demonstrate its ability to provide the following brand name or equal items to the Government:

Quantity Description – Brand name or Equal 8 Product Code 1000263 - Chromium Next GEM Single Cell 5’ Library and

Gel Bead Kit v2 (dual index), 16 rxns 10 Product Code 1000286 - Chromium Next GEM Chip K Single Cell Kit, 48 rxns 4 Product Code 1000215 - Dual Index Kit TT Set A 96 rxns 15 Product Code 1000190 - Chromium Single Cell 5’ Library Construction

Kit, 16 rxns 8 Product Code 1000253 - Chromium Single Cell Human BCR Amplification

Kit, 16 rxns 8 Product Code 1000252 - Chromium Single Cell Human TCR Amplification

Kit, 16 rxns

A Capable Offerors must demonstrate its ability to provide following brand name or equal items with the below stated Salient Characteristics:

1. Chromium Next GEM Single Cell 5’ Library and Gel Bead Kit v2 (dual index), 16 rxns (quantity: 8) Salient characteristics:

• The kit must identify and characterize rare cell types.

• The kit must analyze and understand cellular heterogeneity and how this contributes to the biological system.

• The kit must perform cellular phenotyping with single cell RNA-seq to identify novel targets, biomarkers, and cell types and states without use of pre-selected targets.

• The kit must evaluate mRNA and cell surface protein expression profiles within the same cell.

• The kit must perform high-throughput and high-resolution functional genetic screens in tens of thousands of cells simultaneously.

• The kit must assess comprehensive gene expression phenotypes.

• The kit’s shelf life must be 6 to 12 months.

2. Chromium Next GEM Chip K Single Cell Kit, 48 rxns (quantity: 10)

Salient characteristics:

• Able to generate and recover up to 10,000 cells in each GEM well

• Able to generate droplets in 8 parallel channels to ensure minimal batch effect that might cloud biological reading.

• Must have a gasket cover that is aligned with the chip where microfluidic flow is generated

3. Dual Index Kit TT Set A 96 rxns (quantity: 4)

• Able to de-multiplex multiple samples in the same run of RNA-sequencing pipeline

• Able to add i7 sample indexes to each read

4. Chromium Single Cell 5’ Library Construction Kit, 16 (quantity: 15)

• Includes fragmentation buffer, fragmentation enzyme, lligation bugger, DNA ligase, adaptor oligs, amplification mis, SI-PCR primer

• Able to generate T and B cell enriched cDNA compatible with next generation sequencing machines

5. Chromium Single Cell Human BCR Amplification Kit, 16 rxns (quantity: 8)

• Must be able to enrich cDNA libraries generated from the Chromium Next

GEM Single Cell 5’ Library and Gel Bead Kit v2 for B cell receptor sequences

6. Chromium Single Cell Human TCR Amplification Kit, 16 rxns (quantity: 8)

• Must be able to enrich cDNA libraries generated from the Chromium Next GEM Single Cell 5’ Library and Gel Bead Kit v2 for T cell receptor sequences

In case domestic sources are available and capable of fulfilling this requirement, and a future solicitation is published, the Government will use evaluation preferences in accordance with FAR Part 25.

Capability statement /information sought. Vendors that believe they possess the capabilities to provide the required products should submit documentation of their ability to meet each of the project requirements to the Contracting Officer. The capability statement must specifically address each of the project requirements separately.

Additionally, the capability statement should include 1) the total number of employees, 2) the professional qualifications of personnel as it relates to the requirements outlined, 3) any contractor GSA Schedule contracts and/or other government-wide acquisition contracts (GWACs) by which all of the requirements may be met, if applicable, and 4) any other information considered relevant to this program. Capability statements must also include the Company Name, Unique Entity ID from SAM.gov, Physical Address, and Point of Contact Information. The response must include the respondents’ technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses.

For equipment/supply requirements, responses must include the place of manufacturing (i.e. address if supply/equipment is a domestic end product and include country of manufacture). For equipment/supply requirements, small businesses must also address the size status of the manufacturer under the applicable NAICS code (i.e. address Non- Manufacturer Rule).

Interested companies are required to identify their type of business, applicable North American Industry Classification System (NAICS) Code, and size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice.

However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number.

The information submitted must be in and outline format that addresses each of the elements of the requirement and in the capability statement /information sought paragraphs stated herein. A cover page and an executive summary may be included but is not required.

All responses to this notice must be submitted electronically to the Contracting Officer identified below. Fax and mail responses are NOT accepted. The response must be submitted to Marlene Milgram, Contracting Officer, at e-mail address marlene.milgram@nih.gov. All responses should include the solicitation number and title in the subject line.

The response must be received on or before July 26, 2022 at 6:00 PM Eastern Time.

mailto:marlene.milgram@nih.gov

“Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s qualifications to perform the work.

Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in www.sam.gov. However, responses to this notice will not be considered adequate responses to a solicitation.

Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).”