Sources Sought.pdf

SOURCES SOUGHT NOTICE

1. Solicitation Number: 75N92020Q0085

2. Title: Florescence Activated Cell Sorting System

3. Classification Code/Title: 6640

4. NAICS Code/Title: 334516

5. Description:

This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement.

This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only.

Background:

Research in the NIDCR Intramural Research Program revolves around five main research areas: Craniofacial Developmental, Cell and Matrix Biology; Epithelial and Salivary Gland Biology and Dysfunction; Immunology and Inflammation; Sensory Biology; and Skeletal Biology. Within these research areas, our scientists are tasked with elucidating the molecular mechanisms that underlie a plethora of biological processes as they relate to both basic research and disease manifestations in a clinical setting.

The Combined Technical Research Core (CTRC) provides essential Flow Cytometry analysis and Florescence Activated Cell Sorting (FACS) services to the entire NIDCR/DIR and its collaborators.

Purpose and Objectives:

The laboratory requires a high dimensional FACS system for single cell isolation.

Project Requirements:

The instrument shall accommodate high parameter cell sorting demands and safe single cell sigh-content high-parameter cellular including human cell sorting capabilities. The instrument shall be a FACSymphony S6 High Parameter SORP cell sorter or equivalent, a bench-top high speed cell sorter equipped with five lasers (355, 405, 488, 561, and 637nm) capable of analyzing up to 30 different colors plus forward and side scatter. The instrument shall include the following or equivalent:

Product Number Description UOM QTY

662937 00382906629371

FACSymphony S6 Special Order System

EA 1

340345 00382903403455

BD FACS Clean Solution 5L EA 1

345249 00382903452491

Accudrop Fluorescent Beads 25 Tests

EA 1

340486 00382903404865

Calibrite 3 Color Beads Kit 25 Tests IVD

EA 1

340487 00382903404872

Calibrite APC Beads 25 Tests IVD

EA 1

556291 00382905562914

Rainbow Flrs Prtcl Bright 3.0-3.4um 5mL

EA 1

655050 00382906550507

BD FACSDiva CS&T Research Beads Kit 50T

EA 3

99-30211-00 BD FACSflow Sheath Fluid 20L

EA 2

625838 00382906258380

HIGH PARAMETER

IMMUNOLOGY

PANEL

EA 1

657590 00382906575906

Option Hood Safety Biological

EA 1

643154 00382906431547

Aria II Temperature Control Option 115V

EA 1

566352 00382905663529

HPS Fluorochrome Evaluation Kit

EA 1

624371 00382906243713

HPS Prototype Fluorochrome Eval Kit

EA 1

660360 00382906603609

Compressor Air EXT120V/60HZ 0-120Psi 4L

EA 1

The system shall meet these hardware specifications:

(A) The capability of up to 9 lasers, 60 parameters with 58 colors.

(B) State of the art fiber optics to carry the light/fluorescent signal from the flow cell to the photomultiplier tube detectors (PMTs.)

(C) The option of tube, APD or Silicon Chip detectors based on maximum channel sensitivity with each type.

(D) Decagon optical arrangement for lasers.

(E) Air-cooled lasers.

(F) Fixed alignment optics in conjunction with solid state lasers ranging in power from 60mW to 1000mW.

(G) Easily replaceable optical filters and mirrors for experimental flexibility.

(H) No special room requirements (no special electrical requirements, no special plumbing requirements, and no special air requirements).

(I) Sensitivity of <15 MESF-PE and <50 MESF-FITC.

(J) Provide for performing real-time statistics via the DIVA software.

(K) Class II Biosafety cabinet with integrated safety interlocks.

(L) Quantiflash LED Integrated Pulser QC tool.

(M) Capability of analyzing at least 30 logical gates.

(N) State of the art low noise digital acquisition electronics with acquisition speeds of

40000 events per second.

(O) Six-way high speed sorting and single cell deposition into 96 and 384well microtiter plates

The system shall meet these software specifications:

(A) Capability of integrated Laser Control Software which allows the user to adjust laser power up or down on an as needed basis.

Other specifications:

(A) Shall be compatible with the existing FACS Analyzer BD Symphony for transition of experimental protocols and templates.

(B) Shall be software compatible with existing laboratory (BD Diva) and institute high parameter cytometers for ease of transition of existing experimental protocols and minimum operator training.

(C) Shall be compatible to permanently be integrated in a Baker Class II Type A2 biosafety hood.

Other Requirements

• Shall provide user training.

• Shall have a proven user base with service support guaranteed within 48 hours.

Delivery

• The Contractor shall deliver and install the equipment within 90-120 days after receipt of order. Delivery must be Freight on Board (FOB) Destination and include inside delivery. On-site training must take place within four (4) weeks of equipment installation. The equipment shall be delivered and installed in coordination with the Contracting Officer’s Representative (COR). The contractor shall provide an Original Equipment Manufacturer (OEM) certified service technician to perform setup, installation, and an on-site training session for staff members.

Warranty

• The Contractor shall warrant that the all equipment will be free from defects for a period of at least twelve (12) months from the date of installation, inspection by the Government, and acceptance.

Capability statement /information sought.

The respondent must also provide their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements.

One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2” x 11” paper size, with 1” top, bottom, left and right margins, and with single or double spacing.

The information submitted must be must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein. A cover page and an executive summary may be included but is not required.

The response is limited to ten (10) page limit. The 10-page limit does not include the cover page, executive summary, or references, if requested.

The response must include the respondents’ technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses.

All responses to this notice must be submitted electronically to the Contract Specialist.

The response must be submitted to Natalie Bruning at e-mail address natalie.bruning@nih.gov.

The response must be received on 05/25/2020 by 12:00 PM Eastern Time.

Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s qualifications to perform the work.

Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in betaSAM. However, responses to this notice will not be considered adequate responses to a solicitation.

Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).