Sequencer Requirements.docx
DOCX document 12 KB Posted
- Attached to
- NextSeq 2000 Sequencer Federal contract opportunity
- Solicitation number
- RFQ-NIAID-2031537
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Provision 52.204-26 Dec 2019.pdf | ||
| FAR 52.204-24 Provision Dec 2019.docx | DOCX document |
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Text version
Sequencer Specifications: (Must be compatible with current Illumina NextSeq 550 Sequencer) Small whole-genome sequencing (300 cycles) 130 Mb genome; > 30× coverage 30 samples ~29 hours 75 samples ~48 hours
Whole-exome sequencing (200 cycles) 50× mean targeted coverage; 90% targeted coverage at 20× 16 samples ~21 hours 40 samples ~33 hours Single-cell RNA-Seq (100 cycles) 4K cells, 50K reads/cell 2 samples ~13 hours 5 samples ~19 hours
Output per flow cell 2 × 50 bp 40 Gb 100 Gb 2 × 100 bp 80 Gb 200 Gb 2 × 150 bp 120 Gb 300 Gb Quality scores 2 × 50 bp ≥ 85% of bases higher than Q30 2 × 100 bp ≥ 80% of bases higher than Q30 2 × 150 bp ≥ 75% of bases higher than Q30 Run time 2 × 50 bp ~13 hours ~19 hours 2 × 100 bp ~21 hours ~33 hours 2 × 150 bp ~29 hours ~48 hours
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