SAM_-_Pre-Solicitation__75N94024Q00213_1.pdf

75N94024Q00213

INTRODUCTION

THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A

CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION.

The National Institutes of Health, National Institute of Child Health and Development (NICHD) intends to award a purchase order without providing for full and open competition to 10X GENOMICS, INC. for Single Cell RNA Sequencing Kits in support of NICHD Thyroid Hormone Studies.

BACKGROUND

Thyroid hormone (TH) is critical for human development. To study how genes in the TH signaling pathway function during vertebrate development, we use either transgenic or gene-knockout animals to analyzed gene expression and cell fate changes in different animal models, i.e. mouse intestinal development and frog intestinal remodeling during metamorphosis. Single Cell RNA Sequencing is an newly developed technology to advance such analyses at single cell resolutions, so we can get deeper understandings on how gene expression is regulated by TH signaling molecules within single cells. The Chromium GEM-X Single Cell 3’ Chip-based single cell RNA sequencing kits achieve such goals.

JUSTIFICATION

The Chromium GEM-X Single Cell RNA sequencing analysis from 10X Genomics uses GEM-X Gel Beads deliver assay-specific oligonucleotides into nanoliter-scale liquid/oil emulsion partitions for barcoding and amplification of target nucleic acid templates. With a library of up to 4,000,000 available barcodes, the technology delivers an extremely high level of resolution for single cell RNA sequences analysis. We have used such kits from the same vendor in our studies and generated interesting data to reveal the important molecular mechanism of mouse intestinal homeostasis.

We used such kits from the same vendor to further advance our studies, including to expand similar research into our project using frog metamorphosis as model, also produced very promising data revealing signature transcriptome changes during frog intestinal epithelial maturation at single cell resolution. We need to expand the current research using frog metamorphosis model to other organs and tissues, such as the whole intestine and liver, etc. To ensure consistence of the data generating and analysis, we need to order two new kits from the same vendor. The ChromiumTM technology is developed by 10X Genomics and all the related supplies are manufactured/sold exclusively by 10X Genomics. The NIH (including the NICHD MGC Sequencing Core Facility) has purchased multiple ChromiumTM Controller manufactured also by 10X Genomics to process single cell samples to perform single cell sequencing analysis using the reagents that we plan to purchase.

To our knowledge, alternative reagent kits would not be compatible with our current equipment.

NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE

The intended procurement is classified under NAICS code 325413 with a Size standards in number of employees: 1,250.

PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS

75N94024Q00213

REGULATORY AUTHORITY

The resultant contract will include all applicable provisions and clauses in effect through the Federal Acquisition Regulation (FAR) Circular (FAC) 2024-05 effective 05/22/2024. This acquisition is conducted under the procedures as prescribed in FAR subpart 13-Simplified Acquisition Procedures at an amount not exceeding the simplified acquisition threshold ($250,000) and FAR Subpart 12- Acquisition of Commercial Items.

STATUTORY AUTHORITY

This acquisition is conducted under the authority of FAR 13.106(b)(1) Soliciting from a single source - For purchases not exceeding the simplified acquisition threshold.

PLACE OF DELIVERY

NIH/NICHD

Bldg. 49 Room 6A83 Rockville Pike Bethesda, MD 20892

DESCRIPTION OF REQUIREMENT

Chromium GEM-X Single Cell 3' Kit v4, 16 rxns Chromium GEM-X Single Cell 3' Chip Kit v4, 4 chips Dual Index Kit TT Set A 96 rxns Shipping and Handling

CLOSING STATEMENT

This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability in response to this notice and may submit a capability statement, which shall be considered by the agency. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement.

A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement.

All respondents must have an active registration in the System for Award Management (SAM) www.sam.gov. All responses to this notice shall be submitted electronically by 12:00 PM Eastern Standard Time, on 8/12/2024 to jacob.balenson@nih.gov and shall include pre-solicitation # 75N94024Q00213 in the subject line.

http://www.sam.gov/ mailto:jacob.balenson@nih.gov