2-Brandname_JOFOC_for_posting.docx

Justification for Other than Full and Open Competition:

Brand Name at or Below the Simplified Acquisition Threshold

Project Title: Life Technologies QuantStudio6 Flex real-time PCR instrument

I. Identification of the agency and contracting activity Address of the requesting lab/office: 903 S 4th St; Hamilton, MT 59840

A. Project Background Laboratory of Virology scientists broadly study viral agents requiring high or maximum containment (biosafety level-2 to biosafety level-4) that cause viral hemorrhagic fevers, viral encephalitis, and certain respiratory diseases. These agents include filoviruses, bunyaviruses, arenaviruses (such as Lassa virus), flaviviruses, orthomyxoviruses and coronaviruses. This work employs investigations in cell culture; animal models, including nonhuman primates; reservoir species; and arthropod hosts in order to elucidate the viral pathogenesis, immune responses, and molecular evolution, cellular and molecular biology, and vector-host interactions.

B. Project Description Laboratory of Virolgy requires a real-time PCR instrument for analyses of transcriptional regulation and quantitation of virus infection in a variety of in vitro and in vivo models. The lab currently has an ABI HT7900 Real-time PCR instrument that is used by multiple researchers in LV and other RML laboratories. The new instrument is requested to replace the HT7900 that is obsolete and will no longer be serviced by ABI. The QuantStudio 6 Flex Real-Time PCR system has been selected because it will allow seamless transition of many assays that have been optimized using ABI technology and reagents.

C. Description of the supplies/services to be purchased

· QuantStudio6 Flex real-time PCR system, incl 384well block (interchangeable), Dell desktop, Quantstudio 6 and 7 software, primer express software, one-year parts & labor

· 384-well calibration install

· Real-time PCR on-site application training, full day

· Fast 96well block upgrade with heated cover, plate adaptor, tube adaptor, storage case, calibration kit

· Fast96well calibration install

· CREDIT for trade-in of HT7900 instrument

· Manufacturer: Life Technologies

II. Identification of the statutory authority permitting other than full and open competition (SELECT ONE)

☒ 41 USC 3304(a)(1) and FAR Part 6.302-1: Only one responsible source/Brand Name