Required Specifications for the Real Time PCR system 4.28.20.docx

Required Specifications for the Real-Time PCR System (96- well)

· Instrument is licensed for Real-time PCR.

· An adjustable touchscreen on the instrument that has angle of rotation 12-55˚ or better.

· Instrument can be run standalone, without an attached computer, and accessed via WiFi, ethernet, or USB 2.0 or newer.

· Comes with the complete comprehensive software (e.g., allelic discrimination, gene expression analysis, end-point analysis, capability to do many types of plots, and at least a t-test and ANOVA analyses), with free software updates, and free copies available for office computers. Designed to run on a Windows 10 computer. Data and images can be exported in multiple formats (not just one).

· Must be capable of firmware and software updates done on site by our IT technician.

· Network capable to save runs on the local server.

· Capable of emailing a completed run on the network.

· Capability to integrate with an already existing cloud computing platform.

· User can set up usernames for different scientists for improved organization.

· Five fluorescent dyes can be detected in a single reaction well and has one dedicated FRET channel.

· The optical detection consists of at least six filtered LEDs for excitation and at least six filtered photodiodes for d1etection with a range of excitation and emission at least 450-730 nm.

· Instrument has a dynamic range of 10 orders of magnitude or better.

· Lid automatically adjusts pressure over reaction wells, and lid automatically opens and closes.

· An operational gradient range of at least 30-100 ˚°C with a programmable range of at least 1-24 ˚°C.

· Peltier heating and cooling; lid heats up to 110 ˚°C.

· User can view real-time data on the thermal cycler base.

· Temperature: Range – 4-100 °C or better. Accuracy – better than ± 0.2 °C at 90 °C. Uniformity – better than ± 0.3 °C, well-to-well, within 10 seconds or less of reaching 90 °C.

· Scan time must be 12 seconds or better (all channels), but for a single channel must be 3 seconds or better.

· 96-well fixed block with reaction volumes per well ranging from 1-50 microliters.

· Must fit in a space that is 22”x15”x15”.