QPCRS_specs_1.docx

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Attached to
3 QPCR SYSTEMS Federal contract opportunity
Solicitation number
140G0325Q0091
Issued by
Department of the Interior US Geological Survey Office of Acquisitions and Grants

About this file

This document is a technical specifications file for Quantitative PCR (QPCR) Systems, part of a sources sought announcement by the US Geological Survey (USGS). The technical specifications outline detailed requirements for three QPCR systems, including sample capacity (two 96-well and one 384-well machines), reaction volume ranges (10-100 mL for 96-well, 5-20 mL for 384-well), and precise technical parameters such as temperature uniformity (0.4°C), temperature accuracy (0.25°C), and compatibility with multiple fluorescent dyes.

The sources sought announcement (Solicitation Number: 140G0325Q0091) is seeking market research to identify qualified businesses capable of providing these QPCR systems for highly sensitive early detection of invasive species. Responses must be submitted electronically to yangzhideng@usgs.gov by 04/18/2025 at 1500 PST, using NAICS code 334516 (Analytical Laboratory Instrument). The procurement is for the Department of the Interior's US Geological Survey Office of Acquisitions and Grants, with a Product Service Code of 6640 for Laboratory Equipment and Supplies. This is a market research effort and not a formal solicitation, with no commitment to contract award.

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Text version

QPCR Systems technical specifications:

Sample capacity (wells): two 96 well machines, one 384 well machine Reaction volume: 96-well block: 10–100 μL; 384-well block: 5–20 μL Excitation source: Bright white LED Optical detection: At least 5 filters Excitation/detection range: at least 450–650 nm/500–700 nm Multiplexing: 96-well: up to 6 targets, 384-well: up to 5 targets Maximum block ramp rate: 6.0°C/sec Average sample ramp rate: 3.66°C/sec Temperature uniformity: 0.4°C Temperature accuracy: 0.25°C Compatible dyes: FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/ Texas Red, Mustang Purple™, Cy5/LIZ™, Cy5.5

File details come from the government source that posted it. Updated .