Purchase Description_final_.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The purpose of this requirement is to replace an iXon3 camera purchased eight (8) years ago which is no longer functioning optimally and is at the end of its term of use.

The iXON Life EMCCD camera will be a considerable update in functionality and enable the laboratory to produce better results. This requirement will also include Smart Shutter components, to improve the signal quality in the experiments and for automated opening and closing of a light path on a microscope.

2. Background Information and Objective:

The National Institute of Neurological Disorders and Stroke (NINDS), Synaptic Transmission Section (STS) laboratory is working on endocytosis. Synaptic vesicle endocytosis retrieves fused vesicles from the plasma membrane to sustain synaptic transmission, which is essential for cell functioning. The STS laboratory is conducting research on endocytosis by live imaging, electron microscopy, western blot, and molecular biology, and involving several genes.

3. Generic Name of Product:

iXon Life 897 Life Electron Multiplying CCD (EMCCD) camera and SmartShutter components

4. Purchase Description:

* iXon 897 Life EMCCD camera

-NIS-Elements Camera Integration Set Up

* Shutter Components -35MM Smartshutter with Stand Alone Housing (use 35mm EC or TL adapters) -Smartshutter Controller with USB Cable -Excitation Adapter: Smartshutter. 32mm filter wheel

--NIS-Elements: Hardware Module: Wavelength control - device control for Sutter, Prior and Unibliz illumination devices including shutters and filter wheels.

5. Salient characteristics:

iXon 897 Life EMCCD camera Technical Specifications

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

Sensor BV: Back Illuminated, standard AR coated Active pixels 512 x 512 Pixel size 16 x 16 μm Image area 8.2 x 8.2 mm with 100% fill factor Pixel Readout Rate 17 MHz - 10 MHz Minimum temperature, air cooled, ambient 20°C -70ºC '-70ºC Chiller liquid cooling, coolant @ 10°C, >0.75l/min -80ºC '-80ºC Thermostatic Precision ± 0.01°C

Triggering Internal, External, External Start, External Exposure, Software Trigger

System window type UV-grade fused silica, Broadband Visible- Near Infrared, 0.5 degree wedge

Blemish specification Grade 1 sensor from supplier Digitization 16-bit (at all speeds) PC Interface USB 2.0 Lens Mount C-mount Active area pixel well depth (e) 80,000 Frame rates (fps) 26 (full frame) - 9,610 Read noise (e-) < 1 with EM gain QE max > 95% (EX2 available)

6. Quantity: 1

7. Delivery Date: 60 days of the receipt of the purchase order

8. Period of Performance:

9. Warranty: Two (2) years for the shutter, one (1) year for the camera electronics and seven (7) years for vacuum integrity on the sensor.

10. Option(s):

N/A