Purchase Description_final.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The National Institute of Mental Health (NIMH), Unit on the Neurobiology of Affective Memory (UNAM) and the NIAAA Laboratory on the Neurobiology of Compulsive Behaviors (LNCB) require the acquisition of more ready to use (turnkey) fiber photometry (FP) systems to complement their current Tucker Davis Technologies (TDT) systems. Projects being pursued by the UNAM and NIAAA requires the acquisition of more ready to use (turnkey) fiber photometry (FP) systems to complement their current FP systems. The UNAM and LNCB labs have utilized preexisting Tucker Davis Technolgies (TDT) fiber photometry systems within the NIMH and the NIAAA. The needed systems meet all the criteria for the laboratories and are solely obtainable from TDT. Large ongoing data subsets, and experiments have been obtained and are ongoing via the existing TDT FP system. Due to the two 2 laboratories growing reliance on fiber photometry in their standard experimental paradigms they need to acquire two (2) more TDT FP systems.

2. Background Information and Objective: Current approaches to investigations of brain circuits and systems require detailed analyses of neuronal circuit dynamics at a cellular or greater resolution across multiple brain regions. Fiber photometry is a neural imaging technique used to monitor neural activity of specifically targeted cell populations. Fiber photometry uses excitation light from implanted fiber optics to record fluorescent activity of genetically encoded calcium indicators in neuronal populations that fluoresce in the presence of calcium and or chlorine. This imagining can be uniquely used while behavioral or electrophysiology experiments are being conducted giving a better understanding of the underpinning of brain function and its role in animal behavior in free roaming animals. The systems needed by UNAM and LNCB are solely obtainable via Tucker Davis Technologies. Due to the UNAM and LCNB growing integration of fiber photometry into their standard experimental paradigms their current systems are overtaxed and leading to shortfalls in productivity. The systems must match the existing Tucker Davis Technologies fiber photometry systems due to data sets already obtained and for scientific continuity and reproducibility of existing and future experiments.

3. Generic Name of Product: Fiber Photometry Workstation

4. Purchase Description:

(1) Fiber Photometry Workstation

(2) Lux Fiber Photometry Workstations plus accessories

(3) Tucker Davis Technologies customized base unit Lux Fiber Photometry Workstation:

Model and Part numbers:

-6-Channel LED Driver

-4 Photosensor Inputs with accessories (3) Product # RZ10x quantity 1 (complete systems see quote) -Lx405, quantity 2, -Lx465, quantity 2 -Lx590, quantity 2 -LxPS1, quantity 2 -LxPM1, quantity 1 -LXFX-KIT-3C, quantity 2 -LXFC-600-50-2M, quantity 1 -WS-4, quantity 1 -Monitor, quantity 1 -iCon2, quantity 1, -iH10, quantity 2

5. Salient characteristics

1. Must be a ready to use (turnkey) system for lock-in amplifier method of fiber photometry.

2. Must allow for high-fidelity signal detection in noisy environments with parasitic room lighting.

3. Must allow for easy integration of stimulation, third-party behavioral box communication, and closed-loop paradigms with no delay in processing.

4. Suitable analog inputs and outputs to control LED or LED drivers, and receive the fiber photometry feedback.

5. Must allow for control of multiple LEDs and photosensors at once.

6. Shall allow integration of other behavioral devices with the FP system with a high level of temporal resolution.

7. Must allow the ability to integrate simple video recording (with integrated playback of video alongside the recorded physiological data).

8. All data should be in a single format and time stamped with a precision of 1 mSec or less. This includes behavioral signals and video inputs.

9. System must be built around multiple real-time digital signal processors-not analog.

10. Processor must drive up to 6 LEDs and 4 photosensors for multi-color, multi-site recordings.

11. The DC offsets can be automated to prevent clipping. For long, multi-hour experiments, timing cycles can be designed to turn fiber photometry recordings on and off.

12. Must have 24 channels of programmable digital I/O. The software must provide for easy integration of external behavioral devices with the system, with a jitter of less than 20 microseconds.

13. The software must include ability to integrate with up to two USB cameras for integrated video recording. The software must also feature concurrent, time-aligned playback of video and all other data.

14. Must easily integrate behavioral signals with the fiber photometry system without the use of open source software or need to purchase second party systems to integrate with behavioral boxes.

15. Must be a flexible system for the lock-in amplifier of FP allowing for recording of two or more signals from one photosensor.

16. The systems drivers and sensors must be compatible with all commonly used optogenetics equipment, including those made by Doric, Thor Labs, and Prizmatix.

17. The system must feature up to six integrated LEDs and four photosensors for 3 LED fiber photometry in 2 sites or animals.

18. The system must be built around real-time digital signal processors, and have extensive processing capabilities and programmability, making it possible to perform custom processing as well as dynamic control.

19. The system must allow up to two common source USB cameras to be connected for integrated video recording. The software must allow for easy playback of the time-locked video and all other data.

20. The software must include a library that allows any programming language compatible with Windows ActiveX controls or HTTP to control the system or analyze data (which includes Matlab).

21. The system shall allow for detecting several signals simultaneously off the same fiber optic cable (405 isosbestic, 465 GCaMP, 560 TDtomato.

22. System must allow interchangeable LED.

23. Software must allow for auto-detect of installed LED wavelengths

24. The behavioral control module must allow for controlling behavioral operant chambers including modules for controlling high voltage hardware, IR sensors, auditory stimulation, lickometers, digital triggers etc. the behavioral control module should directly connect to the processor and the software allowing for control of fiber photometry and behavioral paradigms from the same software, on the same computer.

6. Quantity: Two (2) complete customizable systems

7. Delivery Date: 30 days after contract award

8. Period of Performance:

9. Option(s): n/a