Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: 10X Genomics Chromium Consumables

2. Background Information and Objective:

Single-cell multiomic assays will be used to map the epigenetic and transcriptomic signatures in peripheral blood mononuclear cells from participants in the Baltimore Longitudinal Study on Aging. This work seeks to identify peripheral signatures that could predict the onset of cognitive impairment and Alzheimer’s disease (AD). This project is a newly funded AD concept proposal sponsored by the NIA.

4. Generic Name of Product: single-cell multiomic assays

5. Purchase Description:

1000283 Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 16 rxns Contains the components from the following Single Cell Multiome ATAC+Gene Expression Reagent Kits: ATAC Kit A (1000280), Reagent Kit A (1000282), and Library Construction Kit (1000190), 16 rxns - Quantity 2

1000234 Chromium Next GEM Chip J Single Cell Kit, 48 rxns For Single Cell Multiome ATAC + Gene Expression. Contains the Next GEM chips, gaskets and oil for sample partitioning, and the recovery agent for coalescing GEMs post barcoding, 48 rxns - Quantity 1

1000215 Dual Index Kit TT Set A 96 rxns Contains 96 unique dual i7 and i5 sample indices to enable indexing of Single Cell 3’ v3.1(Dual Index), Single Cell 5’ v2 Gene Expression and TCR/BCR, Visium Spatial Gene Expression, Single Cell Multiome ATAC + GEX (GEX library) and Fixed RNA FB Libraries = Quantity 1

1000212 Single Index Kit N Set A, 96 rxn Contains 96 unique i7 single index reagents for multiplexing samples on illumina flow cells. Use with Chromium Next GEM Single Cell ATAC and 5' Feature Barcode and Single Cell Multiome ATAC + GEX (ATAC library) – Quantity 1

6. Delivery Date: 30 days after receipt of award