Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Statement of Need and Purpose: This acquisition is necessary to provide a flow cytometer to the Center for Alzheimer’s and Related Dementia for neurodegenerative disease research.

Background Information and Objective: imaging technology improves to capture The National Institute on Aging (NIA) requires a cytometer that will provide intra-laboratory high content flow cytometry for the laboratory. The required equipment must be compatible with current research protocols within the NIA and compatible with data and software on the current Flow Cytometry research facility which provides basic research support to members as well as to investigators throughout NIA.

The research projects supported are complex and varied. As such, the laboratory is required to maintain equipment that provides for a wide range of applications and that includes the ability to modify and adapt capabilities as new applications are developed.

In general, this requirement is satisfied by utilizing instrumentation with a wide range of laser excitation wavelengths and power outputs combined with the ability to custom configure fluorescence detector capabilities.

The laboratory requires a Flow Cytometer Analyzer for research. The demand for this instrument is expected to increase further with the addition of more studies. The need for high power lasers and fluorochrome detection capability is required. The required instrument must provide 4 separate lasers and 16 color detection capabilities.

Generic Name of Product: Laser Flow Cytometer

Purchase Description:

Salient characteristics All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Vendor must be able to provide these specifications:

- Must have the capability of being utilized by multiple users for a variety of applications in immunobiology and molecular immunology.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

- Must have the ability to customize and configure fluorescence detector capabilities, including user access to, and ability to change, fluorescence band pass and long pass filters which are interchangeable between the existing and proposed instrument.

- Must be able to collect pulse area, width and height on all parameters simultaneously

- Must be able to export acquisition data in flow cytometry standard (FCS) 3.0 and 3.1 file format

- Must have sixteen (16) color simultaneous detection plus FSC and SSC from four (4) lasers

- Must have the ability to incorporate five (5) spatially separated lasers with automatic time delay calculation using instrument set-up software.

- Must have variable detector configurations using reflected light decagon PMT arrays.

- Must have the capability to detect less than 15 MESF particles on PE channel

- Must have the capability of resolving .05um beads from noise on Side Scatter Channel.

- Optical design must be able to deliver greater than 80% of stated laser power at the flow cell.

- Must be able to have a Fluorescence Linearity Doublet Singlet Ratio of 1.95-2.05 for CEN Stained with PI.

- Must incorporate all digital electronics to provide data acquisition rates up to 40,000 events per second with beads and 10,000 events per second with cells.

- Must incorporate manual (not motor driven) sample change capability, and provide for sample carryover of less than 0.1% within 5 seconds.

- Must have integrated laser power control software that provides for turning power on/off and adjustable power level control for each of the lasers independently.

- Must be able to acquire data with real time statistics.

- Must have four spatially separated lasers with the following wavelength (nm) and power output maximum(milliwatts):

a. 405 nm, 100 milliwatts

b. 488 nm, 100 milliwatts

c. 640 nm, 100 milliwatts

d. 561nm, 100 milliwatts

- Must have fully integrated automatic sample injection from microliter plates (96 or 384 well) with operation controlled by the cytometer software. Maximum carryover .05ul between samples

Delivery Date: Delivery date of 2 months after receipt of completed purchase order