Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: This acquisition is necessary for automation of the culture techniques used in the iPSC Neurodegenerative Diseases Initiative (INDI) Project within the new Center for Alzheimer’s Disease and Related Dementias.

2. Background Information and Objective: imaging technology improves to capture We are working to create a national resource of isogenic sets of Alzheimer’s Disease and Related Dementias mutated iPSC lines, complimented by foundational phenotypic datasets from differentiated disease-relevant cells derived from these lines. This resource will substantially accelerate the pace of mechanistic discovery regarding how ADRD mutations cause disease. An automated liquid dispensing device is needed to create a high through put workflow for processing tissue culture cells to enable genetically engineered iPSCs.

3. Generic Name of Product: Microplate Liquid Handler, Multi-Mode Dispenser, Plate Stacker, and Multi-mode Reader

4. Purchase Description:

Description: Cat # Quantity MultiFlo FX with 2 module arms, MFXP2-SN 1.00 Secondary Peristaltic Pump Dispenser Module 7210010 1.00 Dual Syringe Dispenser Module 7210008 1.00 Strip Washer Module for MultiFlo FX Multi-Mode Dispenser 1260023 1.00 Periwash Module Complete 1260041 1.00 Cassette Periwash ASBY 1260035 2.00 Dual Syringe Dispenser Module - Autoclavable 7210008 1.00 Cassette Periwash ASBY 1260035 6.00 Periwash Manifold Set ASBY 1260036 6.00 Peristaltic Pump Cassette 5-2500 uL 7170011 1.00

SVCE MANF 16 TUBE 7 DEG ASBY 7180543S 2.00

SVCE MANF 6 WELL 7 DEG ASBY 1260537S 1.00

SVCE MANF 12 WELL 7 DEG ASBY 1260536S 1.00

SVCE MANF 24 WELL 7 DEG ASBY 1260535S 1.00

Access 96/384 Well WSHR MANF 1260013 1.00

Access 24 Well Washer Manifolds 1260011 1.00 Access 12 Well Washer Manifolds 1260010 1.00 Access 6 Well Washer Manifolds 1260009 1.00 Cassette Periwash ASBY 1260035 1.00 Periwash Manifold Set ASBY 1260036 1.00

ACCESSORY WEIGHT SET 1230008 1.00

BioStack4 BSTCK4-SN 1.00

NEO2 W/DBL TP FLTR MON BOT FLTR TRF N2MBT-SN 1.00

ACCESSORIES STACK SET 30 PLATE 7310008 1.00

Cube 3 Single PMT TOP ASBY 1035003 1.00 Cube 101 485/516 LUM ASBY 1035101 1.00 Micro-Volume Plate: TAKE3 1.00 Packaging and Handling NA 1.00

Salient characteristics All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Vendor must be able to provide these specifications:

Microplate Liquid Handler must be able to provide these specifications:

Must have Agilent VWorks software, integrated with BioTek Gen5 and LHC2

Must be able to integrate and fully compatible with Agilent Synergy Neo2 Multi-Mode Reader, Cytation 5 Imaging Reader, Epoch 2 Absorbance Reader EL406 Washer Dispenser, MultiFlo FX Multi-Mode Dispenser

MultiFlo FX Multi-Mode Dispenser must be able to provide these specifications:

Must have ability to dispense up to four independent reagents without cross contamination.

Must be modular and upgradeable from one to four reagent dispensers.

Must have touchscreen user interface.

Must be compatible with 6-, 12-, 24-, 48-, 96-, 384- and 1536-well microplates.

Must have available single channel random access dispensing for 6-, 12-, 24-, 48-, 96-, and 384-well microplates.

Must be able to dispense individual volumes into individual wells, using an imported custom plate map.

Must have available plate washing capability for 6-, 12-, 24-, 48-, 96-, and 384-well microplates.

Must have ability to provide gentle media exchange without loss of 3D cell structures.

Must have broad dispense volume range from 500 nL to 30,000 μL/well.

Must have 1 μL, 5 μL and 10 μL cassettes available for optimal peristaltic pump performance.

Must have angled tubes for gentle dispensing with cell-based assays.

Must be compatible with deep well microplates, PCR trays and microtubes.

Must have both peristaltic pump fluid delivery and dual syringe drive fluid delivery options.

Must be automation compatible.

Must have an available optional microplate stacker.

Must be able to dispense 10 μL into 96 wells in 3 seconds with the peristaltic pump.

Must be able to dispense 5 μL into 384 wells in 6 seconds with the peristaltic pump.

Must have user programmable shaking and soaking.

Must have optional autoclavable sterilization compatibility.

Must have USB port for protocol transfer and storage.

Must have extensive onboard software for protocol creation and execution.

Must have optional pc-based software for protocol programming and execution.

Multi-Mode Reader must be able to provide these specifications:

Must have hybrid optical system combining an independent monochromator fluorescence optical system with an independent filter-based fluorescence optical system in the same instrument.

Must have multiple parallel detectors for ultra-fast simultaneous measurements.

Must have quadruple scientific grating optical design (two gratings per monochromator) for better spectral scanning performance.

Must have continuously variable bandwidth monochromators for fluorescence measurements in 1 nm increments.

Must be capable of performing fluorescence and absorbance spectral scans.

Must be upgradable to laser-based excitation source for Alpha assays.

Must have 337 nm laser for TRF assays.

Must have dedicated filter-based bottom optics with focus adjustment for live-cell assays.

Must have fiber-free filter-based optics for increased optical efficiency.

Must have maximum read speed of 6 seconds for 96-well plates and 11 seconds for 384-well plates, including for dual measurement fluorescence polarization assays.

Must have stacker option, with plate exchange speeds of 6 seconds.

Must have image-based 1D and 2D barcode scanner option.

Must be able to automatically dispense reagents in all detection modes.

Must be compatible with 2 μL low volume microplate accessory with 16 or 48 sample microspots for direct nucleic acid quantification as well as fluorescence and luminescence measurements.

Must be able to control temperature to 70 °C with a 4-zone temperature control system, with the ability to set a temperature gradient to prevent condensation on plate lids.

Must have top/bottom automatic focus adjustment to automatically accommodate for various microplate heights and fluid levels. Must have automatic individual well-by-well absorbance path length correction to automatically calculate 1 cm path length corrected results and compensate for pipetting errors in microplates.

Plate Stacker must be able to provide these specifications:

Must be able to accommodate these types of microplates: ANSI/SBS standard and low profile 96, 384, and 1536 wells, and 24 and 48 well plates with plate height 23.

Must provide capability for de-lidding 96, 384, and 1536 plates, as well as de-lidding Nunc plates 6, 12, 24, and 48 well plates

Must allow for the following capacity: 10 and 30 plate stacks to be removable and interchangeable. 96/384 well plates up to 30 plates with lids, 1536 well plates up to 75 plates

Must provide for barcode scanner in landscape or portrait orientation, code 39, UPC/EAN, Code 128 compatible 1D and 2D barcodes

Must be able to provide a processing speed of <20 seconds with de-lidding, and <12 seconds without lids

Must be able to provide direct control of washer and dispenser with keypad interface and can directly control Biostack

Must provide LHC2 software for the liquid handler and Gen5 for reader.

5. Quantity: 1 complete unit with one year manufacturer warranty.

6. Delivery Date: Delivery date of 3 months after receipt of completed purchase order