Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: This request is to procure a single cell sequencer.

This instrument is an important component of our efforts to systematically analyze induced pluripotent stem cells (IPSC) associated with neurodegenerative disorder research for gene expression analysis in the Center for Alzheimer’s Disease and Related Dementias.

2. Background Information and Objective: The Center for Alzheimer’s Disease and Related Dementias is planning to perform a large project using 200 iPSc lines differentiated into forebrain neurons, astrocytes and microglia. It will be aimed to perform single cell RNA sequencing for these samples to learn cell type-specific gene expression and the identification of genetic associations with gene expression levels in iPSC derived cells.

3. Generic Name of Product: Single Cell Sequencer

4. Purchase Description - Salient characteristics All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Vendor must be able to provide these specifications:

Instrument shall deliver paired full-length T-cell receptor alpha and beta chain sequences at the single cell level using universal and unbiased amplification strategies.

Instrument shall incorporate of a unique molecular identifier (UMI) in the template switch oligo to enable transcript quantification and further reduce systematic bias by controlling for non-biological SNVs or other variants introduced by PCR and sequencing error.

Instrument shall enable the detection of germline and somatic variation across full-length antigen receptor V(D)J gene sequences.

Instrument shall identify both productive and non-productive recombination events as well as adaptive immune cells with more than one productive T-cell receptor alpha or beta chain.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

Instrument shall map transcription start sites.

5. Quantity: One (1)

6. Delivery Date: Delivery date of 2 months after receipt of completed purchase order

7. Period of Performance: Manufacturer warranty for one calendar year beginning date of receipt.

8. Option(s): Not applicable