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PURCHASE DESCRIPTION
1. Statement of Need and Purpose: NIA utilizes single cell omics/cell editing techniques (e.i. CRISPR), and requires equipment to efficiently separate out single cells, and placed it into into 96- and 384-well format.
2. Background Information and Objective: Single-cell omics, Low-volume assays (WGA, PCR, NGS setups) and cell line production are fast developing scientific areas. Single cell omics required appropriate instruments for this work and suggested equipmets will serve as approriate tool.Currently NIA has a QPCR machine where we can analyze 384-well plates. The process of aliquoting reagents by hands to 384-wells currently take about 1.5 hour with reduced reproducibility. The objective is obtaining a Robotic cell sorting/ washing station with the ability to make 1 plate in 25 seconds with very low error. Combination of these two instruments will give the National Institute on Aging (NIA) a huge increase in productivity, when NIA can separate and analyze 384 single cells within several minutes.
3. Generic Name of Product: Robotic single-cell printer with washing station, for handling liquid cell suspension for WGA,PCR, NGS preparation..
4. Purchase Description: F.SIGHT OMICS (single cell priner); I.DOT DW (Non-contact, low volume dispensing system), C.WASH (centrifugal plate washer). Company Dispendix (CELLINK LLC)
3. Salient characteristics: NIA requires a robotoc single-cell printer with washing station with the following characteristics:
F.SIGHT OMICS (Cell Printer):
| Dimensions (W x D x H) |
| 600x400x240 (mm) |
| Power sockets required |
| 2x |
| Input Voltage [VAC] |
| 100-240 |
| Input voltage frequency [Hz] |
| 50/60 |
| Max. input current [A] |
| 1.4 |
| Max. power consumption [W] |
| 135 |
| Input fuses |
| 2x 3.15 A quick react |
| Internal voltage levels [VDC] |
| 5, 12, 24 |
| LED source 1 (DQC) |
| white 1 W |
| LED source 2 (Detection) |
| red 3.0 W |
| Embedded Laser source 1 |
| 473 ±1 nm 100 mW ±5 % |
| Embedded Laser source 2 |
| 488 ±1 nm 100 mW ±5 % |
| Embedded Laser source 3 |
| 561 ±1 nm 100 mW ±5 % |
| Laser protection class DIN EN 60825-1: 2015 |
| Class 1 |
FDA 21 CFR 1040.10 & 1040.1
| microfluidic outlet (nozzle) |
| 40 x 40 ìm |
| Included warranty |
| 12 months |
I.DOT DW (Non-contact, low volume dispensing system):
| Feature Specification |
| 471 mm x 575 mm x 378 mm |
| Dimensions (L x W x H) |
| AC 100-120 V, 50/60 Hz, 10A or AC 200-240 V, |
| Power supply |
| AC 100-120 V, 50/60 Hz, 10A or AC 200-240 V, |
50/60 Hz, 5A
| Compressed air supply |
| Filtered, oil-free, dry air, 6–10 Bar (8–145 PSI) |
| Destination |
| All SBS-format microplates not exceeding 127 mm |
x 85 mm x 54 mm (L x W x H)
| Dispensing volume per well |
| Standard plate: 8 nL to 80,000 nL (80 μL) |
Deep reservoir plate: 8 nL to 500,000 nL (500 μL)
| Dispensed volume CV |
| <5% (for >100 nL) |
<8% (for <100 nL)
| Liquid viscosity range |
| 1 to 100 cP (mPa-S) |
| Nozzle diameter |
| 60 um to 200 um |
| Source fluid temperature range |
| 4° C to 37° C |
(requires the Cooled Source Tray for low temperature)
| Temperature accuracy |
| ±0.5° C |
| Target plate temperature range |
| Room temperature |
C.WASH: C.WASH centrifugal plate washer. Includes instrument with pump for waste removal, touch pad, user-friendly software, free software updates for 12 months, 4 liquid input channels, 2 standard carriers for 96/384-well plates, power cables, and 2 500 ml glass bottles for media, angled 8-pin and 16-pin dispenser for 96/384-well plates, 1x 500 ml C.WASH cleaning reagent.
Quantity: F.SIGHT OMICS (single cell priner)- 1; I.DOT DW (Non-contact, low volume dispensing system) - 1, C.WASH (centrifugal plate washer) – 1.
4. Delivery Date: 30-60 days after receipt of order Version 11-30-2016-1
FAR Part 11—Describing Agency Needs