Purchase Description Chromium X.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The Chromium X will be used for single-cell genomics assays proposed in NIA-funded grants.

2. Background Information and Objective: Single-cell genomics has revolutionized how we think about biology, and cellular heterogeneity. 10X Genomics has contributed to this revolution by technological innovations that simplify single-cell applications. The Computational Biology and Genomics Core (CBGC) has the first generation of 10X Chromium Controllers that were acquired in 2018-2019. Given that the number of single-cell projects at NIA-IRP has grown over the years, it is now necessary to upgrade the 10X Chromium Controllers to the newest and higher throughput Chromium X with special discount pricing. Chromium X offers high flexibility allowing not only to run high-throughput single-cell assays at a fraction of the cost but also the completion of ongoing projects with previously purchased low-throughput kits. Many IRP labs including LGG (Gorospe, Sen, Wang), LMBI (Sen, Weng), TGB (Ferrucci, de Cabo), LCS (Lakatta), LCI (Egan) and others will be benefitted if this instrument is added to the CBGC facility.

CBGC has other instruments and expertise required for 10X Chromium X to work seamlessly like Illumina Novaseq 6000 sequencer as well as trained staff for running both the wet-lab and dry-lab protocols. Adding this instrument to the core will have a cost-saving benefit for the institute as it will reduce the overall cost of running 10X single-cell assays to investigate aging and age-related diseases.

3. Generic Name of Product: Chromium X

4. Purchase Description:

Trade name: 10X Genomics Brand name: 10X Genomics Manufacturer’s name: 10X Genomics Model and type: Chromium X & Accessory Kit, Chromium Next GEM Single Cell HT Training Kit Catalog numbers: 1000331, 1000386

5. Salient characteristics:

The unique platform characteristics are:

● Generate single-cell transcript counts from up to 80,000 cells per run

● Produce >90,000 individual nanoliter-scale partitions leveraging ~3.6 million unique barcodes

● Capture cells at high efficiency up to 65%

● Low doublet rate of 0.9% per 1,000 cells.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

● Achieve deep profiling of complex cell populations with high-throughput digital gene expression of individual cells

● Access the whole transcriptome with an unbiased approach appropriate for discovery work, or focus on a subset of relevant target genes by performing target enrichment of the same library sample prior to sequencing

● Measure additional phenotypes with Feature Barcoding technology

● Provide turn-key analysis pipelines and visualization software for novice and expert users alike

● Include a microfluidics chip that is compatible with a wide range of eukaryotic cell sizes and types (e.g. adherent cell cultures, suspension cell cultures, tissues, blood, etc.)

● Rapid time to lysis provides an accurate view of the transcriptome with minimal impact on expression

6. Quantity:

Chromium X & Accessory Kit - 1 Chromium Next GEM Single Cell HT Training Kit - 1

7. Delivery Date: Within 60 days