PURCHASE DESCRIPTION Chromium Controller.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The Computational Biology & Genomics Core

(CBGC) is in need of a new 10X Genomics Chromium Controller for higher throughput single-cell transcriptomic and epigenomic data.

2. Background Information and Objective: Single-cell sequencing has revolutionized how we think about biology, and cellular heterogeneity. 10X Genomics has contributed to this revolution by technological innovations that simplify single-cell applications. The CBGC has the first generation of 10X Genomics Chromium Controllers that were acquired in 2018. Given that the number of single-cell projects at the National Institute on Aging-Intramural Research Program (NIA-IRP) has grown over the years, it is now necessary to upgrade the 10X Genomics Chromium Controllers to the newest and higher throughput Chromium X. Chromium X offers high flexibility allowing not only to run high-throughput single-cell assays, but also the completion of ongoing projects with previously purchased low-throughput kits. Many IRP labs including Laboratory of Genetics & Genomics (LGG), Laboratory of Molecular Biology & Immunology (LMBI), Translational Gerontology Branch (TGB), Laboratory of Cardiovascular Science (LCS), Laboratory of Clinical Investigation (LCI) and others will benefit if this instrument is added to the CBGC facility. CBGC has other instruments and expertise required for the 10X Genomics Chromium X to work seamlessly such as the Illumina Novaseq 6000 sequencer as well as trained staff for running both the wet-lab protocols and data analysis pipelines. Adding this instrument to the core will have cost-saving benefit for the institute as it will reduce the overall cost of running 10X Genomics single cell assays to investigate aging and age-related diseases. In fact, the cost saving per cell will pay for the cost of this instrument upgrade within a short time.

3. Generic Name of Product: Chromium Controller

4. Purchase Description:

Chromium X & Accessory Kit, 12 Month Warranty – Product Code 1000331

Chromium Next GEM Single Cell HT Training Kit, 32 rxns – Product Code 1000386

5. Salient characteristics

Shall be flexible, reliable, scalable, compact, benchtop system for single-cell sample preparation for different single-cell assays.

Shall be compatible with single-cell gene expression, ATAC-seq, immune profiling and single-cell multiomics.

Shall be capable of preparing hundreds of thousands of cells.

6. Quantity: 1

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

7. Delivery Date: 30 Days ARO

8. Period of Performance: N/A

9. Option(s): N/A