Purchase Description-75N95023Q00671.pdf
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- Attached to
- Chromium Next GEM Consumables Federal contract opportunity
- Solicitation number
- 75N95023Q00671
About this file
This purchase description outlines requirements for consumables for a Chromium Next Generation Experimentation (GEM) system. The National Institute on Aging Laboratory Genetics and Genomics RNA Regulation Section requires one Chromium Next GEM Single Cell 3' Kit v3.1 for 16 reactions, four kits for 4 reactions each, and three Chromium Next GEM Chip G Single Cell Kits for 48 reactions each. The consumables must be compatible with the 10X Genomics Chromium instrument and support high gene sensitivity, low multiplet rates, and robust workflows with simple chip loading and compatibility with whole cells and nuclei. Delivery is required within 30 days after receipt of order under solicitation number 75N95023Q00671 issued by the Department of Health and Human Services National Institutes of Health National Institute on Drug Abuse for Chromium Next GEM Consumables.
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| File | Type | Posted |
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| FAR 52.212-5 Jun 2023.pdf | ||
| FAR 52.204-24 Nov 2021.pdf | ||
| Invoicing Instructions With IPP.pdf | ||
| FAR 52.212-4 Addendum.pdf | ||
| FAR 52.204-26 Oct 2020.pdf |
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Version 11-30-2016-1 FAR Part 11—Describing Agency Needs
PURCHASE DESCRIPTION
1. Statement of Need and Purpose: Chromium Next GEM Consumables.
2. Background Information and Objective:
The National Institute on Aging (NIA) Laboratory Genetics and Genomics (LGG), RNA Regulation Section (RNARS) uses the Chromium system enables the lab to capture between 10,000 to 80,000 single cells, mark them with a bar-code and a second unique molecular code that can be tagged to genomic DNA, or RNA. Following downstream procedures to isolate DNA or RNA and sequencing, these unique codes are read, deconvoluted and stratified to learn the nature of transcripts or status of DNA within each captured single cell. This procedure is highly valuable to distinguish the cellular complexity of a tissue based on the nature of transcription profile, identify DNA status in individual cells in terms of its DNA accessibility for transcription, nature of insertions and deletions.
There is a wide interest in the field to understand the composition of solid and hematopoietic tissues and define the nature of resident stem cells and their fate during aging. The method helps to distinguish cell types with different functions, which can then be marked with specific antibodies and followed during development, differentiation, and aging. This method will allow us to approach these goals with high efficiency. It is important to note that this system is much more “automatized” than other available methods, so that it is correspondingly more likely to get dependable data from precious samples.
3. Generic Name of Product: Chromium Consumable Next GEM
4. Purchase Description:
Chromium Next GEM Single Cell 3' Kit v3.1, 16 rxns (Quantity: 1) Chromium Next GEM Single Cell 3' Kit v3.1, 4 rxns (Quantity: 4) Chromium Next GEM Chip G Single Cell Kit, 48 rxns (Quantity: 3)
5. Salient characteristics:
• High gene sensitivity, low multiplet rates
• Compatible with Feature Barcoding technology for Cell Surface Protein profiling or
CRISPR Screening
• Robust workflow
• Simple chip loading
• Compatible with whole cells and nuclei
• Industry leading cell capture rates (~65%)
• Broad range of sample throughput (1-8 samples per chip)
Version 11-30-2016-1 FAR Part 11—Describing Agency Needs
• Broad range of cell throughput per sample (500 - 10,000 cells)
• Cell size flexibility - no lower limits
• must be compatible with the 10X Genomics Chromium instrument
6. Delivery Date: 30 days ARO
File details come from the government source that posted it. Updated .