Notice of Intent.pdf

This notice announces the intent by the U.S. Army Medical Research Acquisition Activity (USAMRAA) in support of the Armed Forces Medical Examiner System (AFMES) to procure 7500 HID Real-Time PCR instrument by Life Technologies Corporation without competition as prescribed in FAR 5.102(a)(6) and 5.203(a).

The 7500 HID Real-Time PCR instrument shall meet the following minimum characteristics with regards to the instrument:

• Optics: Must contain a CCD Camera with 5 excitation filters, 5emissions filters and a Tungsten-halogen lamp

• Shall be compatible with the following calibration dyes: SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™

• Shall contain a thermal Peltier-based thermal cycling system with a heat block that must have a temperature range of 4-100°C with a peak block rate of 2.5°C/sec and a sample rate of +1.1°C/sec.

• Shall be configured in a 96 well plate format and be forensically validated for use with 96 well plates with a reaction volume of 20-100µl.

• Shall be compatible with GeneMapper™ IDX v1.0 or higher, 7500 System Software, HID Real-Time PCR Analysis Software v1.1 or higher.

• Shall use ROX as a passive reference dye

• Shall have a sensitivity to 1 copy of Rnase P gene (Human Genomic DNA) and have a precision of 99.7% confidence between 5000 and 10000 copies.

• Shall be able to run 4 to 5 96 well plates in an 8 hr day.

System Components and Operating Specifications:

• Shall include a computer workstation with flat screen monitor compatible with Windows 10, 64 bit IOT Enterprise operating system that is TPM 2.0 compliant.

• Integrated software for instrument control, data collection, quality control, and auto-analysis of sample files for quantification of samples.

• Tungsten Halogen lamp

• Voltage – 120V

• Dimensions: Width: 34 cm (13.39 in), Depth: 45 cm (17.72 in), Height: 49 cm (19.29 in)

• Peltier Based thermal cycling system configured 96 well plate format.

• Shall have a temperature uniformity of 0.50°C after 30sec and a temperature accuracy of

0.25°C between 35°C to 95°C after 3 min.

Computer Requirements shall be: A45279 - Optiplex XE3 500GB Tower Spec with Win10 IOT 2019- 64Bit, ROHS

Base Unit: OptiPlex XE3 Minitower Operating System: Windows 10 IOT Enterprise LTSC 2019, 64-bit, English, embedded Processor: Intel Core i7-8700 (6 Cores/12MB/12T/up to 4.6GHz/65W) Memory: 16GB (2x8GB) DDR4 2666MHz UDIMM Non-ECC Keyboard: US English (QWERTY) Dell KB216-B QuietKey USB Keyboard Black Video Card: AMD Radeon R5 430, 2GB, FH (DP/DP) Integrated VGA Video: Included, details (not used with add on video card) Hard Drive: 2 x 500GB 2.5 inch SATA 3.0Gb/s and 8MB Data Burst Cache Hard Drive Controller: RAID1, Combo Full Height Riser 1PCI/1PCI-E Mouse: Dell USB Laser 6-Button Mouse

Network Interface Card: 3 ports: Motherboard & Intel DP 10/100/1000 PCIe Gigabit Networking Card, Full Height

The North American Industry Classification System (NAICS) for this requirement is 334516 and Product Supply Code is 6515.

This Special Notice of Intent is not a request for competitive quotes. Information received in response to this notice will be considered solely for the purpose of determining whether a competitive procurement is in the best interest of the Government.

All questions and responses concerning this notice shall be emailed to Brian Miller at brian.d.miller1.civ@health.mil.

Interested concerns must identify their capability in writing to the above email address listed above no later than 1:00 PM ET, Local 4 May 2023.

DVD-RW Drive: 8X Half Height DVD+/-RW Drive Speakers: Internal Dell Business Audio Speaker Power Supply: OptiPlex XE3 MT 360W up to 90% efficient PSU Power Cord: Power Cord,125V,2M,C13,North America mailto:brian.d.miller1.civ@health.mil