H_Redacted JOFOC 2000347 Speed Biosystems Transfection Reagent.pdf

Justification for Other than Full and Open Competition at or Below the Simplified Acquisition Threshold

Project Title: __ Turbo293 Transfection Reagent for HEK293 Cells___

I . Identification of the agency and contracting activity

DHHS, NIH, NIAID,

II. Description of the project, and supplies or services required to meet the agency’s needs (including the estimated value) (This section to be completed by the requestor.)

A. Type of action. Check the appropriate box:

☐ New requirement ☒ Follow-on

• Name of the previous contractor(s): ___Speed Biosystems_

• Sequence number(s), if available:1875995, 1867132 1840226 ,1804124, 1933960

• Was the initial and any subsequent awards competed? ☒ Yes ☐ No

B. AMBIS Sequence number for this requirement:

C. Total estimated dollar value of the requirement (including options, if applicable): _ D. Period of performance, inclusive of options: from __30 days ARO E. Project Background

The Vaccine Research Center is developing vaccines for HIV-1 and other viruses. Production of various wild type and mutant viral proteins and anti-viral antibodies is critical in vaccine development. Making different mutations of viral proteins and expression antibodies against such proteins is critical for vaccine development. To make these proteins in transient transfection method, transfection reagent is critical to the success of protein and antibody productions.

F. Description of the supplies or services to be purchased: The SBS makes protein immunogens and antibodies for vaccine studies using transient transfection method. the quoted transfection reagent is needed in a regular basis to satisfy daily protein production requests

III. Identification of the statutory authority permitting other than full and open competition (SELECT ONE)

☒ 41 USC 1901(e)(2) and FAR Part13.106-1: Only one responsible source

IV. Demonstration that the proposed contractor(s) unique qualifications or the nature of the acquisition requires use of the authority cited. (This section is to be completed by the requestor.)

A. Describe why only the requested supply/service will meet the government’s needs

The goal of the VRC is to translate basic research into concrete vaccine products that can be rapidly deployed to protect public health. The SBS makes protein immunogens and antibodies for vaccine studies using transient transfection method. The quoted transfection reagent is needed in a regular basis to satisfy daily protein production requests.

B. Describe why only the proposed contractor can provide the requested supply/service

The Speed Biosystems reagent has been tested in frequent protein production batches to confirm consistency, and is already in use current ongoing experiments, changing variables may compromise results. Additionally, the propose transfection is specially made for the cell culture system we are using. Speed Biosystems is the sole source to purchase the reagent.