J_JOFOC_-_REdacted.docx

Sole Source Justification

Project Background

The Molecular Genetics Section (MGS) is investigating the molecular basis of cblX syndrome; a complex disease with biochemical and neurological phenotypes. The disease is caused by mutations in the cellular coactivator HCF-1. However, how these mutations translate into the disease phenotype is unknown. The MGS is using multiple approaches to investigate changes in the genome occupancy, HCF-1 protein complexes, and transcriptome that would contribute to the observed cblX phenotypes. To this end, the MGS has derived LIMITED primary fibroblast cells from humans and fibroblasts and brain tissue from mouse models of cblX. This request is for a second set of ChIP-Seq global cellular occupancy of the coactivator HCF-1 and RNAPII in these cells and tissues that is required for publication of these data sets.

Justification for Other than Full and Open Competition ☒ 41 USC 1901(e)(2) and FAR Part 13.106-1: Sole Source

The requirement has been determined a Sole Source requirement:

Active Motif provided the service for the first experimental data set, and to change vendors would create significant variability in the data based upon technical parameters used during the service. It is essential that the second (repeat) of the initial data set be done by the same provider using the same technical conditions. Changing the provider would introduce significant and unacceptable experimental variability in this ongoing project that would then require additional experimental sets and costs to the NIAID.