Purchase_Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The purpose of this acquisition is to procure a Patch Clamp

Rig and Bi-layer Recording Station

2. Background Information and Objective:

The National Institute of Neurological Disorders and Stroke (NINDS)-Molecular

Neurophysiology Section (MNS) studies the regulation and function of membrane proteins, focusing on the large-conductance voltage- and calcium-dependent potassium (BK) channels, Na+/K+ ATPase.. The first one is a Patch Clamp Rig (Port-A-Patch), which has the ability to be automated to allow for ionic current recordings, screenings and characterizations of the effect of ion chanel mutations. The Bi-layer recording system (the Orbit Mini) will be used to study the functionality of purified protein and the mutated purified proteins. This will allow to fuse the purified proteins in to the membrane and functionality studies can be done. Having both systems will allow easy transfer of data and membranes for complete anlaysis and must be compatible with each other.

3. Generic Name of Product:

Patch Clamp Rig and Bilayer recording station

4. Purchase Description: The contractor shall provide an elecrophysiology system and a patch clamp rig and bilayer recording station in accordance with the below salient characteristics:

5. Salient characteristics

Patch Clamp System:

a) Overall dimensions of the recording station shall not exceed 18 x 10 x 7 cm (7 x 4 x 3 in.)

b) The system shall support giga-seal recordings from one cell at a time. It shall offer access patch clamp data.

c) Suction control, USB-controlled (no in-house vacuum needed)

d) Software (Windows), with grphaical toolds for logging events

e) USB amplifier system

Bilayer Recording Station:

a) The complete bilayer recording platform shall consist of a main recording unit with a built-in four channel amplifier, and a computer-controlled, environmentally controled unit for active cooling and heating of the recording chamber. The system shall allow for use of disposable chips for manual painting of lipid bilayers and parallel recording from four bilayers.

b) System dimensions shall not exceed Maximum 1ft x 6” x 6”

c) System shall have an integrated four channel low-noise amplifier

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

d) System shall have the ability to target molecules that can be introduced directly by fusion of proteo-lipisomes

e) System shall have both voltage and ligand gated temperature sensitivie ion channels

f) USB-computer connection

6. Quantity: one of each system

7. Delivery Date: 8 weeks after receipt of the order

8. Training:

On-site training must take place within two (2) weeks of equipment installation. The equipment shall be delivered and installed between the hours of 8:00am and 5:00 pm, Bethesda, MD local prevailing time. The contractor shall provide an Original Equipment Manufacturer (OEM) certified service technician to perform setup, installation, and an on-site 1-day training session for 5 to 8 staff members.

9. Warranty/Service:

Contractor shall warrant that the Equipment will be free from material defects for a period of twelve (12) months from the date of acceptance. All service performed on this equipment shall be provided by certified and factory trained technicians. Contractor shall provide any required replacement components due to malfunctions on-site.