NIH-NICHD-OD-2018-4_Amendment_1_NCS_Repository_.pdf

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT 1

1. CONTRACT ID CODE

PAGE OF PAGES

N/A

2. AMENDMENT/MODIFICATION NUMBER

3 EFFECTIVE DATE

06/27/2018

4 REQUISITION/PURCHASE REQUISITION NUMBER , 5. PROJECT NUMBER (If applicable) N/A N/A

6. ISSUED BY CODE 7. ADMINISTERED BY (If other than Item 6) CODE I Office of Acquisitions Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health 6710B Rockledge Dr., First Floor, MSC 7000 Bethesda, Maryland 20892

8. NAME AND ADDRESS OF CONTRACTOR (Number, street, county, State and ZIP Code) (X) 9A. AMENDMENT OF SOLICITATION NUMBER

X

TO THE PROSPECTIVE OFFEROR

NIH-NICHD-OD-2018-4

9B. DATED (SEE ITEM 11)

10A. MODIFICATION OF CONTRACT/ORDER NUMBER

10B. DATED (SEE ITEM 13)

CODE I FACILITY CODE

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

[gj The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers D is extended. [gj is not extended.

Offers must acknowledge receipt of this amendment at the hour and date specified in the solicitation or as amended, by one of the following methods:

(a) By completing items 8 and 15, and returning one ( l ) copy of the amendment; and with the offer submitted, (b) By acknowledging receipt of this amendment on each copy of the offer submitted; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

12. ACCOUNTING AND APPROPRIATION DATA (If required)

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS.

IT MODIFIES THE CONTRACT/ORDER NUMBER AS DESCRIBED IN ITEM 14.

CHECK ONE A, THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT ORDER

NUMBER IN ITEM 10A,

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority)

E. IMPORT ANT: Contractor D is not [gj is required to sign this document and return ___ 1 copies to the issuing office.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

PURPOSE: To amend RFP NIH-NICHD-OD-2018-4, entitled, "National Children's Study (NCS) Biological and Environmental Sample Repository" - 1.) By listing prospective offerers' questions and responses to the questions and 2.) attaching the Inventory by Sample Type chart and the Sample Characterization Information chart.

DUE DATE: Until July 12, 2018, 3:00 PM Local Time (Eastern Standard Time (EST) (Unchanged).

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 1 0A, as heretofore changed, remains unchanged and in full force and effect.

15A. NAME AND TITLE OF SIGNER (Type or print)

15B. CONTRACTORIOFFEROR 15C. DATE SIGNED

(Signature of person authorized to sign)

Previous edition unusable

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)

16B. UNITED STATES OF AMERICA 16C. DATE SIGNED

(Signature of Contracting Officer)

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

1 18

Jacquelin Jones

June l3, 20l8

June 27, 2018

RFP No.: NIH-NICHD-OD-2018-4

June 27, 2018 1

National Children’s Study (NCS) Biological and Environmental Sample Repository

RFP Solicitation Number: NIH-NICHD-OD-2018-4

Questions and Responses

1) “Formatting for proposals” – Does the font size of the text in the tables and exhibits in the response also have to be ≥ 11?

Response: Yes, the font sizes should be consistent for input into the eCPS unless they are part of a .pdf document.

2) Technical Requirement # 2 “ Provide adequate storage facilities, refrigerators, mechanical freezers, and vapor-phase liquid nitrogen Dewars (VP-LN2) to cover a temperature range including ambient, +2 to +8 °C, -20°C, - 70 to -90 °C, and -130 to -150°C equipment to store the listed biological specimens. Wherever possible, maximize the number of frozen samples stored in vapor-phase liquid nitrogen for maximum long-term stability.”

Can the government provide the list of biological specimen types that are planned to be stored in VP - LN2?

Response: Sample types stored at vapor-phase liquid nitrogen temperature include blood and derivatives (whole blood with and without additives, plasma, serum, buffy coat, red blood cells, mixed buffy coat and red blood cells), saliva, breast milk, infant formula, urine (with and without additives), and vaginal swabs. The various sample types, aliquot volumes, and storage temperatures are described in the attached table from the National Children’s Study Archive Study Description and Guide document available on the NCS Archive website.

3) Technical Requirement # 9 “Locate, pull and ship up to 3,500 specimens up to 10 times per year.”

Please clarify if these are paid for by the contract or paid for in advance by the recipient investigators?

Response: Offerors should include the cost to pull and ship this volume of samples in the proposed budget.

4) Attachment 3 - SOW, Section I Background and Introduction, paragraph three - there is a statement that the contractor developed and implemented procedures to minimize variability in specimen handling. Paragraph five indicates that it is not anticipated that new material will be added to the NCS repository collection.

Question: Should the contractor anticipate creating additional samples through aliquoting? If so, is an annual estimate by material type available?

Response: No significant amount of additional sample processing is anticipated at this time.

Question: Is it possible to elaborate on the term “central processing” in the context of this SOW. If applicable, is an annual estimate by processing type available?

https://ncsarchive.s-3.net/static/data/NCS_Archive_Study_Description%20and%20User's%20Manual_July%202017.pdf https://ncsarchive.s-3.net/static/data/NCS_Archive_Study_Description%20and%20User's%20Manual_July%202017.pdf https://ncsarchive.s-3.net/

RFP No.: NIH-NICHD-OD-2018-4

June 27, 2018 2

Response: Central processing in that context refers to the procedures by which the specimens in the current collection were created, and not to anticipated future work.

5) Attachment 3 - SOW, Section I Background and Introduction, paragraph five - there is a statement that recipient investigators and institutions are expected to bear the cost of sample selection and shipment and these costs are negotiated between the recipient/investigator and the contractor.

Question: If available will the government provide the contractor with a current services price list?

Response: A current services price list is not available.

Question: Are costs associated with these services collected by individual payment negotiated directly with the recipient/investigator or as a pass-through cost collected by the government?

Response: Any such costs are to be negotiated directly between the recipient and the repository.

6) Attachment 3 - SOW, Section I Background and Introduction, paragraph six - there is a statement that the collection consists of approximately 250,000 samples. The Technical Requirements section bullet ten lists 30,000 “posterity” samples.

Question: Please provide a breakdown by storage type of the 250,000 samples.

Response: See the table noted in the response to question #2 and the attached table “NCS Archive Biological and Environmental Sample Inventory – June 2018”.

Question: Does the 250,000 include the 30,000 “posterity” samples?

Response: Yes, the 250,000 samples includes the 30,000 “posterity” samples.

7) Attachment 3 - SOW, Section III Technical Requirements, page five, bullet four - there is a statement that calls for a final transition plan for the time if transfer or destruction of the collection. There does not appear to be any reference to the transfer in of the collection from the incumbent.

Question: Please provide information on the responsibilities of the incumbent to transition to a new contractor, related to the cost and logistics of sample transfer.

Response: The incumbent is required to provide a final transition plan that details the secure transition of all contract related materials to a possible successor contractor, with materials organized and catalogued in sufficient detail to support an orderly transition to a possible successor contractor.

NCS Archive Biological and Environmental Sample Inventory – June 2018

Sample Type Number of

Samples

Air - Filter 337

Air - PM Filter 1

Blood + 1% Ascorbic 3,831

Blood + RPMI/DMSO 4,175

Breast Milk 4,186

Breast Milk + PCA 268

Buffy Coat 944

Buffy Coat + RBC 5,530

DNA 14

DNA 200ng/ul 10

DNA Stock Vial 2,702

DNA, 200 ng/uL 4,680

Dust 12

Dust - Inorganics 523

Dust - Organics 784

Dust - Vacuum - Allergens 823

Dust - Vacuum Bag 91

Dust - Wipe Inorganic 267

Dust - Wipe Pyrethroids 284

Dust - Wipe SVOC 16

Dust (non-NCS) 157

Dust- Wipe Organic 1

Formula 133

Hair 1,075

HPLC Water 2,619

Meconium 454

Membrane 40

Nails 842

Placenta 3,364

Plasma 58,388

RBC 473

RNA 8

RNA 25ng/ul 478

RNA Stock Vial 569

Saliva 6,197

Serum 33,520

Serum + MPA 482

Umbilical Cord 362

Urine 64,674

Urine (non-NCS) 165

Urine + preservative 10

Urine + Sulfamic Acid 3,871

Vaginal Slide 1,304

Vaginal Swab 2,411

Water - Pharma TWF 854

Water- Pest - TWQ 546

Whole Blood 14,431

Whole Blood Spot 11,252

SAMPLE CHARACTERIZATION INFORMATION

Characterization Information for NCS Repository Samples with Inventory Counts of 10 or Greater as of

Biological Samples

Primary Sample

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Blood Pre-birth T3M, T1F, PV1 ACD Tube AD10 8.5mL ambient Whole Blood with 20%

DMSO/RPMI

2.0mL VPLN IVS , ARS Mother, Father

Saliva Post-birth 36M Adult Saliva-Salimetrics swab AS30 Frozen Saliva 0.5mL -80°C ARS Adult

Blood Birth Birth Child Blood Spot Card (Protein Saver

Card) B009 75uL per spot Frozen

Whole Blood (903 Protein

Saver)

1 drop of blood per spot from heel stick

25°C IVS Child

Blood Birth Birth Child Blood Spot Card (FTA Card) B010 75uL per spot Ambient Whole Blood

(FTA)

1 drop of blood per spot from heel stick

-20°C IVS Child

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM01 120mL capacity Frozen Breast Milk

0.2mL + 0.6mL 4% perchloric acid VPLN IVS Mother

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM01 120mL capacity Frozen Breast Milk Various, 0.5-

5mL VPLN IVS Mother

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM01 120mL capacity Frozen Breast Milk 4mL

(prescreened) VPLN IVS Mother

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM01 120mL capacity Frozen Breast Milk 10mL

(prescreened) VPLN IVS Mother

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM01 120mL capacity Frozen Breast Milk 10mL

(prescreened) VPLN IVS Mother

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM20 120mL capacity Frozen Breast Milk

0.2mL + 0.6mL 4% perchloric acid VPLN ARS Mother

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM20 120mL capacity Frozen Breast Milk Various, 0.5-

5mL VPLN ARS Mother

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM20 120mL capacity Frozen Breast Milk 4mL

(prescreened) VPLN ARS Mother

Breast milk Post-birth 1M, 3M Mother Urine Collection Container BM20 120mL capacity Frozen Breast Milk 10mL

(prescreened) VPLN ARS Mother

Primary Sample

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Urine Post-birth 6M Child Urine Collection Container BU01 120mL capacity Frozen Urine

Various, 0.5- 8mL

(prescreened) VPLN IVS Child

Urine Post-birth 6M, 12M, 36M Child

Urine Collection Container BU20 120mL capacity Frozen Urine

Various, 0.5- 8mL

(prescreened) VPLN ARS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C Plasma Various, 0.5- 1.5mL VPLN IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C Plasma 4mL (prescreened) VPLN IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C Whole blood 0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C Whole blood 1mL (prescreened) VPLN IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C Whole blood 2.0mL (prescreened) VPLN IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C Whole blood 0.5mL VPLN IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C Buffy coat+ RBCs 2.5mL VPLN IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C 903 Blood spot cards

0.075mL per spot -20°C IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag-

CPD

CB01 210mL capacity 2 - 8°C FTA Blood spot cards

0.075mL per spot 25°C IVS Child

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C plasma Various, 0.5- 1.5mL VPLN ARS Child

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C plasma 4mL VPLN ARS Child

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C Whole blood 0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN ARS Child

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C Whole blood 1mL (prescreened) VPLN ARS Child

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C Whole blood 2.0mL (prescreened) VPLN ARS Child

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C Whole blood 0.5mL VPLN ARS Child

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C Buffy coat+ RBCs 2.5mL VPLN ARS Child

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C 903 Blood spot cards

0.075mL per spot -20°C ARS Child

Blood Birth Birth Child Cord Blood

Collection Bag- Heparin

CB11 210mL capacity 2 - 8°C FTA Blood spot cards

0.075mL per spot 25°C ARS Child

Blood Birth PBS Birth Child

Cord blood- Lavender Top Tube CB17 6-10mL 2 - 8°C plasma 0.5mL VPLN PBS Child

Blood Birth PBS Birth Child

Cord blood- Lavender Top Tube CB17 6-10mL 2 - 8°C Whole blood

0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN PBS Child

Blood Birth PBS Birth Child

Cord blood- Lavender Top Tube CB17 6-10mL 2 - 8°C Whole blood 0.2mL VPLN PBS Child

Blood Birth PBS Birth Child

Cord blood- Lavender Top Tube CB17 6mL 2 - 8°C Buffy coat+

RBCs 2.5mL VPLN PBS Child

Blood Birth PBS Birth Child

Cord blood- Lavender Top Tube CB17 6-10mL 2 - 8°C Buffy coat 1.25mL VPLN PBS Child

Blood Birth PBS Birth Child

Cord blood- Lavender Top Tube CB17 6-10mL 2 - 8°C RBCs 1.25mL VPLN PBS Child

Blood Birth PBS Birth Child

Cord blood- Lavender Top Tube CB17 6-10mL 2 - 8°C 903 Blood spot cards 0.075mL per spot -20°C PBS Child

Blood Birth PBS Birth Child

Cord blood- Lavender Top Tube CB17 6-10mL 2 - 8°C FTA Blood spot cards 0.075mL per spot 25°C PBS Child

Blood Birth PBS Birth Child

Cord blood- Red Top Tube CB18 10 mL 2 - 8°C Serum 0.5mL VPLN PBS Child

Blood Birth PBS Birth Child

Cord blood- Red Top Tube CB18 10 mL 2 - 8°C Serum 0.5mL

(amber) VPLN PBS Child

Blood Birth Birth Child Cord Stick Lavender Top Tube CL10 6-10mL 2 - 8°C Plasma 0.5mL VPLN ARS Child

Blood Birth Birth Child Cord Stick Lavender Top Tube CL10 6-10mL 2 - 8°C Whole blood

0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN ARS Child

Blood Birth Birth Child Cord Stick Lavender Top Tube CL10 6-10mL 2 - 8°C Whole blood 0.2mL VPLN ARS Child

Blood Birth Birth Child Cord Stick Lavender Top Tube CL10 6-10mL 2 - 8°C Buffy coat+

RBCs 2.5mL VPLN ARS Child

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Blood Birth Birth Child Cord Stick Lavender Top Tube CL10 6-10mL 2 - 8°C 903 Blood spot cards 0.075mL per spot -20°C ARS Child

Blood Birth Birth Child Cord Stick Lavender Top Tube CL10 6-10mL 2 - 8°C FTA Blood spot cards 0.075mL per spot 25°C ARS Child

Saliva Post-birth 12M Child Saliva-Oragene (1.5ml) CS20 1.5mL Ambient Saliva 0.75mL -20°C ARS Child

Saliva Post-birth 12M Child Saliva-Oragene (1.5ml) CS20 1.5mL Ambient Saliva 0.75mL -20°C ARS Child

Saliva Post-birth 36M Child Saliva-Salimetrics swab CS21 Frozen Saliva 0.5mL -80°C ARS Child

Saliva Post-birth 36M Child Saliva-Salimetrics swab CS21 Frozen Saliva 0.5mL -80°C ARS Child

Urine Post-birth 36M Child Urine Collection Container CU20 120mL capacity Frozen Urine

1.8mL + 20uL sulfamic acid (prescreened)

VPLN ARS Child

Urine Post-birth 36M Child Urine Collection Container CU20 120mL capacity Frozen Urine

Various, 0.5- 8mL

(prescreened) VPLN ARS Child

Infant Formula Birth Birth Mother - FM01 5mL Frozen Formula 5mL

(prescreened) VPLN IVS Mother

Hair Pre-birth P1M, T3M, T1F bag HR01 20 strands minimum Ambient Hair bag 25°C IVS Mother, Father

Blood Birth PBS Birth Child

Blood Spot Card (Protein Saver

Card) HS17 75uL per spot Frozen

Whole Blood (903 Protein

Saver)

1 drop of blood per spot from heel stick

-20°C PBS Child

Blood Pre-birth P1M, T1M, BR-M, T1F

Lavender Top Tube (prescreened) LP10 3mL Frozen Whole Blood None -20°C IVS , ARS Mother, Father

Blood Post-birth 36M Child Lavender Top Tube (prescreened) LP20 3mL Frozen Whole Blood None -20°C ARS Child

Blood Post-birth 36M Adult Lavender Top Tube (prescreened) LP40 3mL Frozen Whole Blood None -20°C ARS Adult

Blood Pre-birth P1M, T1M, T3M, Pre-preg, PV1, PV2

Lavender Top Tube LV10 6mL 2 - 8°C Plasma 0.5mL VPLN IVS , ARS Mother

Blood Pre-birth P1M, T1M, T3M, Pre-preg, PV1, PV2

Lavender Top Tube LV10 6mL 2 - 8°C Whole blood 0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN IVS , ARS Mother

Blood Pre-birth P1M, T1M, T3M, Pre-preg, PV1, PV2

Lavender Top Tube LV10 6mL 2 - 8°C Whole blood 0.2mL VPLN IVS , ARS Mother

Blood Pre-birth P1M, T1M, T3M, Pre-preg, PV1, PV2

Lavender Top Tube LV10 6mL 2 - 8°C Buffy coat+ RBCs 2.5mL VPLN IVS , ARS Mother

Blood Pre-birth P1M, T1M, T3M, Pre-preg, PV1, PV2)

Lavender Top Tube LV10 6mL 2 - 8°C 903 Blood spot cards

0.075mL per spot -20°C IVS , ARS Mother

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Blood Pre-birth P1M, T1M, T3M, Pre-preg, PV1, PV2

Lavender Top Tube LV10 6mL 2 - 8°C FTA Blood spot cards

0.075mL per spot 25°C IVS , ARS Mother

Blood Pre-birth T1F Lavender Top Tube LV11 6mL 2 - 8°C Plasma 0.5mL VPLN IVS Father

Blood Pre-birth T1F Lavender Top Tube LV11 6mL 2 - 8°C Buffy coat+ RBCs 2.5mL VPLN IVS Father

Blood Pre-birth T1F Lavender Top Tube LV11 6mL 2 - 8°C 903 Blood spot cards

0.075mL per spot -20°C IVS Father

Blood Pre-birth T1F Lavender Top Tube LV11 6mL 2 - 8°C FTA Blood spot cards

0.075mL per spot 25°C IVS Father

Blood Birth PBS Birth Mother Lavender Top Tube LV17 6mL 2 - 8°C Plasma 0.5mL VPLN PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV17 6mL 2 - 8°C Whole blood

0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV17 6mL 2 - 8°C Buffy coat 1.25mL VPLN PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV17 6mL 2 - 8°C RBCs 1.25mL VPLN PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV17 6mL 2 - 8°C 903 Blood spot cards 0.075mL per spot -20°C PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV17 6mL 2 - 8°C FTA Blood spot cards 1 drop of blood per spot from heel stick

25°C PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV18 6mL 2 - 8°C Plasma 0.5mL VPLN PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV18 6mL 2 - 8°C Whole blood 0.2mL VPLN PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV18 6mL 2 - 8°C Buffy coat 1.25mL VPLN PBS Mother

Blood Birth PBS Birth Mother Lavender Top Tube LV18 6mL 2 - 8°C RBCs 1.25mL VPLN PBS Mother

Blood Post-birth 12M Child Lavender Top Tube LV21 3mL 2 - 8°C Plasma 0.5mL VPLN ARS Child

Blood Post-birth 12M Child Lavender Top Tube LV21 3mL 2 - 8°C Whole blood 0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN ARS Child

Blood Post-birth 12M Child Lavender Top Tube LV21 3mL 2 - 8°C Whole blood 0.2mL VPLN ARS Child Blood Post-birth 12M Child Lavender Top Tube LV21 3mL 2 - 8°C Buffy coat 0.25mL VPLN ARS Child Blood Post-birth 12M Child Lavender Top Tube LV21 3mL 2 - 8°C RBCs 0.25mL VPLN ARS Child

Blood Post-birth 12M Child Lavender Top Tube LV21 3mL 2 - 8°C 903 Blood spot cards

0.075mL per spot -20°C ARS Child

Blood Post-birth 12M Child Lavender Top Tube LV21 3mL 2 - 8°C FTA Blood spot cards

0.075mL per spot 25°C ARS Child

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Blood Post-birth 36M Child Lavender Top Tube LV22 4mL 2 - 8°C Plasma 0.5mL VPLN ARS Child

Blood Post-birth 36M Child Lavender Top Tube LV22 4mL 2 - 8°C Whole Blood 0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN ARS Child

Blood Post-birth 36M Child Lavender Top Tube LV22 4mL 2 - 8°C Whole Blood 0.25mL VPLN ARS Child Blood Post-birth 36M Child Lavender Top Tube LV22 4mL 2 - 8°C Buffy coat 0.25mL VPLN ARS Child Blood Post-birth 36M Child Lavender Top Tube LV22 4mL 2 - 8°C RBCs 0.75mL VPLN ARS Child

Blood Post-birth 36M Child Lavender Top Tube LV22 3mL 2 - 8°C 903 Blood spot cards

0.075mL per spot -20°C ARS Child

Blood Post-birth 36M Child Lavender Top Tube LV22 3mL 2 - 8°C FTA Blood spot cards

0.075mL per spot 25°C ARS Child

Blood Post-birth 36M Adult Lavender Top Tube LV30 6mL 2 - 8°C Plasma 0.5mL VPLN ARS Adult

Blood Post-birth 36M Adult Lavender Top Tube LV30 6mL 2 - 8°C Whole Blood 0.1mL + 1mL 1% Ascorbic Acid (amber)

VPLN ARS Adult

Blood Post-birth 36M Adult Lavender Top Tube LV30 6mL 2 - 8°C Whole Blood 1.0mL VPLN ARS Adult Blood Post-birth 36M Adult Lavender Top Tube LV30 6mL 2 - 8°C Buffy coat 0.5mL VPLN ARS Adult Blood Post-birth 36M Adult Lavender Top Tube LV30 6mL 2 - 8°C RBCs 1.25mL VPLN ARS Adult

Blood Post-birth 36M Adult Lavender Top Tube LV30 6mL 2 - 8°C 903 Blood spot cards

0.075mL per spot -20°C ARS Adult

Blood Post-birth 36M Adult Lavender Top Tube LV30 6mL 2 - 8°C FTA Blood spot cards

0.075mL per spot 25°C ARS Adult

Meconium Birth Birth Child various volume in 30mL para-pak container MC01 30mL capacity Frozen Meconium various volume in

30mL para-pak container

-80°C IVS Child

Nails Pre-birth T3M, T1F bag NL01 nails Ambient Toe Nails bag 25°C IVS Mother, Father

Placenta (cord) Birth Birth Mother Placental Tissue

Sample PC01 placenta Fresh Tissue -

RefrigeratedFixe d Tissue - Ambient

Umbilical Cord Block/Slide 25°C IVS Mother

Placenta (membrane

Birth Birth Mother Placental Tissue

Sample PC01 placenta

Fresh Tissue - Refrigerated

Fixed Tissue - Ambient

Membrane Block/Slide 25°C IVS Mother

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Placenta Birth Birth Mother Placental Tissue Sample PC01 placenta

Fresh Tissue - Refrigerated

Fixed Tissue - Ambient

Placenta Block/Slide 25°C IVS Mother

Placenta Birth PBS Birth Mother

Placental Tissue Sample PC17 5ml RNALater 2 - 8°C Placenta None -80°C PBS Mother

Placenta Birth PBS Birth Mother

Placental Tissue Sample PC18 5ml RNALater 2 - 8°C Placenta None -80°C PBS Mother

Blood Pre-birth T1M, T3M, Pre-preg, PV1 P100 Tube PN10 8.5mL 2 - 8°C Plasma 0.5mL VPLN IVS , ARS Mother

Blood Pre-birth P1M, T1M, T3M, PV1, PV2

Plasma Preparation

Tube PP10 5mL 2 - 8°C Plasma 0.5mL VPLN IVS , ARS Mother

Blood Pre-birth T3M, PV2 PaxGene Tube PX10 2.5mL Frozen Whole blood None -80°C IVS , ARS Mother Blood Post-birth 36M Child PaxGene Tube PX20 2.5mL Frozen Whole blood None -80°C ARS Child Blood Post-birth 36M Adult PaxGene Tube PX30 2.5mL Frozen Whole blood None -80°C ARS Adult

Blood Pre-birth PV2 Royal blue EDTA serum tube RB10 6mL Frozen Whole blood None -80°C ARS Mother

Blood Pre-birth P1M, T1M, Pre-preg, PV1, PV2

Red Top Tube RD10 10mL 2 - 8°C Serum 0.5mL VPLN IVS , ARS Mother

Blood Pre-birth P1M, T1M, Pre-preg, PV1, PV2

Red Top Tube RD10 10mL 2 - 8°C Serum 0.5mL (amber) VPLN IVS , ARS Mother

Blood Pre-birth P1M, T1M, T3M Red Top Tube RD11 10mL 2 - 8°C Serum 5mL

(prescreened) VPLN IVS Mother

Blood Pre-birth T1F Red Top Tube RD12 10mL 2 - 8°C Serum 0.5mL VPLN IVS Father

Blood Pre-birth T1F Red Top Tube RD12 10mL 2 - 8°C Serum 4.5mL (prescreened) VPLN IVS Father

Blood Pre-birth T3M Red Top Tube RD13 10mL 2 - 8°C Serum 0.25mL + 1mL MPA (amber) VPLN IVS Mother

Blood Pre-birth T3M Red Top Tube RD13 10mL 2 - 8°C Serum 0.5mL (amber) VPLN IVS Mother

Blood Pre-birth T3M Red Top Tube RD13 10mL 2 - 8°C Serum 0.5mL VPLN IVS Mother

Blood Pre-birth T3M Red Top Tube RD13 10mL 2 - 8°C Serum 1mL (prescreened) VPLN IVS Mother

Blood Birth Birth Mother Red Top Tube RD14 10mL 2 - 8°C Serum 5mL (prescreened) VPLN IVS Mother

Blood Birth Birth Mother Red Top Tube RD15 10mL 2 - 8°C Serum 0.5mL VPLN IVS Mother

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Blood Birth Birth Mother Red Top Tube RD15 10mL 2 - 8°C Serum 4.5mL (prescreened) VPLN IVS Mother

Blood Birth Birth Mother Red Top Tube RD16 10mL 2 - 8°C Serum 5mL (prescreened) VPLN IVS Mother

Blood Birth PBS Birth Mother Red Top Tube RD17 10mL 2 - 8°C Serum 0.5mL VPLN PBS Mother

Blood Birth PBS Birth Mother Red Top Tube RD18 10mL 2 - 8°C Serum 0.5mL VPLN PBS Mother

Blood Birth PBS Birth Mother Red Top Tube RD18 10mL 2 - 8°C Serum 4.5mL

(prescreened) VPLN PBS Mother

Blood Post-birth 12M Child Red Top Tube RD20 3mL 2 - 8°C Serum 0.5mL VPLN ARS Child

Blood Post-birth 12M Child Red Top Tube RD21 3mL 2 - 8°C Serum 0.5mL (amber) VPLN ARS Child

Blood Post-birth 12M Child Red Top Tube RD21 3mL 2 - 8°C Serum 0.5mL VPLN ARS Child

Blood Post-birth 36M Child Red Top Tube RD22 5mL 2 - 8°C Serum 0.25mL + 1mL MPA (amber) VPLN ARS Child

Blood Post-birth 36M Child Red Top Tube RD22 5mL 2 - 8°C Serum 0.5mL (amber) VPLN ARS Child

Blood Post-birth 36M Child Red Top Tube RD22 5mL 2 - 8°C Serum 0.5mL VPLN ARS Child

Blood Post-birth 36M Adult Red Top Tube RD30 10mL 2 - 8°C Serum 0.25mL + 1mL MPA (amber) VPLN ARS Adult

Blood Post-birth 36M Adult Red Top Tube RD30 10mL 2 - 8°C Serum 1mL VPLN ARS Adult Blood Post-birth 36M Adult Red Top Tube RD30 10mL 2 - 8°C Serum 0.5mL VPLN ARS Adult

Saliva Pre-birth P1M,T1M, T3M,T1F Saliva-Oragene SB01 2mL Ambient Saliva 2mL -20°C IVS Mother, Father

Saliva Pre-birth P1M,T1M, T3M,T1F Saliva-Oragene SB01 2mL Ambient Saliva 2mL -20°C IVS Mother, Father

Saliva Pre-birth T1M, T3M 2mL cryovial SL01 2mL Frozen Saliva 2mL VPLN IVS Mother Saliva Pre-birth T1M, T3M 2mL cryovial SL02 2mL Frozen Saliva 2mL VPLN IVS Mother Saliva Pre-birth T1M, T3M 2mL cryovial SL03 2mL Frozen Saliva 2mL VPLN IVS Mother Saliva Pre-birth T1M, T3M 2mL cryovial SL04 2mL Frozen Saliva 2mL VPLN IVS Mother Saliva Pre-birth T1M, T3M 2mL cryovial SL05 2mL Frozen Saliva 2mL VPLN IVS Mother Saliva Pre-birth T1M, T3M 2mL cryovial SL06 2mL Frozen Saliva 2mL VPLN IVS Mother

Blood Pre-birth P1M, T1M, T3M, PV1, PV2

Serum Separator

Tube SS10 8.5mL 2 - 8°C Serum 0.5mL VPLN IVS , ARS Mother

Blood Post-birth 36M Child Serum Separator Tube SS20 3.5mL 2 - 8°C Serum 0.5mL VPLN ARS Child

Blood Post-birth 36M Adult Serum Separator Tube SS30 8.5mL 2 - 8°C Serum 0.5mL VPLN ARS Adult

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Urine Pre-birth T1M Urine Collection Container

UR01 (old aliquoting extensions) Aliquoting extensions updated May

120mL capacity Frozen Urine 1mL VPLN IVS Mother

Urine Pre-birth T1M Urine Collection Container

UR01 (old aliquoting extensions) Aliquoting extensions updated May

120mL capacity Frozen Urine 1mL (prescreened) VPLN IVS Mother

Urine Pre-birth T1M Urine Collection Container

UR01 (old aliquoting extensions) Aliquoting extensions updated May

120mL capacity Frozen Urine 2mL (prescreened) VPLN IVS Mother

Urine Pre-birth P1M, T1M, T3M, T1F, Pre-preg, PV2

Urine Collection Container

UR01,

UR02, PU01 120mL capacity Frozen Urine

1.8mL + 20uL sulfamic acid (prescreened)

VPLN

IVS

(UR01,

UR02,

PU01),

ARS

(UR01)

Mother, Father

Urine Pre-birth P1M, T1M, T3M, T1F, Pre-preg, PV2

Urine Collection Container

UR01,

UR02, PU01 120mL capacity Frozen Urine 2.0mL

(prescreened) VPLN

IVS

(UR01,

UR02,

PU01),

ARS

(UR01)

Mother, Father

Urine Pre-birth P1M, T1M, T3M, T1F, Pre-preg, PV2

Urine Collection Container

UR01,

UR02, PU01 120mL capacity Frozen Urine Various, 0.5-

5mL VPLN

IVS(UR01

, UR02,

PU01),

ARS

(UR01)

Mother, Father

Urine Pre-birth P1M, T1M, T3M, T1F, Pre-preg, PV2

Urine Collection Container

UR01,

UR02, PU01 120mL capacity Frozen Urine 8mL

(prescreened) VPLN

IVS

(UR01,

UR02,

PU01),

ARS

(UR01)

Mother, Father

Urine Birth PBS Birth Mother

Urine Collection Container UR17 120mL capacity Frozen Urine

1.8mL + 20uL sulfamic acid (prescreened)

VPLN PBS Mother

Type

Timing Relative to Birth

Visit Type Collection Type/Container

Parent Sample ID Extension

Draw/Collection Volume

Temperature at Receipt

Processed Component

Aliquot Volume or Quantity

Storage Temperature

Study Phase

Participant Type

Urine Birth PBS Birth Mother

Urine Collection Container UR17 120mL capacity Frozen Urine 8mL

(prescreened) VPLN PBS Mother

Urine Birth PBS Birth Mother

Urine Collection Container UR17 120mL capacity Frozen Urine 2.0mL

(prescreened) VPLN PBS Mother

Urine Birth PBS Birth Mother

Urine Collection Container UR17 120mL capacity Frozen Urine Various, 0.5-

5mL VPLN PBS Mother

Urine Post-birth 36M Adult Urine Collection Container UR30 120mL capacity Frozen Urine

1.8mL + 20uL sulfamic acid (prescreened)

VPLN ARS Adult

Urine Post-birth 36M Adult Urine Collection Container UR30 120mL capacity Frozen Urine 8mL

(prescreened) VPLN ARS Adult

Urine Post-birth 36M Adult Urine Collection Container UR30 120mL capacity Frozen Urine 2.0mL

(prescreened) VPLN ARS Adult

Urine Post-birth 36M Adult Urine Collection Container UR30 120mL capacity Frozen Urine Various, 0.5-

5mL VPLN ARS Adult

Slide, vaginal Pre-birth P1M, T1M, T3M Vaginal Slide

Sample VL01 slide Ambient Vaginal Slide Slide 25°C IVS Mother

Swab, vaginal Pre-birth P1M, T1M, T3M 5mL cryovial VS01 swab in 5mL vial Frozen Vaginal Swab 1.0mL VPLN IVS Mother

Swab, vaginal Pre-birth P1M, T1M, T3M 5mL cryovial VS02 swab in 5mL vial Frozen Vaginal Swab 1.0mL VPLN IVS Mother

DNA Birth PBS Birth Mother 2mL cryovial PC17 DNA extract from placenta Frozen DNA- 200 ng/ul 50 ul -80°C PBS Mother

DNA

P1M, T1M, T3M, Pre-preg, PV1, PV2, T1F, Birth

Child

2mL cryovial 7001, 7021 RNA extract from mixed buffy coat Frozen DNA- 200 ng/ul 50 ul -80°C IVS Mother, Father, Child

RNA Pre-birth T3M, PV2 2mL cryovial PX10 RNA extract from whole blood Frozen RNA- 25 ng/ul 40 ul -80°C IVS , ARS Mother, Father

RNA Birth PBS Birth Mother 2mL cryovial PC17 RNA extract from placenta Frozen RNA- 25 ng/ul 40 ul -80°C PBS Mother

Abbreviations: 12M=12 months; 1M=1 month; 36M=36 months; 3M=3 months; 6M=6 months; ARS=Alternate Recruitment Substudy; BR-M=Birth-mother;

CPD=citrate-phosphate-dextrose; DMSO=Dimethyl sulfoxide; EDTA=ethylene diamine tetraacetate; FTA=Flinders Technology Associates; HDPE=High-density polyethylene; IVS=Initial Vanguard Study; MPA= Metaphosphoric acid; P1M=maternal prepregnancy visit in IVS; PBS=Provider-Based Sampling Substudy;

y visit in ARS; RPMI=Roswell Park Memorial Institute growth medium; SVOC=semi-volatile organic compounds; T1F=first trimester father visit in IVS; T1M=first trimester mother visit in IVS;

T3M=third trimester mother visit in IVS; VPLN=vapor phase liquid nitrogen

Characterization Information for NCS Repository Samples with Inventory Counts of 10 or Greater as of

Environmental Samples

Primary Sample Type

Timing Relative to Birth

Visit Type

Collection Type/Con tainer

Parent Sample ID Extension

Draw/Collection Volume Processed Component

Aliquot Volume or Quantity

Storage Temperature Study Phase Participant Type

Air – PM2.5 filter Pre-birth P1M Filter Disk AP01 Air - PM Filter 37 mm Teflon filter in sealed container, bagged Not Applicable Walk-in -20 C IVS Not applicable

Dust – Vacuum Allergens/Endoto xin Pre-birth T1M, T3M 15 mL vial DA01 Dust - Vacuum - Allergens

Dustream filter in 15 ml vial, double bagged Not Applicable -80°C IVS Not applicable

Dust – Vacuum Bag; Processed remainder Pre-birth PV1 Amber Jar DB02 Dust - Vacuum Bag

Sieved dust in 2 oz or larger amber glass jar with Teflon lined cap, bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Organics Pre-birth PV1 Amber Jar DB20 Dust - Organics Sieved dust in 30 ml amber glass jar, double bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Organics Pre-birth PV1 Amber Jar DB21 Dust - Organics Sieved dust in 30 ml amber glass jar, double bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Organics Pre-birth PV1 Amber Jar DB22 Dust - Organics Sieved dust in 30 ml amber glass jar, double bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Organics Pre-birth PV1 Amber Jar DB23 Dust - Organics Sieved dust in 30 ml amber glass jar, double bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Organics Pre-birth PV1 Amber Jar DB24 Dust - Organics Sieved dust in 30 ml amber glass jar, double bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Inorganics Pre-birth PV1 10 mL vial DB25 Dust - Inorganics

Sieved dust in 10 ml polyethylene vial, double bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Inorganics Pre-birth PV1 10 mL vial DB26 Dust - Inorganics

Sieved dust in 10 ml polyethylene vial, double bagged 1 -80°C ARS Not applicable

Sample Type

Timing Relative to Birth

Visit Type

Collection Type/Con tainer

Parent Sample ID Extension

Draw/Collection Volume Processed Component

Aliquot Volume or Quantity

Storage Temperature Study Phase Participant Type

Dust – Vacuum Bag Aliquot --

Inorganics Pre-birth PV1 10 mL vial DB27 Dust - Inorganics

Sieved dust in 10 ml polyethylene vial, double bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Inorganics Pre-birth PV1 10 mL vial DB28 Dust - Inorganics

Sieved dust in 10 ml polyethylene vial, double bagged 1 -80°C ARS Not applicable

Dust – Vacuum Bag Aliquot --

Inorganics Pre-birth PV1 10 mL vial DB29 Dust - Inorganics

Sieved dust in 10 ml polyethylene vial, double bagged 1 -80°C ARS Not applicable

Dust – Wipe Inorganic Pre-birth T1M, T3M 2 oz Amber

Jar DN01 Dust - Wipe Inorganic

Wipe in 2 oz amber glass jar with Teflon lined cap, bagged Not Applicable -20°C IVS Not applicable

Dust – Wipe Pyrethroids Pre-birth

P1M, T1M, T3M

2 oz Amber Jar DP02 Dust - Wipe

Pyrethroids Wipe in 2 oz amber glass jar with Teflon lined cap, double bagged Not Applicable -80°C IVS Not applicable

Dust – Wipe SVOC Pre-birth T1M, T3M 2 oz Amber

Jar DS01 Dust - Wipe SVOC Wipe in 2 oz amber glass jar with Teflon lined cap, double bagged Not Applicable -80°C IVS Not applicable

Dust – Vacuum Bag Aliquot --

Organics Pre-birth PV1 Amber Jar SB20 Dust - Organics Sieved dust in 30 ml amber glass jar, double bagged 1 -80°C ARS Not applicable

Dust – Deposition Plate Wipe - Inorganics

Pre-birth T3M Amber Jar SD08 Dust - Wipe Inorganic

Wipe in 2 oz amber glass jar with Teflon lined cap, double bagged Not Applicable -20°C IVS Not applicable

Water - Pharmaceuticals Pre-birth PV1 1 L amber

HDPE bottle SF01 Water - Pharma TWF Tap water with 50 mg of 1%

Ascorbic acid in 1 liter HDPE bottle in foam insert, bagged – 3 bottles/sample

1 L -20°C ARS Not applicable

Water - Pharmaceuticals Pre-birth PV1 1 L amber

HDPE bottle SF02 Water - Pharma TWF Tap water with 50 mg of 1%

Ascorbic acid in 1 liter HDPE bottle in foam insert, bagged – 3 bottles/sample

1 L -20°C ARS Not applicable

Water - Pharmaceuticals Pre-birth PV1 1 L amber

HDPE bottle SF03 Water - Pharma TWF Tap water with 50 mg of 1%

Ascorbic acid in 1 liter HDPE bottle in foam insert, bagged – 3 bottles/sample

1 L -20°C ARS Not applicable

Sample Type

Timing Relative to Birth

Visit Type

Collection Type/Con tainer

Parent Sample ID Extension

Draw/Collection Volume Processed Component

Aliquot Volume or Quantity

Storage Temperature Study Phase Participant Type

Water - Pesticides Pre-birth PV1 1 L amber

HDPE bottle SQ01 Water - Pest - TWQ

Tap water with 80 mg of sodium thiosulphate in 1 liter HDPE bottle in foam insert, bagged – 2 bottles/sample

1 L -20°C ARS Not applicable

Water - Pesticides Pre-birth PV1 1 L amber

HDPE bottle SQ02 Water - Pest - TWQ

Tap water with 80 mg of sodium thiosulphate in 1 liter HDPE bottle in foam insert, bagged – 2 bottles/sample

1 L -20°C ARS Not applicable

Water - Pharmaceuticals Pre-birth PV1 1 L amber

HDPE bottle WF01 Water - Pharma TWF Tap water with 50 mg of 1%

Ascorbic acid in 1 liter HDPE bottle in foam insert, bagged – 3 bottles/sample

1 L -20°C ARS Not applicable

Water - Pharmaceuticals Pre-birth PV1 1 L amber

HDPE bottle WF02 Water - Pharma TWF Tap water with 50 mg of 1%

Ascorbic acid in 1 liter HDPE bottle in foam insert, bagged – 3 bottles/sample

1 L -20°C ARS Not applicable

Water - Pharmaceuticals Pre-birth PV1 1 L amber

HDPE bottle WF03 Water - Pharma TWF Tap water with 50 mg of 1%

Ascorbic acid in 1 liter HDPE bottle in foam insert, bagged – 3 bottles/sample

1 L -20°C ARS Not applicable

Water - Pesticides Pre-birth PV1 1 L amber

HDPE bottle WQ01 Water - Pest - TWQ

Tap water with 80 mg of sodium thiosulphate in 1 liter HDPE bottle in foam insert, bagged – 2 bottles/sample

1 L -20°C ARS Not applicable

Water - Pesticides Pre-birth PV1 1 L amber

HDPE bottle WQ02 Water - Pest - TWQ

Tap water with 80 mg of sodium thiosulphate in 1 liter HDPE bottle in foam insert, bagged – 2 bottles/sample

1 L -20°C ARS Not Applicable

Abbreviations: 12M=12 months; 1M=1 month; 36M=36 months; 3M=3 months; 6M=6 months; ARS=Alternate Recruitment Substudy; BR-M=Birth-mother;

CPD=citrate-phosphate-dextrose; DMSO=Dimethyl sulfoxide; EDTA=ethylene diamine tetraacetate; FTA=Flinders Technology Associates; HDPE=High-density polyethylene; IVS=Initial Vanguard Study; MPA= Metaphosphoric acid; P1M=maternal prepregnancy visit in IVS; PBS=Provider-Based Sampling Substudy;

irst pregnancy visit in ARS; PV2=maternal second pregnancy visit in ARS; RPMI=Roswell Park Memorial Institute growth medium; SVOC=semi-volatile organic compounds; T1F=first trimester father visit in IVS; T1M=first trimester mother visit in IVS;

T3M=third trimester mother visit in IVS; VPLN=vapor phase liquid nitrogen