1._PD_-_Corrected.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The Division of Preclinical Innovation (DPI) within the National Center for Advancing Translational Sciences (NCATS) is requesting an Ultra Performance Liquid Chromatography (UPLC) system coupled with a Mass Spectrometry (MS) detector for use in the Medicinal Chemistry Lab.

2. Background Information and Objective:

Ultra-Performance Liquid Chromatography (UPLC) is a relatively new technique giving new possibilities in liquid chromatography, especially concerning decrease of time and solvent consumption. A UPLC chromatographic system is designed in a special way to withstand high system back-pressures.

This instrument is necessary to accommodate the expanding scientific needs of the Helping to End Addiction Long-term (HEAL) and Automated Synthesis Platform for Innovative Research and Execution (ASPIRE) initiatives within NCATS. Under the auspices of these programs and in adherence with the NCATS translational mission of decreasing the time from research discovery to patient treatment, an Automated Chemistry Group was created to investigate and develop an expedient, robust, and flexible automated pipeline for the synthesis of chemical libraries related to biological targets of interest. Due to the potentially large number of samples that can be generated by automated synthesis in a very short amount of time, analytical analysis can easily become a process bottleneck. To meet these high-throughput demands, Analytical Chemistry must employ analysis techniques that have comparable throughput rates while maintaining requisite performance levels. Unfortunately, the current chromatographic analysis capabilities at DPI are limited to traditional High-Performance Liquid Chromatography (HPLC). The experimental runs times when using this instrumentation are on the order of five (5) minutes, which can lead to significant delays when analyzing a 384-well plate looking at reaction optimization. The utilization of a UPLC system provides a significant reduction in run time (1-2 min) while increasing resolution and sensitivity compared to traditional HPLC. Additional advantages include the need for smaller sample amounts and the use of less solvent per experimental run, which is a cost savings over time and increases safety due to minimized waste generation. UPLC-MS is an extremely powerful analytical tool that chemists can use in the identification and quantitation of various chemical entities ranging in size from small molecules to antibodies. Access to the top-of-the-line UPLC-MS instrument, which possesses the highest level of experimental capabilities is an imperative to propel DPI research forward in the successful pursuit of the NCATS mission.

3. Generic Name of Product Ultra-Performance Liquid Chromatography (UPLC) system coupled with a Mass Spectrometry (MS) detector

4. Type of Purchase Description

□ Brand-name or Equal X Brand-name Specific (Item peculiar to one manufacturer) (requires a sole-single source justification)

5. Physical, Functional, and Performance Specifications The system that meets the needs of this requirement must be manufactured by Waters Technologies Corporation and include the following products/quantities:

Mfg. Product # Qty Description 176815023 1 ACQ. UPLC I-Class PLUS Triple w/QDa Sys 176015112 1 ACQ. I-Class PLUS Sys. (CM-A) w/SM-FL-I 176015090 1 ACQUITY UPLC I-Class PDA Detector 700011803 1 ACQ. I-Class PDA Tubing Single Detector 176003208 1 ACQUITY QDa Detector (Performance) 176004266 1 ACQUITY I-Class PLUS QDa Kit 205001275 1 Kit, Ethernet Switch Box w/ Bracket 700009641 1 ESI PROBE ASSEMBLY 250mm 176003244 1 ISM & ACQUITY Splitter Kit 176015027 1 ACQUITY UPLC ELSD w/Nebulizer 700002848 1 ACQUITY UPLC / UV and ELSD Qualification Standards Kit 700002669 1 ACQUITY UPLC / UV Start Up Standards Kit 700002741 1 ACQUITY / Quattro micro or Quattro Premier MS Start Up Solution Kit 176001060 1 ML 4.1 & Standard PC 668000273 1 MONITOR, Lenovo ThinkVision Flat Panel 176015040 1 ACQUITY UPLC SO H/I-Class 205001832 1 Kit, ACQUITY UPLC 4 x 50mm CM-A 186007088 1 Certified Container Kit 741000472 1 QDa SYSTEM INSTALLATION CERT 176001075 1 OpenLynx Open Access v4.1

6. Delivery Date or Period of Performance 8 to 12 weeks ARO

7. Delivery Location National Center for the Advancement of Translational Sciences, 9800 Medical Center Dr., Bldg. A, Rockville, MD 20850 Attention: Sam Michael