PD.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The National Center for Advancing Translational Sciences (NCATS) Division of Preclinical Innovation (DPI) requires one Automated Cell Culture System on a brand name or equal basis. This equipment shall be used to support novel assay development and validation screening.

2. Background Information and Objective:

The Stem Cell Translations Laboratory (SCTL) and the 3D BioPrinting Lab within the Division of PreClinical Innovation (DPI) of the National Center for Advancing Translational Sciences (NCATS) seek to purchase a Sartorius CompacT SelecT Cell Culture System. These labs are in need of a complex automated cell culture system that will consolidate stem cell assay development, assay miniaturization applicability and adaptation to evaluate qHTS feasibility, and validation of promising new assay technologies that had previously been done piecewise by different project teams who divided project workflow between data production and these experimental evaluations. The Stem Cell group will unburden separate project teams from these tasks, while maintaining full system compatibility with the automated robotic qHTS facility, such that validated assay methods and promising assay technologies can be immediately implemented in large scale drug discovery campaigns with confidence that protocols and methods work in the high-density formats and apparatus used by the qHTS facility. This request is required for these Laboratories as part of the overall mission to develop and deploy new technology and assays for the screening of disease targets using stem cells. Given the nature of the work with a vast amount of different stem cell lines, the Stem Cell group requires some automated device that can assist in the production and maintenance of stem cell lines on an industrial scale. The TAP CompacT Cellbase System was designed to fulfill exactly that purpose.

The production of cells in large quantities and in consistent quality is a critical step for the implementation of cell-based high throughput screens. Current cell production for HTS is done manually, which leads to suboptimal batch-to-batch consistency, it is a contamination-prone process, it has limited scalability, and in general, it lacks the needed industrial scale reproducibility. This leads to inconsistencies in the results of the screens, reduced productivity, and increased cost. This is particularly challenging when using stem cell and stem cell-derived cells because of the long duration cultures, numerous media exchange steps to introduce differentiation media, and in general, the high number of steps in the processing protocols.

Automated tissue culture enables cell production with greater reproducibility and productivity.

There is a limited selection of reliable, industry-scale automated tissue culture systems. The SelecT automated cell culture system was developed >5 years ago, has over 40 installations world-wide, and has proven to be able to enable the production of multiple cell-lines and assay-ready plates. The SelecT system can maintain and expand adherent cells in T-175 flasks ensuring that there is no cross contamination even when many cell lines (up to 182) are cultured in parallel; the instrument can be programmed to do media exchanges and so that cells can be harvested, counted and seeded without operator intervention to help optimize screening productivity. It has the capability of plating cells in into 96, 384 and 1536-well plates, or just harvest cells for ready use. Furthermore, the instrument has the capability to measure growth rates so that it can be programmed to harvest cells at previously specified cell confluency. The instrument is also compatible with 10- layer HYPERflasks to increase cell production.

3. Generic Name of Product

Automated Cell Culture System

4. Type of Purchase Description

X Brand-name or Equal

□ Brand-name Specific (Item peculiar to one manufacturer) (requires a sole-single source justification)

5. Physical, Functional, and Performance Specifications

Specifically, the NCATS DPI requires the following, or its equal:

1. One (1) TAP Biosystems CompacT SelecT APM

2. Installation of Requirement 1 at the NCATS facility

For reference, the system that meets the needs of this requirement must include the following Sartorius Part Numbers:

ETO-AC-00008 Core Module ETO-AC-00008 Advanced Plating Module ETO-AC-00008 TAP Extended APM Plate Hotel (10 each) ETO-AC-00008 Compact Startup Kit ETO-AC-00008 Processing of Nunc Triple Flasks ETO-AC-00008 Processing of Corning HyperFlasks ETO-AC-00008 Low Volume Reagent Dispensing ETO-AC-00008 Email Alerts ETO-AC-00008 User Defined Flask Types ETO-AC-00008 Maintenance Spares kit N/A System delivery, installation, trainining, operator spares, service and support

The Cell Culture System must meet the following requirements:

1. The CompacT CellBase must include:

Flask incubator 130 T-75 or T-175 Flasks 6-axis Anthropomorphic Robot Arm Cell Counter Pumps for 10 media lines SelPlan scheduling and capacity planning software

The Advanced Plating Module must include:

Processing area collection pot (600ml) Plate incubator - 140 to 280 plate capacity depending on configuration Plate Bar Code Reader Hotel Bar Code Reader SelFeeder dispenser/aspirator 6, 24, 96, 384 well plate dispensing 24, 96 insert-well plate dispensing 6, 24, 96, 384 well plate media exchange 24, 96 insert-well plate media exchange

We require 10 plate hotels-can be a combination of hotel types:

TAP Extended APM Plate Hotel, 1x 28-slot hotel for 96/384 well plates TAP Extended APM Plate Hotel, 1x 19-slot hotel for 6/24/insert well plates

2. CompacT Start Up Kit

3. Processing of Nunc Triple Flasks

4. Processing of Corning

HYPERFlask

5. Low Volume Reagent

Dispensing

6. E-mail Alerts

7. User Defined Flask

Types

8. Maintenance Spares Kit

Specifically, the NCATS DPI requires the following, or its equal:

One (1) TAP Biosystems CompacT SelecT SC APM Installation of Requirement 1 at the NCATS facility

The brand name or equal system must meet the following requirements:

1. The system must be capable of culturing multiple cell lines with cell line specific protocols.

2. It must be able to operate unattended, enabling more effective use of valuable resources

3. The system must be able to culture delicate or complex cell lines such as stem cells and primary cells.

4. The system must be capable of maintaining stock cell lines for multiple projects.

5. The system must facilitate the output of cells to an external collection vessel allowing multiple flasks to be to be harvested and pooled without restricting the volume of cell suspension that can be collected

6. The system must be equipped with a cooled reagent storage system (6°C / 43°F) to enable low volume dispensing (100 - 1,000μL) for up to 6 reagents such as growth factors and transfection mixes to flasks

7. The system must have the ability to process Corning HYPERFlasks, providing a 10-fold increase in cell production capability

8. The system must be able to send system updates via email.

9. The system must automatically back up valuable information to remote media

10. The system must allow users to generate custom formatted reports

11. The system must be able to generate assay-ready plates for cell-based screening and assay development. Must be capable of processing 6, 24, 96 and 384-well standard plates and also 24 and 96-well insert plates

12. The system must be equipped with a barcode reader on the plating module to track plates during operation.

13. The system must enable direct imaging of cells in flasks, confluence measurement and enhanced QA of cells.

Installation, training and delivery shall be performed by the contractor. Must include operator spares and first year Comprehensive Service and Support contract.

6. Quantity One

7. Delivery Date or Period of Performance Nine to Twelve (9 to 12) Months ARO

8. Delivery Location

National Center for the Advancement of Translational Sciences 9800 Medical Center Drive, Bldg. C, Rockville, MD 20850 Attention: Sam Michael