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Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Statement of Need and Purpose: The purpose of this acquisition is to obtain one (1) Maestro Pro on a brand name only requirement for the Stem Cell Translation Laboratory (SCTL) within the National Center for Advancing Translational Sciences (NCATS) Division of Preclinical Innovation (DPI). This system allows SCTL scientists to measure electrical network behavior in live cells, such as nociceptors and cardiomyocytes derived from induced pluripotent stem cells, at high throughput.

Background Information and Objective: The Stem Cell Translation Laboratory (SCTL) within the National Center for Advancing Translational Sciences (NCATS) Division of Preclinical Innovation (DPI) requires the Maestro Pro to meet SCTL project milestones. The Maestro Pro allows for simultaneous live, label-free recording of electrically active cells. This system will be utilized to characterize neurons; such as nociceptors and cardiomyocytes derived from induced pluripotent stem cells. Characterization of these cells with the Maestro Pro will allow SCTL scientists to gain a better understanding of the behavior of diseased cells, screen for drugs to treat that disease/disorder and examine potential toxic effects of drug candidates.

The Maestro Pro is compatible with the Axion Classic and CytoView Micro Electrode Array plates which the SCTL currently utilizes in experiments, and it is imperative for the continuation of cell characterization. This system is also compatible with the NCATS’ previously purchased Axion Maestro system, but allows for long term, live cell, and label-free recordings due to its fully enclosed cell growth chamber that allows for better CO2 and temperature control. The Maestro Pro is an upgraded system compared to the previous acquisition and will also allow NCATS to run two recording in parallel as more lab members are using the system currently in the lab.

Therefore, this equipment is essential for achieving the SCTL research goals of: 1) establishing detailed quality control (QC) standards to define pluripotency and differentiated cell types; 2) using multi-omics methods to assess molecular and cellular variations/signatures in cellular phenotypes derived from iPSCs; 3) develop standardized methods for producing mature cells from iPSCs that meet QC and reproducibility standards; and 4) discovering, validating and disseminating small molecule reagents to replace expensive recombinant proteins, xenogenic material and undefined media components in cell differentiation protocols.

Generic Name of Product:

1. Microelectrode Array (MEA) Platform

Purchase Description:

1. Maestro Pro (Product # MAESTRO768PRO, Axion Biosystems)

2. Installation and Training, 2 days (Product # INT-OS, Axion Biosystems)

3. Performance Assurance Service Plan with Preventive Maintenance - Maestro Pro (Product # PASPM768PRO, Quantity: 2, Axion Biosystems)

Salient Characteristics:

The Maestro Pro system must include the following essential characteristics:

1. The system’s ability to quickly and easily measure electrical network behavior in live cells at high throughput.

2. The system must be able to detect key parameters of cardiomyocyte activity, including depolarization, propagation of excitability, repolarization, beat timing, and irregular beating (arrhythmia).

3. In addition, the system must include local extracellular action potential assay which allows for recordings of extracellular action potential waveforms which are stable for 10- 20 minutes or more.

4. The system must allow for quantification of action potential morphology and characterization of complex repolarization irregularities.

5. The system must fit in the allotted space within the SCTL laboratory. The dimensions must be H 22.5 CM (8.86”) x W 28.0 CM (11.0”) x D 45.2 CM (17.8”).

The Maestro Pro system must include the following:

• 768-channel MEA system with x12 BioCore v4 chip technology

• Touchscreen real-time interface, stimulation, recording and data acquisition system

• Automated temperature and CO2 control

• Plate usage is logged via an integrated plate barcode scanner for experiment tracking

• Desktop computer, monitor, and AxIS Navigator software for acquisition and analysis

• Compatible Axion BioSystem's 12, 24, 48, and 96-well, MEA plates (SLAS compliant)

• 1 TB storage space

• Electrical stimulation of 768 microelectrodes in all wells simultaneously

• 100-240 VAC power requirements

• 100% CO2 @ 15 PSI gas requirements

• 1-year warranty

• 2 additional years option warranty with Preventive Maintenance

• On-site 2-day installation and training covering hardware, software and applications

Quantity:

One (1) x Maestro Pro system One (1) x Installation and Training, 2 days Two (2) x Performance Assurance Service Plan with Preventive Maintenance - Maestro Pro

Delivery Date: Delivery to be made within 4 weeks of receipt of order by vendor.

Period of Performance: N/A

Option(s):

Two (2) years Performance Assurance Service Plan with Preventive Maintenance - Maestro Pro, additional 1 year in-factory service and preventive maintenance plan including all parts and labor. Includes one in-factory preventive maintenance service.