NICHD-21-214 Questions and Answers.7.27.2021.pdf
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- Procurement of Next Generation DNA Sequencing Federal contract opportunity
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- NICHD-21-214
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NICHD-21-214 Questions & Answers
1. Will the genome that is being sequenced be only human or will there be mouse genome, as well?
Answer: We are working with both human and mouse samples, so, there will be a mix of both.
2. Can you give a little more insight as to how many samples will be processed?
Answer: We have started a new project and we anticipate needing about 100 lanes of sequencing, with a yield of 740M PE reads per lane, with a read length of 150 bp (per read). We won’t be sending all the samples at the same time, we will send maybe 3 samples to be run in 3 lanes per week. Also, we will have a mix of sample types, mouse and human, and chip-seq and whole genome sequencing.
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