SOW.doc

STATEMENT OF WORK

C-Req: ___NFFS7100-16-03314___

PURPOSE:

Increasing CO2 levels in the atmosphere, caused primarily by human activities, are leading to an increase in the dissolved CO2 concentrations in the oceans and a subsequent decrease in the pH of seawater. This decrease in pH, known as ocean acidification is likely to have a substantial effect on marine ecosystems within the next century. It is therefore imperative for the National Oceanic and Atmospheric Administration (NOAA) and the National Marine Fisheries Service (NMFS), in particular, to determine the effects of ocean acidification on fisheries species and the ecosystems on which they depend so that we may be able to adapt our management strategies accordingly.

Our goal is to expand upon current research investigating the effects of ocean acidification on federally managed crab species in Alaska. We will continue to systematically perform laboratory experiments on a range of life-history stages for affected species in Alaska.

TECHNICAL SPECIFICATIONS:

In each of the experiments, water is flow-through sand-filtered seawater at project -specific specified temperatures and a rate of from 1 to 4L/min. The pH in the treatment tanks is adjusted by adding CO2 to the water. pH and temperature is measured daily. Once a week water samples are taken from each treatment, poisoned with mercuric chloride and sent to a laboratory for dissolved inorganic carbon (DIC) and total alkalinity analysis. To assess the environmental conditions in the treatment systems the laboratory will measure alkalinity, pH, and DIC with a high level of precision and accuracy. Approximately 270 water samples will be fixed with mercuric chloride and sent to a chemistry lab for processing. DIC should be measured to within 0.1 umol C/kg and total alkalinity to 0.1 umol/kg. It is expected that the water samples will need to be run through July 2017 with the possibility for three more years of samples dependent on annual funding.

Tasks:

A.) Measure Alkalinity (total alkalinity to 0.1 umol/kg B.) Measure PH

C.) Measure Dissolved Inorganic Carbon to within 0.1 umol C/kg

D.) The Alkalinity and DIC measurements need to be made with a VIndta 3s system to be compatible to that used by the Kodiak Lab.

DELIVERABLE SCHEDULE:

08/01/2016 – 07/31/2017 Option Year 1 08/01/2017 – 07/31/2018 Option Year 2 08/01/2018 – 07/31/2019

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