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Naval Research Laboratory
EQUIPMENT PROCUREMENT SPECIFICATIONS DOCUMENT
ITEM: RAMAN IMAGING MICROSCOPE WITH SCANNING LINE FOCUS
The Naval Research Laboratory requires a commercially available, research grade imaging Raman microscope, with line focus scanning, to be installed in an optical laboratory.
It will be housed on an existing 4 foot (48 inch) wide optical bench.
It will be used frequently for a long periods of time, for a broad variety of applications, so its compatibility with our existing instruments, as well as its durability, performance, flexibility and speed, will be critical for our applications.
The compatibility with our existing 532nm laser (Coherent Verdi V6) and existing Electron Multiplied CCD detector (Andor Newton DU970P-BV) will be critical to using the microscope for optimal Raman spectroscopic and chemical imaging. Its expandability will be vital to our ability to adapt the system for future applications.
| REQUIREMENT |
| SPECIFICATION |
| 1 |
| Variable spot size |
| Must be able to continuously vary the laser focal spot size at the sample from the diffraction limit (~1 micron) up to 300 microns |
| 2 |
| Line Focus |
| Motorized cylindrical lens for computer controlled focus of the laser spot into the shape of a line for mapping with increased throughput and decreased laser power density. |
| 3 |
| Real-time autofocus |
| Real time automated dynamic focusing for both Raman data acquisition (all lasers) and white light viewing modes (up to 3 laser sources). This enables the microscope to adjust the Z stage to maintain optimal focus on the laser illuminated spot even if the height of the sample changes during the measurement, such as during melting. |
| 4 |
| Arbitrary spectral coverage |
| Facility for measurement of high resolution spectra with wider (arbitrary) wavelength range than can be accommodated on a single CCD exposure, without any stitching of spectra together. Spectral resolution should be continuously variable via CCD binning control. |
| 5 |
| Scripting |
| Ability for the user to call a function (like a “macro”) to implement routinely repeated measurement or processing steps within an experiment |
| 6 |
| Remote access |
| Ability for the user to remotely control the microscope and spectrometer through an internet connection for unattended operation and data collection |
| 7 |
| Auto alignment |
| Automatic alignment and optimization of input laser power |
| 8 |
| Auto switch of laser line |
| Automatically switch incoming laser wavelengths and automatically align laser through beam expander motorized pinhole assembly |
| 9 |
| Raman self validation |
| Self validation using internal reference sample (such as silicon) |
| 10 |
| Built in intensity and wavelength calibration |
| Built in calibration using internal white light (for intensity) and neon (for wavelength) sources |
| 11 |
| Simultaneous laser and white light sample illumination and observation using built-in camera |
| Must be able to simultaneously illuminate the sample with both white light for imaging and the laser spot for registry. Must have built-in camera to record sample images |
| 12 |
| High efficiency spectrograph |
| Must be at least 30 % efficient to allow fast Raman imaging |
| 13 |
| Continuously adjustable confocality |
| Continuous adjustment of the motorized slits to enable an arbitrary effective confocal dimension. |
| 14 |
| CCD Array response |
| CCD array with non-fringing spectral response from 400nm to 1050nm. |
| 15 |
| CCD readout speed |
| CCD should be able to read out up to 1600 times per second |
| 16 |
| objectives |
| Microscope must have 5X (0.12NA), 20X (0.12NA), 50X (0.5NA) (working distance 8.2 mm) and 100X (0.85NA) objectives |
| 17 |
| binocular |
| Microscope binocular with 10X eyepieces |
| 18 |
| 633 nm laser |
| 17mW 633nm laser and associated optics |
| 19 |
| 633 nm edge filter |
| Edge filter rejecting the Rayleigh line to 50 cm-1 from 633nm (ripple-free throughput) |
| 20 |
| 532 nm edge filter |
| Edge filter rejecting the Rayleigh line to 50 cm-1 from 532nm (ripple-free throughput) |
| 21 |
| Port for 532nm external laser |
| Must have modular port to introduce an external 532nm laser source (existing Coherent Verdi V6 laser) |
| 22 |
| gratings |
| 600 lines/mm and 1800 lines/mm (automatically switchable within the software) |
| 23 |
| Spectral resolution |
| Raman spectral resolution of 1.5 cm-1 or better |
| 24 |
| Polarization rotation |
| Motorized ½ wave plate at laser source, motorized ¼ and ½ waveplates for the collected Raman and Rayleigh scattered light |
| 25 |
| software |
| User friendly software to control the system, take unattended data cubes, process them and generate presentation quality images. |
| 26 |
| XYZ controlled position mapping |
| Able to generate Raman images along a 1D line (X,Y), 1D depth (Z), 2D area mapping (XY), 2D depth slices (XZ, YZ) |
| 27 |
| Fast imaging with parallel data collection |
| Produce Raman chemical images up to 100× faster by using line focus illumination, with parallel data collection, enables higher laser powers to be employed. Includes automated surface tracking to maintain sample focus. |
| 28 |
| Software processing for sub laser spot high spatial resolution |
| Reveal sample features significantly smaller than the size of the laser spot by taking advantage of the 100 nm step size of the encoded stage. Includes automated surface tracking to maintain sample focus. |
| 29 |
| Integration with existing EMCCD detector |
| Must have a port at the exit of the spectrometer to house our existing EMCCD detector. This is to accommodate and to electronically and software interface to an existing Andor Newton DU970P-BV EMCCD detector. |