Microscope Statement of Need.pdf

Statement of Need

Upright Compound Microscope Camera System

Purpose: Acquisition of a new upright compound microscope and camera system (microscope, objective lenses, optical filters).

Background: The National Centers for Coastal Ocean Science, Beaufort Laboratory requires a new upright microscope and camera system to meet the microscopy goals for ongoing HAB forecasting projects.

Specifications: The camera system shall include an upright compound microscope, high resolution objective lenses, and a digital camera system in accordance with the following specifications:

• Microscope Upright compound microscope shall include 360 degree rotating trinocular head with light transmittance settings allowing 0%/100%, 100%/0%, and 80%/20% of the light through the eye pieces/camera port.

Shall include two locking 10x 20mm focusable eyepieces.

Shall include nosepiece having ports for at least 6 objective lenses Shall include mechanical stage with adjustable tension controls and high strength glass insert.

Shall include LED illumination with variable intensity control.

Shall include Infinity Optics with anti-mold coating.

Shall include epifluorescence attachment holding at least 6 filter positions, and with adapters/connectors to fit to a Cool LED pE-300 white Illumination System currently owned by the government.

Shall include high resolution condenser with settings for all objective lenses (see below).

Shall include Optical filter holder capacity of at least two (2) filter blocks.

• Objective Lenses: Shall include the following objective lenses optimized specifically for fluorescence microscopy.

4x lens, NA at least 0.13 10x lens, NA at least 0.30 20x lens, NA at least 0.50 40x lens, NA at least 0.75 60x lens, NA at least 0.85 100x Oil Immersion lens, NA at least 1.3

• Epifluorescence Optical Filter sets: Shall include filter cubes holding optical filters compatible with the microscope (25mm diameter excitation and emission filters, dichroic mirror). Filter cubes shall be required for the following fluorescent dyes:

o GFP/FITC filter cube: Excitation wavelength ~480 nm, Emission ~535 nm o DAPI filter cube: Excitation wavelength ~395 nm, Emission ~460 nm o TRITC filter cube: Excitation wavelength ~545 nm, Emission ~605 nm o Texas red filter cube: Excitation wavelength ~560 nm, Emission ~630 nm

Delivery Schedule: All components shall be delivered to the Beaufort Laboratory no later than 30 September, 2022.

Government Point of Contact:

Steve Kibler NOAA Beaufort Lab 101 Pivers Island Road Beaufort, NC 28516 Ph: (252) 666-7476 Steve.Kibler@noaa.gov