Microscope - PRE SOLICITATION NOTICE.docx

PRE SOLICITATION NOTICE

Background VA Pittsburgh Healthcare System (VAPHS) is in need of an Olympus FV4000 Confocal Microscope System. This is to replace the existing FV1000 system which is an older Olympus model which is now antiquated technology and at end of life. This model will be used for all Researchers that come to VA Pittsburgh and use this microscope to conduct scientific studies at the University Drive campus, Research Building.

Deliverables/Requirements Olympus FV4000 Confocal Microscope System to include:

· FV40-HSU-P: Package of a hybrid scanning unit and a power supply unit and a 2ch broadband spectral detector unit

· FV30-STIMP: ADAPTER TO CONNECT SCANNER AND IX83 SCOPE FRAME

· FV40-RSD: 2ch red-shifted spectral detector unit by silicon photomultiplier including secondary dichroic mirror turret.

· FV40-LETD: Transmitted detector unit for FV4000 used with IX3-LHLEDC. 5 1

· LASERS

· FV40-MCOMB: Main laser combiner including AOTF and 4 lasers(VBYR). Does not include lasers.

· FV31-LD405: LASER DIODE 405NM,CW 50MW 8 1

· FV31-LD488: LASER DIODE 488NM,CW 20MW 9 1

· FV31-LD56: LASER DIODE 561NM,CW 20MW 10 1

· FV31-LD640: LASER DIODE 640NM,CW 40MW 11 1

· FV31-LDPSU: FV3000 6 CH laser diode power supply 12 1

· COMPUTER AND SOFTWARE

· F30PCA19S: FV Workstation HP Z4 G5 Tower. Intel XeonW-2223 3.6GHz, 512GB PCIe NVMe SSD + 256GB PCIe NVMe + 2TB HDD, 64GB RAM (4x16GB) DDR4, Nvidia RTX A4000 16GB Graphics Card. Includes IEEE1394a cable (9pin to 6pin) for BX3-CBH or IX3-CBH.

· IA-HDD-4TB: Seagate FireCuda HDD 4TB Internal Hard Drive

· 7-DP21DKT: General IEEE1394a cable (9pin to 6pin) for BX3 CBH, IX3-CBH, or DP21 with Desktop PC. 2.7m

· FV40-PCIB: PCI-e interface board for data transfer for confocal laser scanning microscope. Corresponded to WEEE and RoHS Regulations.

· D-U4323QE: Dell UltraSharp 43" 4K USB-C Hub Monitor 18 1

· UYCP-11: US Style 3-prong Power Cord 19 5

· OCD-11760: Line Cond 1200W AVR Surge 120V 10A 60Hz, 7 Cord 20 1

· FV40-SWPAD: Advance Package software for FV4000. This software includes FV40S-SW main software, motorized stage control license, well navigator license and sequence manager license including License certificate sheet

· CS-S-DE-VF: cellSens TruSight 3D Deconvolution Solution

· CS-DI-DT-V4.2: Cellsens Dimension Desktop Version 4.2

· MICROSCOPE FRAME

· IX83P2ZF-1-3: IX83 Inverted, Motorized Microscope Frame with 2 Decks

· IX3FP: FIXING PLATE ON OPTICAL BENCH

· U-I120: IX3-CBH; CONTROL BOX FOR IX83

· U-R944: U-MCZ-1-2; FOCUS CONTROL MODULE FOR BX63/IX83

· U-IFCBL200-1-2: Interface Cable for IX3/BX3/STM7 - 200CM

· FV30-RFACA; MOTORIZED FLUORESCENT MIRROR TURRET

· U-B30015: U-IFCBL15; CONNECTION CABLE FOR IX3/BX3 - 15CM

· IX3-ILL-1-2: IX3 Transmitted Light Pillar

· F12IX3TFC: FV12-IXCOV;IX3 TRANSMISSION FILTER COVER

· 45FR-1-2: 45MM Diffusion Filter

· IX3-LHLEDC-1-3: LED Light Source for IX3-ILL

· 6-U2701: IX3-LWUCDA-1-2; Motorized Long WD IX3 DIC/Phase Condenser, NA 0.55, WD 27MM

· U-B30100: U-IFCBL100; Connection Cable for IX3/BX3, 100CM

· FV31-ILLSW: Laser Interlock Safety Unit for IX3-ILL

· FV30-ILSW: LASER INTERLOCK SWITCH FOR IX83 STAND

· U-TBI90-1-2: Tilting Binocular Head for GX/IX, 35-85 degrees

· WHN10X-1-8: Eyepiece for BX/IX, 10x w/ Φ=24mm t=1.5mm Retical Shelf, FN22

· WHN10X-H-1-8: Eyepiece for BX/IX, 10x Focusing w/ Φ=24mm t=1.5mm Retical Shelf, FN22

· OBJECTIVES

· UPLXAPO20X: UPLXAPO20X OBJECTIVE, OFN26.5, NA0.80, WD0.6mm

· UPLXAPO40XO: UPLXAPO40xO OIL OBJECTIVE, OFN26.5, NA1.40, WD0.13mm

· UPLXAPO60XO: UPLXAPO60X OIL OBJECTIVE, OFN26.5, NA1.42, WD0.15mm

· UPLXAPO100XO $0.00 $0.00 UPLXAPO100X OIL OBJECTIVE, OFN26.5, NA1.45, WD0.13mm

· U2-CD410: IX2-DIC100; Nomarski Prism for 100X Objective, 38MM

· U-M636I: IX3-FDICT; Analyzer Cube for DIC W/IX3-RFACS

· IMMOIL-F30CC1-3: Immersion Oil 30cc Bottle $0.00 $0.00

· VISUAL FILTERS AND LIGHT SOURCE

· OCL-PE300-WL-SB-B: pE-300White LLG Single Band Filter system, with adapter and power cord

· OCT-49000BX3: ET-DAPI Filter Set 350/50X, BS400, 460/50M w/BX3 cube

· OCT-49002BX3: ET-EGFP/FITC/CY2 Filter Set 470/40X, BS495, 525/50M w/BX3 cube

· OCT-49004BX3: ET-CY3/TRITC Filter Set 545/25X, BS565, 605/70M w/BX3 cube

· STAGING

· MZ-IX3SCAN-P: 48-24-581-0000; Scan plus IM 120x80 motorized stage for IX3. Linear encoding

· MZ-DTC3: TANGO 3 DESKTOP CONTROLLER

· MZ-CABLE2X: 00-76-202-0808;2 AXIS, 2M CABLES

· MZ-JYSTK2: 00-76-200-0820;2 AXIS JOYSTICK

· MZ-INS-8020: 00-29-411-8020; Stage insert for multi well plate (K-Style 160x110)

· MZ-INS-8050: 00-29-411-8050; Stage insert for Petri dish, Ø 36 mm (K-Style 160x110)

· REQUIREMENTS

· FV31-SPCOV: On Stage Specimen Cover

· FV40-SAFI3BE: Class 3B laser safety label in English for FV4000 inverted microscope configuration

· This system will reuse Olympus parts from the previous Olympus confocal system, including:

· 10x UPlanSApo objective

· 20x LWD Fluorite Objective

· Anti-vibration table 10/20/40/60x DIC prisms U-DICT insert Wrightline furniture

· The Olympus FV4000 microscope that uses a silicon-based SilVIR detector with a fully modular platform that allows reconfiguration of the system as needed.

· Ability to capture both dim or right fluorescence areas in one image without saturation or loss of information.

· A SilVIR detector that doesn’t need to make any voltage and offset adjustments.

· Reproducible Image Data Between Users and Systems

· Precise Dynamics of Live Cells with Less Damage using time-lapse imaging via the 685 nm, 730 nm, and 785 nm lasers with a dedicated TruFocus Red Z-drift compensator to maintain the focus position.

· TruAI Noise Reduction and Image Segmentation

· Asilicon photomultiplier (SiPM) with fast signal processing technology.

· Flexible Macro to Micro Imaging

· High-Resolution 3D Images in Thick Samples

· Clear Images at Depth with silicone immersion objectives

· A spectral system comprised of volume phase hologram (VPH) grating and slit and can detect a 400 nm to 900 nm wavelength range with a minimum step of 1 nm.

Technical Specs

Period of Performance

· Delivery and installation 8-12 weeks after the award.

DELIVERY

1. Delivery location will be the Research Office Building, 4071-4089 University Dr. C, Pittsburgh, PA 15219 Building 30 Wet Labs. Please coordinate with the POC for delivery after contract award.

REPAIR AND MAINTENANCE

1. The vendor/distributor and/or the manufacturer must provide all necessary procedure manuals, troubleshooting manuals and operator manuals.

On site Installation

1. The vendor will assemble the unit on-site to be scheduled by VAPHS. The vendor will unload, stage, assemble, test, clean up and remove the debris. They will be on site for no more than 8 hours.

2. The COR is responsible for ensuring that all Contractors comply with physical security policies. A compliant ID badge must be worn by Contractors at all times while on VA premises.

3. Contractors may not have access to the VA network or any VA sensitive information under this contract.

4. All Contractors must receive Privacy training annually using one of the following methods:

a. Complete “VA Privacy Training for Personnel without Access to VA Computer Systems or Direct Access to or Use to VA Sensitive Information” training by using VA’s TMS system (https://www.tms.va.gov/SecureAuth35/). Contractors may use the TMS Managed Self Enrollment method to complete the training in TMS.

5. Complete the hard copy version of “VA Privacy Training for Personnel without Access to VA Computer Systems or Direct Access to or Use to VA Sensitive Information.” Signed training documents must be submitted to the COR.

6. Training must be completed prior to the performance of the contract and annually thereafter. Proof of training completion must be verified and tracked by the COR.

Smoking All VA Pittsburgh property is smoke-free. Contractor personnel will comply with VA Pittsburgh smoking policies. Violations will result in a warning and possible citation.

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