Keyence All in One Fluorescence Microscope Unit Performance Work Statement PWS - Specifications.docx

UNITED STATES DEPARTMENT OF AGRICULTURE

AGRICULTURAL RESEARCH SERVICE

CROP GENETICS RESEARCH UNIT

PERFORMANCE WORK STATEMENT

SPECIFICATIONS

Keyence BZ X810 All-in-One Fluorescence Microscope

1. Introduction: A Project Plan objective of the CGRU requires working with cooperators at North Carolina State University (NCSU) and use new fluorescent imagery to study cotton fiber development. The technique requires a number of different magnification objectives, sampling filters and software. As the project progresses, additional microscopy capabilities will be needed and can be obtained by adding accessories to the existing Keyence unit instead of buying a new microscope. NCSU developed the technique using the Keyence all-in-one unit and we need to have the same unit to be able to conduct our part of the research and produce images and data that are comparable to previous and future results obtained by NCSU.

2. Scope: The Contractor shall provide the BZX Fluorescence Microscope unit, BZX Microscope Head, BZ Support Package, BZX Hardware Module, BZX Multi-Stack Module, BZX Software, BZX Analyzer Software and BZ Objective Lens 2X, 10X, 20X.

3. Technical Requirements: Said device must meet or exceed the following requirements.

· Fully enclosed inverted type fluorescence phase-contrast microscope with fully digital output

· Objective lens switching: Six-mount electronic revolver

· Objectives: High quality objectives, 2 -20x magnification with capability to upgrade to 100X.

· Electronic XY stage: 40 x 40 mm stroke, 1μm pitch minimum

· Electronic Z stage: 8mm stroke, 0.1 μm pitch minimum

· Electronic Filter turret: Four filters mounted, automatic excitation shutdown during filter replacement

· Fluorescent Incident Illumination: Rod fiber optical system

· Fluorescence dimming mechanism

· Ultra high pressure mercury lamp: 2000 hour average lifetime

· Black space: the stage is fully contained in a built-in darkroom

· Image pick-up element: 2/3 inch, 1.5 million pixel monochrome CCD

· Output signal, gradations: 12-bit monochrome, 8-bit monochrome, and 8-bit RGB

· Binning: On chip binning

· Number of pixels in recorded image: 4,080 x 3,072 max (12.5 million pixels, high quality interpolation)

· CCD cooling mechanism: Peltier cooling

· Electronic shutter: 1/12,000 sec to 60 seconds

· Electronic revolver control driver software (controlled via mouse)

· Electronic stage control driver software (controlled via mouse)

· Moving image recording software enabled

· Multi-color capturing software, auto overlay

· Multipoint image capturing software

· Image stitching software: 1,200 image maximum stitch

· Z-stacking software

· Automatic cell counting software

· Measurement software and Analysis software

· PC interface IEEE1394a

· Temporary Replacement Units available

· Complete training sessions for each group

4. Point of Contact: Jodi Scheffler, 662-686-5219, jodi.scheffler@ars.usda

5. Period of Performance: one year

6. Government Furnished:

7. Performance Requirement Measures: National direct support coverage at least M-F 7:00 am – 5:00 pm EST, Application support and on-site consulting with advanced notice, temporary replacement units available when repairs needed.