Metaphase_Finder_System_-_Specifications.pdf

Required Specifications:

Offerors responding to this RFQ, must submit a quote for a metaphase finder system meeting the following required specifications.

REQUIRED SPECIFICATION JUSTIFICATION FOR SPECIFICATION

Automated (x, y-,z-axis) High-Grade Research Microscope:

equipped with both bright-field and fluorescence capability;

motorized six position turret, nosepiece, and stand; 2-position trinocular tube; oculars 10x/23; objectives:

PA10x/0.45, PA63x/1.4 oil; 5 color ET filter set (DAPI, Aqua, FITC, Orange, Texas Red) mounts -Scanning platform system consisting of: Desktop PC with Intel Quad Core (i7) processor or equivalent; AMD Radeon 2GB Graphics or better; RAM-16 GB, dual 1TB HDDs - RAID arrayed; USB and IEEE ports; 8X DVS+/-RW Drive; 24" LCD Monitor, monitor calibration; USB mouse (wired); Keyboard with CAC reader; 2 port 2b 1a low profile 1394 PCI Express FireWire card; PC system to be provided to USU to install Windows 7 OS with DICAP compliant and lockdown security software to be installed on USU LAN. Megapixel CCD camera, motorized stage (16 slides) with Interface card (16 slides), calibration slide; Archiving hardware and software.

A single multi-functional (i.e., bright-field, fluorescence) research grade microscope to suppport on-going biodosimetry research activities at AFRRI is required.

Scanning platform system consisting of: Desktop PC with Intel Quad Core (i7) processor or equivalent; AMD Radeon 2GB Graphics or better; RAM-16 GB, dual 1TB HDDs - RAID arrayed; USB and IEEE ports; 8X DVS+/-RW Drive; 24" LCD Monitor, monitor calibration; USB mouse (wired); Keyboard with CAC reader; 2 port 2b 1a low profile 1394 PCI Express FireWire card; PC system to be provided to USU to install Windows 7 OS with DICAP compliant and lockdown security software to be installed on USU LAN. Megapixel CCD camera, motorized stage (5-8 slides) with Interface card , calibration slide; Archiving hardware and software.

Typical studies involve cell samples on multiple slides. A stage to hold multiple slides to permit automated search, identification, and scoring of radiaton-induced chromosomal aberrations is required.

Service maintenance agreement (hardware and software) - 2nd year

Service maintenance agreement are required to support on-going research activities.

Service maintenance agreement (hardware and software) - 3rd year

Service maintenance agreement are required to support on-going research activities.

Desired Specifications:

The following specifications are desired and not required. Offerors responding to this requirement are not required to quote a metaphase finder system meeting all the desired specifications listed below; however quotes meeting or exceeding more of the desired specifications, may be considered more desirable by the Government.

DESIRED SPECIFICATIONS

Karyotyping software: tools to permit orienting chromosomes, separting overlapping and touching chromosome, auto-sorting chromosomes by size and shape, etc.

Fluorescence analysis: ability to use multiple (12) color probes and retain automated capture and analysis capability.

Patient data analysis: software tools to permit multi-analysis, report generation, statistical analysis, and data archiving.

Multi-user stations: ability to connect to a Local Area Network (LAN) so that multiple users can operate metaphase finding system and associated software.

Validated software to support radiation biodosimetry assays including automated scoring of dicentric chromosome aberrations in metaphase spreads with Geisma counter-staining with bright field microscopy, binucleated cells with micronuclei using cytokinesis-blocked micronuclei assay using lymphocytes, FISH translocatons in metaphase spreads using DAPI counter-staining, and gammaH2AX foci using fluorescence microscopy.

Ability to expand for higher throughput involving use of multiple trays holding multiple slides.

The selected contractor will also be required to provide installation and training. No Government furnished equipment or supplies will be provided to the selected contractor for installation or training. Installation and training shall be coordinated with the technical end-user, who will be identified in the purchase order, and be conducted during normal business hours (Monday – Friday: 8:00 AM local time – 4:00 PM local time, excluding Federal Holidays).