Sources_Sought_Specifications.docx

DNA Sequencer and additional system components for Next-Generation Sequencing System

· Multiple flow cell and reagent configurations enable sequencing of 20–120 Gb per run.

· Up to 400 M single read clusters (800 M paired-end reads) passing filter in the High Output configuration enable up to 120 Gb per run.

· Up to 120 Gb of output allows sequencing of a 30× human genome in a single run.

· Up to 120 Gb of high-quality filtered bases in 29 hours on the sequencer (2 × 150 bp reads) or up to ~100 Gb per day.

· No need for emulsion PCR—amplification is performed directly on the instrument.

· Fully automated onboard cluster generation enables prepped libraries to be loaded directly onto the instrument.

· Single instrument performs clonal amplification, sequencing, paired-end turn and primary data analysis (e.g. base calling).

· Scalability from 20–120 Gb in a single run to support a broad range of applications and study sizes

· Desk-top instrument: W×D×H: 58.5 cm × 53.4 cm × 63.5 cm (23.0 in × 21.0 in × 25 in).

· Weight: 83 kg (183 lbs).

· 12 light-emitting diodes at 520 nm, 650 nm.

· Instrument footprint requires less than two square feet of benchtop space.

· Alignment, variant calling, and reporting are supported in BaseSpace.

· Resulting DNA clusters are sequenced using ‘sequencing by synthesis’ method with reversible terminator chemistry.

· Integrated cluster generation provides automated clonal amplification of single molecules randomly distributed on a glass surface.

· Accurate sequencing of homopolymers.

· Real-time analysis processing automatically produces image intensities and quality-scored base calls directly on the instrument computer.

· Sequencing reactions are performed on the surfaces of a multichannel flow cell.

· A single operator can amplify up to 24 samples on a single channel in ~2 hours using a single instrument.

· Two flow cell formats and multiple reagent configurations enable data output from 20–120 Gb in a single run.

· A simple workflow and quick run time to enable the fastest desktop sequencing of exomes, transcriptomes, and whole genomes.

· Massively parallel sequencing technology for genetic analysis and functional genomics.

· Suite of dedicated flow cell and reagent configurations to generate up to 400 million clusters passing filter (up to 120 Gb).

· The system includes an option to integrate with BaseSpace genomic computing environment to store, analyze, and share genomic data, available as either a cloud or an onsite deployment (i.e. BaseSpace Onsite system).

• Number of reads per run:

· Up to 400 million reads per run (e.g. clusters passing filter).

• Throughput per run:

· 100–120 Gb data per 2 × 150 bp run using High Output flow cell and reagents

· Sequencing flexibility

· Set-up options include single-read or paired-end runs

· Flow cell options (e.g. Mid or High Output flow cells) can be used to select data output levels

· Read-length is fully adjustable up to 300 base pairs

Additional System Components

· Prepares libraries for next-generation sequencing on Illumina sequencers. Simple, easy-to-use solution that eliminates most of the manual workflow steps and delivers reproducible, ready-to-sequence libraries.

· Based on digital microfluidics technology, operates using cartridges that contain samples and reagents. All biochemistry operations take place at micro-scale within the consumable in a highly controlled environment.

· Processes 16 samples per run

· Prepares libraries from both DNA and RNA

· Quantifies and normalizes libraries

· Can work with input amounts as low as 10ng

· Generate libraries that can be used on any Illumina sequencer

· System price below $50,000 USD Training Requirements The vendor shall also provide on-site training.