D_Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The core work for the iNDI program is cell culture management and protein isolation for mass spectrometry, western analysis and RNA and DNA sequencing analysis. This is a very critical aspect of the program to support its analysis applications to study the effect of neurodegeneration-related mutations on critical cell biological processes on a biochemistry level. This magnetic particle processor will be used to create high through-put substrates to help identify converging mechanisms of disease across hundreds of isogenic iPSC-derived CNS cell types. The iPSC neurodegenerative disease initiative (iNDI) requires a high content automated system to achieve its goals.

2. Background Information and Objective: The instrument with its unique automation capabilities and design, allows for moving of magnetic beads instead of moving liquids using highly magnetized probes, which leads to cleaner extractions of protein, DNA and RNA. It also ensures consistent results with every run. This unit enables more applications than any other sample preparation instrument, from 6 to 96 samples. This instrument allows for consistent, with very low risk of contamination or human error in preparing samples for mass spectrometry needed for analysis of different cell types.

3. Generic Name of Product: Magnetic Particle Processor

4. Purchase Description:

Item description Part Number Quantity

Kingfisher APEX, 96 DW Head 5400930 1.00 Smartstart KF APEX TRN46245 1.00

Shipping and Handling N/A 1.00

5. Salient characteristics All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Vendor must be able to provide these specifications:

System must provide for an automated magnetic particle processer for the separation, purification and extraction of biological samples, including cells, organelles, proteins, peptides and nucleic acids.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

System must provide magnetic probes which are able to process superparamagnetic particles or magnetic beads which are moved rather than having to move the liquids.

Purification and processing steps are performed in 96-well deep well plates, 96-well plates, 96-well PCR plates, or 24-well deep well plates with dimensions that match the configuration of tip combs that cover the magnetic rods.

System must provide protocols which are selected from a GUI display using a tabbed format navigated via buttons on the instrument keypad.

System must include magnetic beads which are mixed with target nucleic acids, proteins or cells, which bind to the specific coating of the particles or to the particles themselves.

Particles are collected onto polypropylene tip combs that cover the magnetic rods and are dipped into various reagents in deep well plates (96- and 24-well), standard plates or PCR plates for washing and incubation to remove contaminants.

The system processor must eliminate hands-on processing such as ethanol precipitation, phenol/chloroform extraction, centrifugation or column purification thereby freeing staff from the continuous physical strain of manual aspirating, pipetting or decanting of liquids to perform more productive tasks and maintaining health and safety of scientific staff.

The automated processor must provide for optimal reproducibility by eliminating potential inaccuracies introduced by manual methods which is vital and crucial to the mission of the INDI initiative.

The magnetic automated processor must provide for an open platform capable of using any manufacturer’s magnetic particle-based kits and associated protocols and is not limited to running only one kit supplier’s chemistry systems, encouraging protocol development and which can include cost savings for the end user.

6. Quantity:

One (1) Kingfisher APEX 96 DW Head, Part number: 5400930 One (1) Smartstart KF Apex, Part number: TRN46245 Shipping and Handling

7. Delivery Date: Delivery date of 6 months after receipt of completed purchase order

8. Period of Performance: N/A

9. Option(s): Not applicable