D_Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The purpose of this request is to purchase IsoSpark Duo from Isoplexis.

2. Background Information and Objective: The central hypothesis of this project is that immune dysfunction contributes directly to chronic inflammation and inflammation-associated diseases in the elderly. To understand the basis for immune impairment that accompanies aging, we propose to identify age-associated alterations in human innate and adaptive immune cell populations. Towards this goal, we will carry out cross-sectional analysis of proteomes at single cell levels from various immune cell subsets obtained from peripheral blood of 50 participants. Our major objectives are to identify age-associated changes in these parameters in each cell type and to determine how characteristics in a specific cell type change depending on the cellular milieu. Isoplexis technology will provide significant insigts on all the aspect of of hypothesis.

3. Generic Name of Product: Isoplexis IsoSpark Duo

4. Purchase Description:

1- IsoSpark Duo; Product Code # ISOSPARK-1001-1 2- Instalation and training; Product Code # SVCISL-INST-1

5. Salient characteristics:

1- IsoSpark Duo is cutting edge single cell proteome analysis technology.

2- Single instrument platform is capable of performing single-cell proteome, single-cell intracellular signaling, and single-cell metabolomics.

3- Platform is capable of perofming Secretome and fluids (serum/plasma) proteomics.

4- Ready-to-use reagents and Chips.

6. Quantity:

1- IsoSpark Duo: 1 2- Instalation and training: 1

7. Delivery Date: within 30 days from placing the order.

Delivery shall be made to

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

Mary Kaileh Building: BRC, Room: 6C211A National Institute of Aging 215 Bayview Blvd Baltimore, MD 21224