D_Product Description.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The National Institute on Aging LMBI projects elucidate immune cell function in aging and aging-associated diseases. For this purpose, LMBI members process an enormous number of human and mouse samples almost daily. The nature of the modeling studies require handling of samples within the shortest time possible, before the cells acquire non-specific functional changes. Currently, members of the lab end up working up to 18h/day, to finish modeling studies. Exhaustion can lead to mistakes that could jeopardize the results. At least a quarter of this time could be cut with the addition of a high speed automated cell counter.

2. Background Information and Objective: The purpose of this acquisition is to procure one (1) MX-AOPI Cellaca MX High Speed Cell Counter from Perkin Elmer. It enables high speed accurate counting of primary cells.

3. Generic Name of Product: Automated cell counter

4. Purchase Description: MX-AOPI Cellaca MX High Speed Cell Counter equiped with FOMs for AOPI analysis. Brightfield imaging instrument, Fluorescence Optical Platform, Green and Red Channels, Computer controller, USB cable, power supply. Includes a sleeve of 10 counting plates and a sample vial of AO/PI viability stain.

5. Salient characteristics

• Batch samples – run up to 24 samples at a time

• Small sample volume – only 25 µl of cell sample required

• Autofocus – fast autofocus prior to analysis

• Cell based assays – data output at a single cell level enables you to do cell-based assays such as apoptosis, mitochondrial membrane potential, protein expression (including GFP and RFP), and reactive oxygen species (ROS)

• Time course reporting – effortlessly create growth curves with multiple samples at several time points using the customization tools

• Automatic calculation – calculate various data points such as doubling time for individually labeled samples with an easy-to-use interface

• Multiple configurations – choose to add up to five fluorescent filters

• Automation ready – robotic integration ability with optional API

• Customizable reports – add graphs, images, charts, and tables

6. Quantity: 1 item

7. Delivery Date: 30 days after receipt of order

8. Period of Performance: The Contractor shall provide a one (1) year warranty on all equipment from date of installation. All products shall be warranted to be free from material defects in workmanship or material under normal use and service for the warranty period. The Contractor shall provide a certified original equipment manufacturer service technician to perform all repairs during the warranty period.