D-CCS_Attach1_Purchase_Description_BNE.pdf

Purchase Description

Statement of Need and Purpose:

The purpose is to procure an infrared laser-based imaging system for the Therapeutics Development Branch.

Background Information and Objective:

The Therapeutic Development Branch (TBD) within the National Center for Advancing Translational Sciences (NCATS) requires a scanning imager capable of high-throughput detection of samples to meet the needs of the ongoing, multiple collaborative Cooperative Research and Development Agreement (CRADA) and Therapeutics for Rare and Neglected Diseases (TRND) projects.

A new Infrared Imaging System will support the mission of TDB in several ways.

Accurate identification of molecular mechanisms, cellular targets and pathways are important for the discovery of effective therapeutic options. As well, phenotypic screening is a valuable tool in the drug discovery process. TDB employs these methods to screen for potential therapeutic options for rare diseases.

A device capable of imaging different mediums such as flat gels and membranes, cells in culture plates, and tissue sections will be highly advantageous in terms of time, resources, and efficiency. In addition, quick imaging and quantification of samples (such as IN/ON-cell imaging and RNAi analysis) will be an asset to optimize and validate screening assays. High quality, reproducible, unaltered publication ready images are essential for data transparency and accurate conclusions. A compatible software designed to quantify the images with validated normalization techniques is also needed.

For these purposes, an imager capable of high throughput scanning with high sensitivity (i.e. high signal to noise ratio) with fast acquisition times is required. An infrared imager will be ideal to visualize the targets of interest with minimal background autofluorescence, which is often observed in non-laser-based imaging systems. As well, by enabling multiplexing of samples, better accuracy can be achieved while minimizing steps.

Generic Name of Product:

Infrared imaging system

Purchase Description:

Brand-name or equal products to the following:

(1) Trade name: Infrared imaging system

(2) Product’s brand name: Odyssey DLx - Data Integrity Bundle and software

(3) Manufacturer's name: LI-COR

(4) Product number Odyssey DLx - Data Integrity Bundle (9142-01) and Empiria studio software installed + 10 additional user licenses (9141-507E)

ATTACHMENT 1

Salient Characteristics:

This requirement is for the following brand-name or equal products:

Product Brand-Name: Odyssey DLx Manufacturer: LI-COR, Inc.

Part No. 9142-01 Quantity: One (1)

Product Brand-Name: Empiria Studio Software, electronic user licenses Manufacturer: LI-COR, Inc.

Part No. 9141-507E Quantity: Ten (10) licenses

The salient physical, functional, or performance characteristics that brand equal products must meet are as follows:

• Laser-based near infrared imager with multiplexing option

• Functionality to detect/image chemiluminescent and fluorescent western blots, protein arrays, ELISAs, gels, tissue sections, ex-vivo whole organs, multi-well plates for IN-cell, ON-cell and RNAi analysis

• Image field size – 25cm x 25 cm

• A sealed, flat image scanning surface to accommodate different sample types – gels, blots, plates, slides

• Dynamic Detection range – 4 logs manual and >6 logs auto

• Scanning Speeds of 5 – 40cm/s

• Resolution minimum of 21 μm

• Desktop operating system Windows 10 or higher

• Integrated data analysis software

Quantity:

One imaging system bundle with Empiria software installed, and 10 additional software user licenses

Delivery Date:

Within thirty (30) days after receipt of order (ARO)

Period of Performance:

N/A

Option(s):

N/A

ATTACHMENT 1