D-Attachment-PURCHASE DESCRIPTION Keyence All-in-One Fluorescence Microscope.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The Laboratory of Molecular Biology & Immunology

(LMBI), and other labs in National Institute on Aging (NIA) require a stand-alone microscope with its own enclosed dark room with modular slide holders compatible with tissue culture flasks and plates, multiwell plates, coverslips and slides.

2. Background Information and Objective: The Keyence All-in-one microscope will allow complete imaging of tissue microarrays mounted on slides, multi-well experiments assessing the role of translational senescence in a multitude of cells including primary fluorescent cells from mice as well as cell lines such as hVSMCs, Wl38, IMR90, human muscle fibroblast and others. This imaging needs to be analyzed with both the hybrid and macro cell counting software, with the option of lenses ranging from 4X, 10X, 20X and 40X. Filters needed for the fluorescence to be measured include DAPI, GFP, Texas Red, and Cy5. The Laboratory of Molecular Biology & Immunology (LMBI) has plans to use this microscope for screening purposes in 96 and 384 well plates.

3. Generic Name of Product: All-in-one fluorescence microscope

4. Purchase Description: Keyence All-in-One Fluorescence Microscope

5. Salient characteristics: Encapsulated microscope that can be used in ambient lighting, and is capable of z-stack image capturing of fluorescently labeled samples. Software required for analysis of images.

Must include a specimen enclosure to provide an observation environment that, even without a darkroom, will not impair the background contrast required for fluorescence observation.

Must include, as standard components, an electronic shutter, XY and Z motorized stages, an electronic revolver, an electronic filter turret, electronic color filters, electronic phase-contrast slit switching mechanism, electronic dimmer filters, and an electronic aperture control; it is necessary that all of these components can be operated with the mouse.

The microscope must be able to recall all capture settings which include the shutter, aperture, light intensity, XYZ stage position, magnification, filter, imaging mode (brightfield/fluorescence/phase).

The stroke of the motorized XY stage must be at least 114 mm x 80 mm and the minimum travel pitch must be 1 µm or smaller. Also, the stroke of the motorized Z axis stage must be at least 8 mm and the minimum travel pitch must be 0.1 µm or smaller.

The installation space of the microscope unit must be within 500 mm (H) x 350 mm (W) x 500 mm (D) when the panel is closed.

Fluorescence filter replacement must be possible at four locations.

Up to six objective lenses whose magnifications are from 2x to 1 00x must be mountable.

The microscope must be equipped a "low photobleach mode" which physically blocks the light to the sample when it is just stationary. Light is only let through when we move the sample or change the capture settings.

The microscope must be equipped with a monochrome CCD that is cooled to a temperature that is lower than the ambient temperature by at least 25°C. The microscope must also be furnished with a mechanism that can capture full-color images using color filters inserted under electronic control.

The microscope must be equipped with a navigation function that enables the user the following: to make observations at a high magnification while checking the position over the entire region using a wide-area map image linked with information on the XY stage coordinates and to move the observation position by clicking the desired point on the wide-area map image. It must also be possible to create the wide-area map image using any of the mountable lenses.

The microscope must include a function that enables the user, when using any of the mountable lenses, to set a maximum range of 50,000 x 50,000 pixels automatically by clicking the edges of the desired capture range, capture images in this range, and stitch them seamlessly.

The number of pixels that can be recorded in a single image must be at least 12,000,000.

The analysis software must include some basic image processing software such as Haze reduction (no-neighbor deconvolution), image overlays, scale bar, cut/paste, zoom, lookup table, black balance.

6. Quantity:

BZX Fluorescence Microscope BZX / Microscope Head Part# BZ-X810 (QTY=1)

BZX Fluorescence Microscope BZX / Microscope Controller Part# BZ-X800LE (QTY=1)

BZ Support Package Part# BZ Support Package (QTY=1)

BZX Hardware Module / BZX Multi-Stack Module Part# BZ-H4XD (QTY=1)

BZX Hardware Module / BZX Image Cytometer Module Part# BZ-H4XI

BZX Software / BZX Hybrid Cell Count Software Part# BZ-H4C (QTY=1)

BZX Software / BZX Analyzer Software Part# BZ-H4A

BZX Accessory / BZX DAPI Filter Part# OP-87762 (QTY=1)

BZX Accessory / BZX GFP Filter Part# OP-87763 (QTY=1)

BZX Accessory / BZX Cy5 Filter Part# OP-87766 (QTY=1)

BZX Accessory / BZX Texas Red Filter Part# OP-87765 (QTY=1)

BZ Objective Lens (10x Phase) / Plan Fluorite 10x PH Part# BZ-PF10P (QTY=1)

BZ Objective Lens (20x Phase) / Plan Fluorite 20x LD PH Part# BZ-PF20LP (QTY=1)

BZ Objective Lens (40x Phase) / Plan Fluorite 40x LD PH Part# BZ-PF40LP (QTY=1)

BZ Objective Lens (60x Oil) / Plan Apochromat 60x Oil Part# BZ-PA60 (QTY=1)

BZ Objective Lens (4x Phase) / Plan Fluorite 4x PH Part# BZ-PF04P (QTY=1)

Win10 PC for BZ-X800 Part# 972326

7. Delivery Date: 30 days after receipt of order

8. Period of Performance: N/A

9. Option(s): N/A