D-Attachment-Purchase Description Chromium Cell Kits.pdf
PDF 515 KB Posted
- Attached to
- Chromium Cell Kits Federal contract opportunity
- Solicitation number
- 75N95022Q00213
About this file
This document is a combined synopsis/solicitation for commercial items from the Department of Health and Human Services National Institutes of Health National Institute on Drug Abuse. It requests quotes for Chromium Cell Kits to be used for single nuclei RNA and ATAC sequencing to identify gene expression and chromatin accessibility patterns across the human frontal cortex. Required are various 10X Genomics brand name products including Chromium Next GEM Single Cell Multiome ATAC and Gene Expression Reagent Bundles, Chromium Next GEM Chips, and associated index kits. Quotes are due by 2:00 pm Eastern Time on May 20th, 2022 and should be submitted electronically to Rashiid.Cummins@nih.gov. Delivery of the first order is required within 30 days of award and the second order within 90 days. The award will be a firm-fixed-price purchase order evaluated based on technical capability, past performance, and price with technical and past performance significantly more important than price.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| D-Attachment-FAR_52.204-24 (Nov 2021).pdf | ||
| D-Attachment-Invoicing Instructions Without IPP 4-21-22.pdf | ||
| D-Attachment-FAR_52.204-26 (Oct 2020).pdf | ||
| D-Attachment HHS_Subk_plan.docx | DOCX document | |
| D-Attachment-FAR 52.212-5 (Jan 2022).pdf | ||
| D-Attachment-Invoicing Instructions With IPP 4-21-22.pdf | ||
| D-Attachment-FAR 52.212-4 Addendum.pdf |
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Text version
Version 11-30-2016-1 FAR Part 11—Describing Agency Needs
PURCHASE DESCRIPTION
1. Statement of Need and Purpose: This acquisition is necessary to provide supplies and reagents to the Center for Alzheimer’s Disease and Related Dementias (CARD) for neurodegenerative disease research for gene expression analysis.
2. Background Information and Objective: imaging technology improves to capture CARD aims to perform single nuclei RNA and ATAC sequencing for these samples to identify gene expression and chromatin accessibility patterns across the human frontal cortex. CARD anticipates that this resource will substantially accelerate the pace of mechanistic discovery regarding how neurodegenerative diseases mutations cause disease.
3. Generic Name of Product: Chromium cell kits
4. Purchase Description:
Cat no Quantity Chromium Next GEM Single Cell Multiome, ATAC + Gene Expression Reagent Bundle, 16rxn 1000283 12
Chromium Next GEM Chip J Single Cell Kit, 48 rxn 1000234 4
Dual Index Kit TT Set A 96 rxns 1000215 3
Single Index Kit N Set A, 96 rxn 1000212 3 Chromium Next GEM Single Cell Multiome, ATAC + Gene Expression Reagent Bundle, 16rxn 1000283 13
Chromium Next GEM Chip J Single Cell Kit, 48 rxn 1000234 4
Dual Index Kit TT Set A 96 rxns 1000215 2
Single Index Kit N Set A, 96 rxn 1000212 2
Shipping n/a 1
Salient characteristics All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.
Vendor must be able to provide these specifications:
Version 11-30-2016-1 FAR Part 11—Describing Agency Needs
Shall deliver paired full-length T-cell receptor alpha and beta chain sequences at the single cell level using universal and unbiased amplification strategies, thus avoiding amplification and detection bias associated with complex multiplex PCR while revealing deeper biological complexity of antigen specificity on a per-cell basis than single-chain (e.g. TCRβ) methods.
Shall incorporate a unique molecular identifier (UMI) in the template switch oligo enables transcript quantification and further reduces systematic bias by controlling for non-biological SNVs or other variants introduced by PCR and sequencing error.
Shall enable the detection of germline and somatic variation across full-length antigen receptor V(D)J gene sequences.
Shall identify both productive and non-productive recombination events as well as adaptive immune cells with more than one productive T-cell receptor alpha or beta chain.
Shall map transcription start sites
5. Delivery Date: Vendor shall ship according to schedule below.
Ship date
Chromium Next GEM Single Cell Multiome, ATAC + Gene Expression Reagent Bundle, 16rxn 1000283 12
30 days after completed PO
Chromium Next GEM Chip J Single Cell Kit, 48 rxn 1000234 4
30 days after completed PO
Dual Index Kit TT Set A 96 rxns 1000215 3 30 days after completed PO
Single Index Kit N Set A, 96 rxn 1000212 3 30 days after completed PO
Ship date
Chromium Next GEM Single Cell Multiome, ATAC + Gene Expression Reagent Bundle, 16rxn 1000283 13
90 days after completed PO
Chromium Next GEM Chip J Single Cell Kit, 48 rxn 1000234 4
90 days after completed PO
Dual Index Kit TT Set A 96 rxns 1000215 2 90 days after completed PO
Single Index Kit N Set A, 96 rxn 1000212 2 90 days after completed PO
6. Period of Performance: N/A
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