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This document describes a purchase description for a multi-chamber pump controlled cellular coagulation/thrombosis assay device with microscope adapter and analytical software. The National Center for Advancing Translational Sciences is seeking to procure a Venaflux Starter 1.0 kit from Cellix with a Mirus Evo pump and multiflow8 software to enable semi-automated flow controlled thrombosis and coagulation assays using cultured human cells visualized through an Echo Revolve microscope. The kit must facilitate thrombosis, platelet adhesion and aggregation assays from 2.25 to 450 dyne/cm and rolling, adhesion, and migration assays from 0.05 to 10 dyne/cm using biochips enabling multiplexed testing of multiple cell types or adhesion molecules simultaneously. Additional requirements include temperature control, automated cell analysis capabilities, and a one year warranty. Delivery is required within 30 days of order placement for one kit and two packages of consumables.

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Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: NCATS is in need of an apparatus for measuring Thrombosis/Clotting in an in vitro environment with model human cells as part of the work for the cardiovascular working group in the Tox21 consortium.

2. Background Information and Objective: The cardiovascular working group of Tox21 is exploring the effects of exposure brought about by environmental, pharmaceutical, and other means in controlled human cellular models. NCATS has performed a search for an apparatus that can facilitate the measurement of thrombosis/clotting in a human vascular cell model in a quantitative and reproduceable manner suitable for higher throughput.

3. Generic Name of Product: Multichamber Pump controlled cellular Coagulation/Thrombosis assay device with microscope adapter and analytical software.

4. Purchase Description: Cellix Venaflux Starter 1.0 with Mirus Evo Pump with multiflow8 (Brand name or equal) for software-controlled flow. Includes Biochip Frame P Vena8 for Revolve Microscope, Glass Syringe for pump, and tubing kit, power supply and cables. Training and 2 sets of consumables (20 assays) will be included. One year warranty included.

5. Salient characteristics The Venaflux Starter kit will include all parts necessary for semiautomated flow-controlled thrombosis/coagulation assays with cultured cells that can be viewed and assayed through a computer connected to an Echo Revolve microscope (microscope is already part of NCATS inventory).

• Thrombosis, Platelet Adhesions and Aggregation Assays: 2.25 - 450 dyne/cm with Vena8 Fluoro+ Biochips

• Cell-ligand & Cell-Cell Rolling, Adhesion & Migration Sear Flow Assays: 0.05 - 10 dyne/cm

• Multiplexed Assays providing equal flow rates in 8 channels of biochips. This enables multiple cell types or adhesion molecules to be tested simultaneously comparing different therapeutic treatments.

• Temperature controlled conditions mimicking physiological conditions with microenvironmental chamber.

• Cell analysis of % thrombus coverage and number of thrombi: number of cells adhered to protein-coated channels and analysis of rolling velocities of cells

• Programmable with PC controlled software enabling executing of customize protocols.

• Included one year warranty.

• Delivery

6. Quantity: One kit with 2 consumable items packages

• Quantity 1- Cellix Venaflux Starter 1.0 with Mirus Evo Pump with multiflow8 (Brand Name or Equal)

• Quantity 2-Consumable items packages

• Training for the Assay Device

• Delivery

7. Delivery Date: 30 days after order is placed

8. Option(s): <NA

File details come from the government source that posted it. Updated .